Effect of host breeds on gut microbiome and serum metabolome in meat rabbits

Ye, Xiaoxing; Zhou, Liwen; Zhang, Yao; Xue, Shuaishuai; Gan, Qian Fu; Fang, Shouguo

doi:10.1186/s12917-020-02732-6

Cited by 22 publications

(26 citation statements)

References 77 publications

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“…In our study, some species that are known as SCFAs (e.g. butanoate) producer [Roseburia hominis (32,33), Odoribacter splanchnicus (34), Ruminococcus callidus (35), Lachnospiraceae bacterium 3_1_46FAA (36), Bacteroides uniformis (37) and Butyricimonas synergistica (38)], together with the metabolic pathway of producing butanoate (CENTFERM-PWY and PWY-6590) were enriched in the healthy controls. Importantly, both of the two pathways had significantly positive correlations with the above bacterials.…”

Section: Discussionmentioning

confidence: 67%

Structural and Functional Alterations of Gut Microbiota in Males With Hyperuricemia and High Levels of Liver Enzymes

et al. 2021

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Objective: To investigate the correlation between the structure and function alterations of gut microbiota and biochemical indicators in males with hyperuricemia (HUA) and high levels of liver enzymes, in order to provide new evidences and therapeutic targets for the clinical diagnosis and treatment of HUA.Methods: A total of 69 patients with HUA (HUA group) and 118 healthy controls were enrolled in this study. Their age, height, waist circumference, weight, and pressure were measured. The clinical parameters such as fasting plasma glucose (FBG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum uric acid (SUA), serum creatinine (Scr), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), white blood cell (WBC), platelet (PLT), and absolute value of neutrophils (NEUT) were examined. We used whole-genome shotgun sequencing technology and HUMAnN2 MetaCyc pathway database to detect the composition and pathways of the gut microbiota. The main statistical methods were student's t test, chi-square tests, and Wilcoxon rank sum test. The correlations among bacterial diversity, microbial pathways, and biochemical indicators were evaluated by the R function “cor.test” with spearman method.Results: The gut bacterial diversity in HUA group reduced significantly and the community of the microbiota was of significant difference between the two groups. The pathways that can produce 5-aminoimidazole ribonucleotide (PWY-6122, PWY-6277, and PWY-6121), aromatic amino acids, and chorismate (COMPLETE-ARO-PWY, ARO-PWY, and PWY-6163) were enriched in the HUA group; while the pathways that can produce short-chain fatty acids (SCFAs, such as CENTFERM-PWY and PWY-6590) and the gut microbiotas that can produce SCFAs (Roseburia hominis, Odoribacter splanchnicus, Ruminococcus callidus, Lachnospiraceae bacterium 3_1_46FAA, Bacteroides uniformis, Butyricimonas synergistica) and equol (Adlercreutzia equolifaciens) were enriched in healthy controls.Conclusion: The structure and function of the gut microbiota in males with HUA and high levels of liver enzymes have altered apparently. In-depth study of related mechanisms may provide new ideas for the treatment of HUA.

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Section: Discussionmentioning

confidence: 67%

Structural and Functional Alterations of Gut Microbiota in Males With Hyperuricemia and High Levels of Liver Enzymes

et al. 2021

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“…Alloprevotella can produce moderate amounts of acetate and succinate and decrease lifetime cardiovascular disease risk ( Downes et al, 2013 ; Ricaboni et al, 2016 ; Xin et al, 2019 ). Some genera such as Coprococcus and Ruminiclostridium have been demonstrated to produce short-chain fatty acids, which is beneficial to regulate energy intake and maintain the morphology and function of intestine and intestinal epithelial cells ( Tan et al, 2014 ; Ye et al, 2021 ). Moreover, Ruminiclostridium , as an intestinal beneficial bacterium, is involved in the positive regulation of the growth performance and decrease of gastrointestinal diseases of animals ( Tan et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated Bacterial and Fungal Diversity Analysis Reveals the Gut Microbial Alterations in Diarrheic Giraffes

Liu

et al. 2021

Front. Microbiol.

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Gut microbiota has been demonstrated to be associated with multiple gastrointestinal diseases, but information regarding the gut microbial alternations in diarrheic giraffe remains scarce. Here, 16S rDNA and ITS gene amplicon sequencing were conducted to investigate the gut microbial composition and variability in diarrheic giraffes. Results demonstrated that Firmicutes and Proteobacteria were the most dominant phyla in the gut bacterial community, whereas Ascomycota and Basidiomycota were observed to be predominant in the gut fungal community regardless of health status. However, the species and relative abundance of preponderant bacterial and fungal genera in healthy and diarrheic giraffes were different. In contrast to the relatively stabilized gut fungal community, gut bacterial community displayed a significant decrease in the alpha diversity, accompanied by distinct changes in taxonomic compositions. Bacterial taxonomic analysis revealed that the relative abundances of eight phyla and 12 genera obviously increased, whereas the relative abundances of two phyla and eight genera dramatically decreased during diarrhea. Moreover, the relative richness of five fungal genera significantly increased, whereas the relative richness of seven fungal genera significantly declined in diarrheic giraffes. Taken together, this study demonstrated that diarrhea could cause significant alternations in the gut microbial composition of giraffes, and the changes in the gut bacterial community were more significant than those in the gut fungal community. Additionally, investigating the gut microbial characteristics of giraffes in different health states is beneficial to provide a theoretical basis for establishing a prevention and treatment system for diarrhea from the gut microbial perspective.

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“…In this study, we also found that the relative contribution to GH5_1 of R. albus reaches 72.11% and only R. callidus contains GH48 family in all potential bacterial biomarkers of H_NDF group. R. callidus is a producer of short-chain fatty acids (SCFAs), which can be used as a bacterial biomarker for improving the health and production performance of meat rabbits ( Ye et al, 2021 ). S. bovis is a kind of separable bacteria existing in the rumen contents of yak, which can utilize cellobiose, arabinose, mannose, glucose, etc.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs

Liu

Hou

et al. 2021

Front. Microbiol.

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Making full use of high fiber and low-cost crop coproducts is helpful to alleviate the situation of people and livestock competing for crops. Digestion of dietary fibers in pigs is mainly through microbial fermentation in the large intestine. To reveal microbiota related to fiber digestion in pigs, fecal samples have been collected from 274 healthy female Suhuai pigs at 160 days of age under the same feeding conditions and have measured apparent neutral detergent fiber (NDF) and acid detergent fiber (ADF) digestibility. Samples from Suhuai pigs with extreme high and low apparent NDF digestibility and extreme high and low apparent ADF digestibility were subjected to shotgun metagenomic sequencing. At the species level, 62 microbial species in H_NDF group and 54 microbial species in H_ADF group were related to high fiber digestibility. Among them, Lachnospiraceae bacterium 3-1 and Alistipes sp. CAG:514 may be new types of microorganisms associated with fiber digestion. In addition, we found that more abundant GH5 and GH48 family (contribute to cellulose degradation) genes, GH39 and GH53 family (contribute to hemicellulose degradation) genes in microorganisms may contribute to the higher apparent NDF digestibility of pigs, and more abundant GH3 and GH9 family (contribute to cellulose degradation) genes in microorganisms may contribute to the higher apparent ADF digestibility of pigs. The abundance of AA4 family (helps in lignin degradation) genes in H_NDF and H_ADF groups was significantly higher than that in L_NDF and L_ADF groups, respectively (P < 0.05). Three pathways in H_NDF group and four pathways in H_ADF group are important pathways associated with degradation of non-starch polysaccharides, and their relative abundance is significantly higher than that in L_NDF and L_ADF groups, respectively. Gut microbiota of Suhuai pigs with high apparent fiber digestibility had higher abundance of genes and microbiota related to fiber digestion and may have stronger fiber digestion potential compared with low apparent fiber digestibility group. This study revealed that the characteristics of gut microbiota and microbial gene functions of pigs with high fiber apparent digestibility, which provided a theoretical basis and reference for further understanding the impact of gut microbiota on fiber digestibility of pigs.

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Effect of host breeds on gut microbiome and serum metabolome in meat rabbits

Cited by 22 publications

References 77 publications

Structural and Functional Alterations of Gut Microbiota in Males With Hyperuricemia and High Levels of Liver Enzymes

Structural and Functional Alterations of Gut Microbiota in Males With Hyperuricemia and High Levels of Liver Enzymes

Integrated Bacterial and Fungal Diversity Analysis Reveals the Gut Microbial Alterations in Diarrheic Giraffes

Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs

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