Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes

Reyes, M. De los; Lange, Johanna de; Miranda, Pedro; Palominos, Jaime; Barros, C.

doi:10.1016/j.theriogenology.2004.11.008

Cited by 43 publications

(55 citation statements)

References 38 publications

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“…Studies reveled that in vitro nuclear maturation was not influenced by the reproductive status of donor females and that the hormonal environment of in vitro culture was not a reliable marker of oocytes maturation competence. It may be suggested that the quality of oocytes recovered from the bitches is a more important indicator of following stages of proper IVM than the addition of gonadotropine hormones into the culture media (De Los Reyes et al 2005). Similar results were obtained by Luvoni et al (2001), who compared different in vitro meiotic competence and reaching MII stage by canine oocytes isolated from donors at various oestrus stages.…”

Section: The Role Of Selected Media Supplements In Canine Oocytes Matsupporting

confidence: 69%

The in vitro culture supplements and selected aspects of canine oocytes maturation

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Polish Journal of Veterinary Sciences

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The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved.In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.

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Section: The Role Of Selected Media Supplements In Canine Oocytes Matsupporting

confidence: 69%

The in vitro culture supplements and selected aspects of canine oocytes maturation

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Polish Journal of Veterinary Sciences

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“…These COCs were placed in 35-mm Petri dishes (Falcon # 3001; Becton-Dickinson, Lincoln Park, NY, USA) containing PB1 medium [47] supplemented with 3 mg/mL BSA (Sigma, St Louis, MO, USA) and 100 lg/mL streptomycin (Meiji, Tokyo, Japan), and examined under a dissecting microscope (SMZ1500; Nikon Instech, Tokyo, Japan). After three washes in the same medium, the COCs were selected according to the criteria previously described by De los Reyes et al [18], Hewitt and England [48], and Otoi et al [49] under an inverted microscope (DMIR/E, Leica, Wetzlar, Germany). The conditions were those reported to favor meiotic competence based on the uniformity of ooplasm and cumulus cell complement, homogeneous dark cytoplasm with three or more layers of compact cumulus cells, and oocytes [110 lm in diameter.…”

Section: Methodsmentioning

confidence: 99%

“…The oocytes were subsequently overlaid with a coverslip. The chromatin state was evaluated using a fluorescence microscope with UV light (C-SHG1, Nikon) to determine the meiotic stage as described by Hewitt and England [48] and De los Reyes et al [18]: 1 immature or germinal vesicle (GV, Fig. 1a), when the nuclear envelope was visible; 2 resumption of meiosis or germinal vesicle breakdown (GVBD, Fig.…”

Section: Assessment Of Nuclear Statusmentioning

confidence: 99%

“…Since that report, and despite numerous studies documenting and focusing on establishing a suitable system for IVM of canine oocytes [15][16][17][18][19], the efficiency is lower than for other domestic mammalian species, for example cattle [20,21], sheep [22], pigs [23], mice [24], and cats [25]. Although puppies have been produced from somatic cell nuclear transfer of in-vivo matured oocytes [26], there has been no report of the production of live young after transfer of in-vitro maturation and fertilization-derived embryos [27].…”

Section: Introductionmentioning

confidence: 99%

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Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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Purpose To investigate the ability of medium conditioned with bovine cumulus-oocyte complexes (COCs) to support nuclear maturation of canine oocytes recovered from domestic dog ovaries. Methods Cumulus-oocyte complexes were obtained from ovaries of domestic bitches (8 months old to 7 years old), and in-vitro maturation was evaluated in TCM-199 supplemented with different concentrations (0, 20, 30 or 50%) of bovine COCs-conditioned medium (BCM). The canine COCs were cultured for 72 or 96 h at 38.5°C in 5% CO 2 , 5% O 2 and 90% N 2 . The bovine COCs-conditioned medium was obtained from culture of bovine COCs with TCM-199 supplemented with 5% FCS for 22 h at 38.5°C in 2% CO 2 , 98% air. Results The proportion of germinal vesicle breakdown (GVBD) after 72 h was significantly higher (P \ 0.05) in medium supplemented with 30% BCM (20.7%) compared with the control group (13.4%). The rates of GVBD-MII stage were significantly higher (P \ 0.05) when oocytes were matured with BCM at concentration of 30% (41.5%) compared with control (26.6%) after 72 h in-vitro culture. After 96 h in-vitro culture, the oocytes matured in medium supplemented with 30% BCM (5.5%) showed a significant increase (P \ 0.05) in the proportion of MII compared with control (0.7%). However, increasing the cultivation time from 72 to 96 h resulted in an increase in oocyte degeneration rate. Conclusions The results suggested that bovine COCsconditioned medium supplementation significantly increased nuclear maturation of canine oocytes.

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“…For example, the age of the donor bitch [3], oocyte diameter [4,5], protein [6][7][8][9][10] and hormonal supplementation of maturation media [11][12][13][14][15][16] have all been examined. However, the proportion of MII oocytes remains low (0-43.4%) [9,[15][16][17][18][19][20], and a number of essential issues have yet to be resolved.One of these issues is the optimal culture period for IVM of canine oocytes. When oocytes are recovered directly from ovarian follicles and cultured in vitro, some progress to the MII stage after as little as 24 h of culture [17,21,22], and de los Reyes et al [15] demonstrated that some of oocytes reach the MII stage after 96 h of culture.…”

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confidence: 99%

Kinetics of Nuclear Status and Kinase Activities During In Vitro Maturation of Canine Oocytes

Suzukamo

Hoshina

Moriya

et al. 2009

Journal of Reproduction and Development

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Abstract. In contrast to those of other mammals, canine oocytes are ovulated at the germinal vesicle (GV) stage and then progress to the metaphase II (MII) stage in the oviduct. In other species, oocytes at the MII are widely used for in vitro fertilization or as recipients in somatic cell nuclear transfer. Many researchers have tried to improve the in vitro maturation (IVM) of canine oocytes. However, the proportion of MII oocytes remains low, resulting in poor efficiency of embryogenesis in vitro. This leads us to the possibility that the in vitro cytoplasmic maturation of canine oocytes is insufficient. Furthermore, the optimal culture period for IVM of canine oocytes is controversial, and physiological evaluation is required to improve canine IVM. We show here the time-dependent changes in mitogen-activated protein kinase (MAPK) and p34 cdc2 kinase activities in canine oocytes during IVM, since it is well known that both MAPK and p34 cdc2 kinase are activated following meiotic progression and show high activities in the MII stage in other species. Immediately after collection from ovaries, most oocytes were arrested at the GV stage, which was maintained until 24 h of culture. At 48 h of culture, more than half of the oocytes had progressed beyond the MI stage. A higher proportion of MII oocytes were observed with 72 h of culture compared with other culture periods. MAPK activity was found to increase in a time-dependent manner and reached a plateau at 72 h of culture. The level of p34 cdc2 kinase activity also increased in a time-dependent manner, with its maximal level observed after 72 h of culture. Activity was decreased with 96 h of culture, although there was no significant difference in the proportion of MII oocytes between 72 and 96 h. Our data thus show that the optimal culture period for IVM of canine oocytes is 72 h because both MAPK and p34 cdc2 kinase showed high activities at that time. Key words: Canine, In vitro maturation, Kinase, Oocyte (J. Reprod. Dev. 55: [116][117][118][119][120] 2009) ocytes in most mammalian species progress to the metaphase II (MII) stage in follicles and are then ovulated into the oviduct; however, canine oocytes are ovulated as immature oocytes at the germinal vesicle (GV) stage. These ovulated canine oocytes require at least 48 h to progress to the MII stage within the oviduct [1,2]. Many researchers have reported the in vitro maturation (IVM) of canine oocytes, and the discussion in the literature now focuses on how to improve development. For example, the age of the donor bitch [3], oocyte diameter [4,5], protein [6][7][8][9][10] and hormonal supplementation of maturation media [11][12][13][14][15][16] have all been examined. However, the proportion of MII oocytes remains low (0-43.4%) [9,[15][16][17][18][19][20], and a number of essential issues have yet to be resolved.One of these issues is the optimal culture period for IVM of canine oocytes. When oocytes are recovered directly from ovarian follicles and cultured in vitro, some progress to the MII stage afte...

show abstract

Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes

Cited by 43 publications

References 38 publications

The in vitro culture supplements and selected aspects of canine oocytes maturation

The in vitro culture supplements and selected aspects of canine oocytes maturation

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Kinetics of Nuclear Status and Kinase Activities During In Vitro Maturation of Canine Oocytes

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