Effect of Hybrid Vigor on Callus Induction and Regeneration of Sugarcane

Sobhakumari, V. P.; Mathew, Sara Deepa

doi:10.1508/cytologia.74.71

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…The MP value derived from the average of two respective parents (P1 and P2). Hence, MPH can be calculated by following Sobhakumari and Mathew [38].…”

Section: Discussionmentioning

confidence: 99%

Diversity and Heterosis of Leaf Anatomical Traits in Backcross 1 (BC1) Derived from Interspecific Hybridization between Commercial Cane (Saccharum spp. Hybrid) and Wild Type (S. spontaneum)

Wiangwiset,

Dermail,

Piwpuan

et al. 2023

Agronomy

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Interspecific hybridization between commercial and wild canes followed by backcrossing may transfer favorable alleles responsible for drought tolerance in sugarcane. Our study aimed to assess the distribution of BC1 individuals on leaf anatomy and to classify them regarding heterosis values. Five BC1 populations were established using a commercial Saccharum spp. hybrid as a donor female and the F1 interspecific hybrids as recurrent males. Leaf anatomy included leaf thickness (LT), cuticle thickness (CT), the vertical length of bulliform cell (VBC), stomatal crypt depth (SCD), percent CT, percent VBC, and percent SCD. The anatomical traits of BC1 showed high phenotypic variations, and all populations can be divided into three groups based on their heterosis values. Heterosis seemed to be genotype and trait dependent as the estimates varied considerably across populations and observed traits, ranging from negative on LT to positive on VBC. Group I (BC1-1) showed positive heterosis on percent CT, percent VBC, and percent SCD. Dendrogram analysis revealed that some clones in population BC1-1 were promising regarding stalk weight and leaf anatomy, making them desirable for further clone selections. Backcrossing with commercial canes resulted in higher BC1 means than their mid-parents despite low heterosis on leaf anatomy.

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“…The MP value derived from the average of two respective parents (P1 and P2). Hence, MPH can be calculated by following Sobhakumari and Mathew [38].…”

Section: Discussionmentioning

confidence: 99%

Diversity and Heterosis of Leaf Anatomical Traits in Backcross 1 (BC1) Derived from Interspecific Hybridization between Commercial Cane (Saccharum spp. Hybrid) and Wild Type (S. spontaneum)

Wiangwiset,

Dermail,

Piwpuan

et al. 2023

Agronomy

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“…Conventional techniques of crop improvement are lengthy processes and have limited success. Plant tissue culture has often been hailed as one of the most significant potential areas for crop improvement and genetic transformation [6]. Callus induction via indirect organogenesis is one of the substantial steps for selecting the suitability of genotypes for tissue culture-based research [7].…”

Section: Introductionmentioning

confidence: 99%

Response of Different Explants for Callus Induction in Cucumber

Sultana

Nahar

Hossain

et al. 2021

EJBIO

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In vitro regeneration of cucumber is relatively difficult for genetic improvement. In this regard, different concentrations of growth regulators and three types of explants (cotyledon, hypocotyl and leaf disc) were investigated for their efficiency on callus induction potential. Among different explants explored for callus induction with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), leaf disc responded earlier (4.67 days) and showed higher percentage of callus induction (91.50%) with 2 mg/l 2,4-D supplemented Murashige and Skoog (MS) media. The same concentration of 2,4-D resulted in the maximum callus fresh (0.56 g) and dry weight (0.39 g) from leaf disc explant. Then the callus was transferred to untreated, 2.0 mg/l BAP + 0.2 mg/l NAA + 1.0 mg/l Kn, 2.0 mg/l BAP + 1.0 mg/l NAA + 1.0 mg/l Kn and 2.0 mg/l BAP + 1.5 mg/l NAA + 1.0 mg/l Kn fortified MS medium. After transferring the callus of different explants to shoot regeneration media containing different concentrations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and Kinetin (Kn), only cotyledon callus started to regenerate shoot. The combination of BAP (2 mg/l) + NAA (0.2 mg/l) + Kn (1 mg/l) showed highest shoot regeneration percentage (67.77%) and the maximum number of shoots (5.12) per explant were recorded in the treatment combination of 2 mg/l BAP + 0.2 mg/l NAA + 1 mg/l Kn. These results provided a basis for the optimization of the callus induction protocol of cucumber for genetic transformation.

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