Effect of hydrophilic groups on the bioactivity of antimicrobial polymers

Abstract

Antimicrobial polymers have recently been investigated as potential treatments to combat multidrug-resistant pathogens. A typical antimicrobial polymer consists of cationic groups that allow the polymers to adsorb onto negatively charged...

Antimicrobial Polymers of Linear and Bottlebrush Architecture: Probing the Membrane Interaction and Physicochemical Properties

“…The most universal principle is the balance between cationic and hydrophobic (and hydrophilic) components of the polymer. [15,17] The cationic unit is essential to initiate the selective binding with negatively charged bacterial cell membranes through electrostatic interactions, and subsequently, the hydrophobic moieties can insert into the lipid bilayer and cause its disruption. [18,19] In addition to the amphiphilic balance, other interconnected properties that may affect the antimicrobial activity, as well as the toxicity profile of the APs, must be carefully considered in the structure design.…”

Effect of Chain Lengths on the Antibiofilm and Hemolytic Activities of Main-Chain Alternating Polysulfoniums

“…However, biological medium, such as bacteria culture broth, is usually rich in protein, which may interact with the cationic and hydrophobic groups in antimicrobial polymers to form polymer–protein complexes (PPCs) via electrostatic and hydrophobic interactions. − The formation of PPCs can impede the cationic groups from interacting with the negatively charged bacterial membranes and lead to a reduced antimicrobial efficiency. Absorbance measurements at 595 nm were thus performed to get an indication of the PPCs formed by alternating polysulfoniums in DI water and tryptic soy broth (TSB) media at different concentrations on the basis of their MBC values. − In DI water, no turbidity was observed for all polysulfoniums, therefore showing that they were well dissolved and did not form large aggregates (data not shown). However, in the TSB media, the PS + 1 and PS + 2 polymers induced the formation of PPCs when the concentration was above 8× MBC, and the PS + 4 polymer formed PPCs at concentrations from 1–16× MBC, as evidenced by the significantly increased absorbance signals (SI, Figure S3).…”

Antimicrobial polymeric composites for high-touch surfaces in healthcare applications

“… Material Microbes Conditions Measures Ref. Functionalized poly-lactic acid films E. coli and Bacillus subtilis Overnight cultures at 0.07 OD 600 , 24 h droplet on polymer contact Plate counts 17 Amphiphilic ternary polymers P. aeruginosa , E. coli , S. aureus Broth dilution method of polymer samples Minimum inhibitory concentration 31 Functionalized polyurethane (PU) S. aureus, E. coli , Vancomycin-resistant Enterococcus, Broth incubation (5 x 10 7 CFU/mL) at 37°C for 16 h Plate count 32 E. coli 10 7 CFU of E. coli in 100 μL deposited on surface after 30 min samples were lightly rinsed Live/dead staining with confocal microscopy 32 SARS-CoV-2, enveloped TGEV, non-enveloped FCV 100 μL of virus suspension were added on the test sample, which were incubated for various times before the virus was recovered by vortexing the sample with medium. 50% tissue culture infective dose (TCID50) assay 32 Functionalized PU films S. aureus , S. epidermis , P. aeruginosa , E. coli 10 6 cfu/mL submerged polymer incubation for 48 h at 37°C.…”