Effect of<i>Moringa oleifera</i>leaves extract on post–thawed semen quality of Senduro Goat

Wahjuningsih, Sri; Ihsan, Muhammad Nur; Ciptadi, Gatot; Isnaini, Nurul; Rahayu, Sri; Afifah, Desy Dwi

doi:10.1088/1755-1315/247/1/012055

Cited by 2 publications

(3 citation statements)

References 8 publications

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“…In addition, all sperm velocity types (VCL, VSL, and VAP) and sperm kinetic indexes were significantly increased by the highest MOLE level (300 µl). Similarly, Wahjuningsih et al (2019) found significant (P<0.05) increase in PM, SL, and MI in semen extended with egg yolk-extender supplemented with MOLE. Addition of 5% MOLE provides the best outcomes when compared to other amounts.…”

Section: Discussionmentioning

confidence: 72%

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Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves

Yousif,

Abdel-Khalek,

elsherbeny

et al. 2023

Journal of Sustainable Agricultural and Environmental Sciences

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The aim of the current study was to assess the impacts of aqueous extract of Moringa oleifera leaves (MOLE) on semen quality and sperm freezing ability of bucks. 5 sexually mature Damascus bucks (65-75 kg live body weight, 2-4 years of age) were used for collecting semen once per week for seven weeks. Only semen with a mass motility of ≥ 70% was pooled. Pooled semen had been divided into 4 aliquots, the free extender (C=control, no MOLE) and 3 extenders supplemented with MOLE at levels of (100, 200, and 300 µl/mL) as an antioxidant. Triscitric acid-extender was used to dilute semen at a rate of 1:20 using different MOLE levels, then chilled to 5 °C for two hours (equilibration period), placed into 0.25 mL straws and finally kept in liquid nitrogen at -196 oC for two weeks. After dilution, equilibration, and thawing, semen was assessed visually and via CASA. The results showed that MOLE, at a level of 300 µl/ml, had a beneficial effect on all sperm characteristics, all types of sperm motility, and normal forms assisted by CASA in post-diluted semen, after equilibration and thawing. The highest MOLE level (300 µl/ml) considerably improved the sperm kinetic indexes and all sperm velocity types (VCL, VSL, and VAP). In conclusion, Moringa oleifera leaves aqueous extract (300 µl) in semen extender of bucks is good tool for improving semen quality and sperm freezing ability of bucks.

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Section: Discussionmentioning

confidence: 72%

“…This might explain why post-thawed semen has reduced PM, SL, and MI. Furthermore, sperm freezing and thawing increases ROS, resulting in impaired sperm function (Wahjuningsih et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves

Yousif,

Abdel-Khalek,

elsherbeny

et al. 2023

Journal of Sustainable Agricultural and Environmental Sciences

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“…The sperm motility was assessed under a light microscope with 400 times magnification (Isnaini et al, 2019). Live sperm and morphologically abnormal sperm percentages were estimated using eosin-nigrosin staining procedure, as described previously (Wahjuningsih et al, 2019).…”

Section: Experimental Designmentioning

confidence: 99%

EFFECTS OF SUPPLEMENTATION OF GUAVA JUICE (Psidium guajava L.) TO THE ANDROMED DILUENT ON THE QUALITY OF INDIGENOUS CHICKEN SEMEN STORED AT ROOM TEMPERATURE

2023

THE JAPS

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Indigenous chicken serve as a highly valuable germplasm for the development of the poultry industry in Indonesia. They also play an important role in rural communities as a source of meat, eggs, and additional income. This study evaluated the semen quality of indigenous chicken in the Andromed diluent supplemented with different levels of guava juice during storage at room temperature (24-25 o C) for 0, 30, 60 or 90 minutes. The semen was collected from an indigenous rooster, aged 2.5 years with body weight of 2.5 kg. A total of 10 ejaculates were collected from this rooster; the frequency of semen collection was twice a week. The semen was diluted in the Andromed diluent supplemented with guava juice at the level of 0 (control), 2, 4, or 6%. Evaluation of semen quality parameters included sperm motility, live sperm, and morphologically abnormal sperm. Data were analyzed by using two-way analysis of variance followed by Duncan Multiple Range Test to compare means. Results showed that regardless of storage time, the use of 6% guava juice had significantly higher (P<0.05) sperm motility and live sperm, but lower abnormal sperm as compared to other treatments. It was also found that regardless of guava juice level, the longer storage time continually decreased (P<0.05) the sperm motility and live sperm, but increased (P<0.05) abnormal sperm. Results also showed that there were interaction effects (P<0.05) between the level of guava juice and storage time on the sperm motility and live sperm. This study provides evidence that the addition of 6% guava juice to the Andromed diluent results in better semen quality for artificial insemination up to 60 minutes of storage at room temperature.

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Effect ofMoringa oleiferaleaves extract on post–thawed semen quality of Senduro Goat

Cited by 2 publications

References 8 publications

Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves

Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves

EFFECTS OF SUPPLEMENTATION OF GUAVA JUICE (Psidium guajava L.) TO THE ANDROMED DILUENT ON THE QUALITY OF INDIGENOUS CHICKEN SEMEN STORED AT ROOM TEMPERATURE

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