Effect of Inoculum Source on the Anaerobic Digestion of Mezcal Vinasses at Different Substrate-Inoculum Ratios

Santos, Mónica López Velarde; Ramos, Eusebio Jr. Ventura; Morales, José Alberto Rodríguez; Hensel, Oliver

doi:10.20937/rica.2020.36.53276

Cited by 6 publications

(7 citation statements)

References 24 publications

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“…Resultados del modelo de crecimiento de Gompertz para la producción de metano de las mezclas de vinazas-estiércol bovino (V-EB) utilizadas en el experimento. The accumulated methane yield was higher with a higher vinasses content because "vinasses are more efficient substrates for anaerobic digestion than other substrates due to the quantity of soluble sugars they contain", contrary to what happened with the cellulose present in the bovine manure (López-Velarde et al, 2020). During the adaptation period, bacteria grow and prepare to hydrolyze organic compounds; it takes longer for complex substrates under acidic pH (Parra-Orobio et al, 2014), such as the vinasses alone.…”

Section: Discussionmentioning

confidence: 99%

“…have been observed in anaerobic digestion bacterial growth curves of substrates with an initial bacterial load, such as bovine manure (Ware and Power, 2017;López-Velarde et al, 2020). This implies that vinasses-bovine manure co-digestion favors the initial presence of a bacterial community for vinasses anaerobic digestion in a shorter time.…”

Section: Methane Yield Kineticsmentioning

confidence: 95%

“…However, the acidic pH and high content of phenolic and ferulic compounds derived from the thermal hydrolysis of lignin, which is present in vinasses, might inhibit methanogenesis (Chávez and Domine, 2010;Paul et al, 2018). Recent research has shown that due to its sugar content, mezcal vinasses inoculated with bovine manure is an efficient substrate for AD (López-Velarde et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Uso de vinazas de mezcal para producir metano por co-digestión con estiércol de bovino

Zelaya-Benavidez¹,

Martínez-Gutiérrez²,

Pérez³

et al. 2022

BIOTECNIA

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The best co-digestion ratio between mezcal vinasses and bovine manure was investigated to produce methane.Five vinasses and bovine manure mixtures in different proportionswere compared (v/v): 25-75, 50-50, 75-25, 100-0, and 0-100, with six replications. As reactors, 496 mL glasscontainers with hermetic seal were used, with 300 mL of active volume under mesophilic conditions (36 ± 1 oC) for 10 days. The pH, total solids, and volatile solids of vinasses,bovine manure, and their mixtures were determined in triplicate.The methane accumulated volume was greater with the 0-100 and 75-25, followed by the 100-0, with 286.23, 286.40, and 225.48 NmL CH4, respectively. Methane yield was higher with 100-0 and 75-25 with 28.27 and 22.77 NmL CH4 g-1volatile solids, respectively. The Gompertz bacterial growthmodel showed that the micro-organisms adaptation periodin vinasses was longer than in bovine manure. Co-digestionof vinasses and bovine manure in proportion 75-25 % improvedthe methane production by 26.7 % with respect to the digestion of vinasses alone, and reduced the adaptation timeof bacteria to vinasses by 4.12 days.

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Section: Discussionmentioning

confidence: 99%

Section: Methane Yield Kineticsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Uso de vinazas de mezcal para producir metano por co-digestión con estiércol de bovino

Zelaya-Benavidez¹,

Martínez-Gutiérrez²,

Pérez³

et al. 2022

BIOTECNIA

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“…5, with pretreatment conditions of pH 6.5, a temperature of 50°C, and a ratio of 1 V SW / 2 V MV [49,50]. Alanis et al [14] obtained the highest yield of 65.91 N mL CH 4 g − 1 VS consumed from residues from a seafood restaurant, and López Velarde et al [51] adapted an inoculum for the degradation of mezcal vinasses in a reactor; they obtained a yield of 217 N mL CH 4 g − 1 VS; however, they obtained a CH 4 concentration 30% lower. It is consolidated that codigestion improved the production yield of CH 4 by volatile solids and the concentration of CH 4 .…”

Section: Yieldsmentioning

confidence: 99%

Improvement in methane concentration in the biogas produced through the co-digestion of waste from the seafood restaurant industry and mezcal vinasses.

Sagrero,

Carmen,

Cortés

2023

Preprint

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The release of waste by the mezcal and seafood restaurant industries, due to their characteristics, generates adverse effects on soils, bodies of water, and air. An important aspect of anaerobic digestion is having an inoculum whose microbial consortium is in suitable conditions for carrying out the anaerobic process in its four stages. Applying the combined pretreatments (chemical, thermal, and volumetric ratio of waste) modifies its bioavailability and, consequently, its consumption rate by the microorganisms in the anaerobic inoculum. This research focused on evaluating the strategy of having an enriched inoculum, co-digestion of waste, and applying pretreatments to methane production in the biogas produced through the BMP test. The development of the tests was carried out considering a Taguchi L4 orthogonal experimental design for the variables in two levels of pretreatment: by temperature, volumetric ratio of waste from the restaurant industry of seafood waste and mezcal vinasses, and chemical by pH adjustment, for an initial concentration of residue mixture of 18 g VS L-1; the monitoring variables were biogas production, methane concentration, volatile solid, pH, conductivity, and alkalinity. The results show that the enriched inoculum used in the test had a pH of 8.18, a conductivity of 19.24 mS cm-1, an alkalinity of 31 g CaCO3 L-1, and an alpha (α) ratio of 0.75; the time in which the BMP test was carried out was of the order of 29 days. At the best test, with pretreatment at a temperature of 50°C, a residue ratio of 1 VSW/ 2 VMV, and a pH of 6.5, a production of 1420.51 ± 0.038 N mL CH4 and an 82.3 % CH4 concentration in the biogas were reached, with a yield of 175.37 N mL CH4 g-1 VS and a degradation constant of 0.14 d-1.

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“…Anaerobic digestion (AD) is a biological process that is used in the treatment and stabilisation of biodegradable wastes in wastewater treatment plants for municipal solid waste, as well as food, agricultural, and industrial wastes [ 1 , 2 ]. The AD process generates biogas and a residue known as digestate, which can be used as a soil conditioner or biofertiliser, depending on the amount of nutrient it contains [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Inoculum-to-Substrate Ratio on Putative Pathogens and Microbial Kinetics during the Batch Anaerobic Digestion of Simulated Food Waste

Otite,

Gandhi,

Agyabeng Fofie

et al. 2024

Microorganisms

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The effects of the inoculum (anaerobic digestion effluent) to substrate (simulated food waste) ratio (ISR) 4.00 to 0.25 on putative pathogens and microbial kinetics during batch mesophilic anaerobic digestion were investigated. Red fluorescent protein labelled (RFPAKN132) Escherichia coli JM105 was introduced as a marker species, and together with the indigenous Clostridium sp., Enterococcus sp., Escherichia coli, and total coliforms were used to monitor pathogen death kinetics. Quantitative polymerase chain reaction was also used to estimate the bacterial, fungal, and methanogenic gene copies. All the ISRs eliminated E. coli and other coliforms (4 log10 CFU/mL), but ISR 0.25 achieved this within the shortest time (≤2 days), while ISR 1.00 initially supported pathogen proliferation. Up to 1.5 log10 CFU/mL of Clostridium was reduced by acidogenic conditions (ISR 0.25 and 0.50), while Enterococcus species were resistant to the digestion conditions. Fungal DNA was reduced (≥5 log10 copies/mL) and was undetectable in ISRs 4.00, 2.00, and 0.50 at the end of the incubation period. This study has demonstrated that ISR influenced the pH of the digesters during batch mesophilic anaerobic digestion, and that acidic and alkaline conditions achieved by the lower (0.50 and 0.25) and higher (4.00 and 2.00) ISRs, respectively, were critical to the sanitisation of waste.

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Effect of Inoculum Source on the Anaerobic Digestion of Mezcal Vinasses at Different Substrate-Inoculum Ratios

Abstract: Efecto de la fuente de inóculo en la digestión anaerobia de vinazas de mezcal a diferentes proporciones sustrato-inóculo

Cited by 6 publications

References 24 publications

Uso de vinazas de mezcal para producir metano por co-digestión con estiércol de bovino

Uso de vinazas de mezcal para producir metano por co-digestión con estiércol de bovino

Improvement in methane concentration in the biogas produced through the co-digestion of waste from the seafood restaurant industry and mezcal vinasses.

Effect of the Inoculum-to-Substrate Ratio on Putative Pathogens and Microbial Kinetics during the Batch Anaerobic Digestion of Simulated Food Waste

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