Effect of Ischemia and Hypertonic Saline Loading on Renal Adenine Nucleotides

Urbaitis, Barbara K.

doi:10.1159/000172921

Cited by 8 publications

(6 citation statements)

References 16 publications

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“…During hypoxia, AMP accumulation and EC is stabilized by a reduction in the TAN pool through a degradation of catalyzed AMP [34], [35]. While our results were in general consensus with these observations, we found no difference in EC between ETK and UW solution preservation at different temperatures.…”

Section: Discussionsupporting

confidence: 86%

Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor

et al. 2012

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Background The aim of this study was to investigate factors that may improve the condition of a marginal kidney preserved with a normothermic solution following cardiac death (CD) in a model of rat kidney transplantation (RTx). Methods Post-euthanasia, Lewis (LEW) donor rats were left for 1 h in a 23°C room. These critical kidney grafts were preserved in University of Wisconsin (UW), lactate Ringer's (LR), or extracellular-trehalose-Kyoto (ETK) solution, followed by intracellular-trehalose-Kyoto (ITK) solution at 4, 23, or 37°C for another 1 h, and finally transplanted into bilaterally nephrectomized LEW recipient rats (n = 4–6). Grafts of rats surviving to day 14 after RTx were evaluated by histopathological examination. The energy activity of these marginal rat kidneys was measured by high-performance liquid chromatography (HPLC; n = 4 per group) and fluorescence intensity assay (n = 6 per group) after preservation with UW or ETK solutions at each temperature. Finally, the transplanted kidney was assessed by an in vivo luciferase imaging system (n = 2). Results Using the 1-h normothermic preservation of post-CD kidneys, five out of six recipients in the ETK group survived until 14 days, in contrast to zero out of six in the UW group ( p <0.01). Preservation with ITK rather than ETK at 23°C tended to have an inferior effect on recipient survival ( p = 0.12). Energy activities of the fresh donor kidneys decreased in a temperature-dependent manner, while those of post-CD kidneys remained at the lower level. ETK was superior to UW in protecting against edema of the post-CD kidneys at the higher temperature. Luminescence intensity of successful grafts recovered within 1 h, while the intensity of grafts of deceased recipients did not change at 1 h post-reperfusion. Conclusions Normothermic storage with extracellular-type solution containing trehalose might prevent reperfusion injury due to temperature-dependent tissue edema.

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Section: Discussionsupporting

confidence: 86%

Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor

et al. 2012

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“…There are more metabolic products related to glomerular filtration rate (Leng et al 1995). One of them is also 5'-AMP (Urbaitis 1984) which is known to increase the adenosine tissue concentrations (Miller et al 1978;Osswald et al 1982) and they both seem to be related to the activity of 5'-nucleotidase (Ramos-Salazar and Baines 1986). In our experiment the presence of 5' -nucleotidase was detected in the cortex tissue of sheep fed the low protein diet only.…”

Section: Discussionmentioning

confidence: 99%

The Distribution of Enzymes in the Kidneys of Sheep Fed a High and Low Protein Diet

Faixová¹,

Levkut²,

Kolodzieyski³

et al. 1998

Acta Vet. Brno

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The distribution of 13 enzymes in the cortex, in the outer medulla and in the inner medulla of kidneys was investigated in sheep fed a high (HP) and low (LP) protein diet. Sheep on HP·diet were given a daily ration with 150.2 gofcrude protein (CP) and 15.2 MJ of digestible energy (DE) while the daily intake of sheep on LP·diet was 58.3 g ofCP and 14.2 MJ of DE. Both groups of animals were fed these diets for at least 6 weeks before the collection of kidneys. Histochemical analysis of enzymes showed that glucose·6·phosphatase and 5' ·nucleotidase were not detectable in the kidney Of sheep fed the HP·diet but they appeared in both the cortex and the outer stripe of the outer medulla of sheep on the LP·diet. Alkaline phosphatase of the inner stripe of the outer medulla and of the base of papilla was found in sheep fed the HP·diet only. Adenosine triphosphatase of the inner medulla appeared in sheep fed the LP·diet only. Changes in the distribution of other studied enzymes were only of a small magnitude. The present results demonstrate the enzyme expression of metabolic adaptation of sheep kidney to a low protein intake associated with an increased renal urea reabsorption. Histochemistry, kidney tissue, renal, metabolic adaptationThe sheep kidney plays an important role in many metabolic and biochemical pathways, mainly in gluconeogenesis, both from amino acid and non-nitrogenous glucose precursors. The adaptive changes in metabolic activity of sheep kidney associated with a modified renal function due to altered dietary protein intake are not fully understood yet. Protein intake in sheep and also in other ruminants has a significant effect on the renal concentrating ability (S chm idt-N ie I sen 1979), on the urea reabsorption in tubules (Faix et al. 1988a) and in renal pelvis (C iri 0 and B oi v i n 1990) as well as on glomerular filtration rate (Leng et al. 1985; Faix et al. 1988b;Skrzypczak et al. 1996). Just an increased delivery of some amino acids to sheep kidney due to their direct intrarenal infusion can induce a considerable change in many renal functions (Leng et al. 1994). The adaptation of sheep kidneys to an increased renal reabsorption of urea during low protein intake is expressed also by morphological changes in the epithelium in some nephron segments in both the cortex and medulla (Kocisova et al. 1997). Therefore it may be assumed that renal urea retention in sheep is accompanied also by changes in expression of enzymes in the kidney tissue.The existence of major biosynthetic and catabolic pathways of intermediary metabolism in the mammalian kidney has been known for a long time. Metabolism of the renal cortex is characterized mainly by fatty acid oxidation and gluconeogenesis (Weideman and Address for correspondence:MVOr. Zita Faixova. esc. Kre b s 1969). In contrast, the renal medulla as the kidney compartment with the largest urea recycling, reveals a high rate of aerobic and anaerobic glucose metabolism and it probably depends on glucose as a respiratory fuel (Lee andPeter 1969; A bodeely and ...

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“…These observations constitute the basis for a hypothesis according to which adenosine plays a role in postischemic vasoconstriction and in tubuloglomerular feedback [22,26]. Ischemia as well as increased tubular work accelerate the catabolism of ATP, thus lead ing to a increased production of adenosine [15,21,29]. Adenosine is supposed to leave the tubular cells and to exert its vascular effects via a yet unidentified receptor [26], 5'-AMP would be the direct precursor of the excess of adenosine produced under the con ditions mentioned.…”

Section: Introductionmentioning

confidence: 99%

“…Adenosine is supposed to leave the tubular cells and to exert its vascular effects via a yet unidentified receptor [26], 5'-AMP would be the direct precursor of the excess of adenosine produced under the con ditions mentioned. In fact the cortical con centration of 5'-AMP increases during renal ischemia [29], 5'-AMP is converted to adeno sine by S'-nucleotidascs. It is.…”

Section: Introductionmentioning

confidence: 99%

Properties of an Ecto-5’-Nucleotidase of the Renal Brush Border

M¹,

Angielski²,

Uc³

1985

Kidney Blood Press Res

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A decrease of glomerular filtration rate can be observed during accelerated catabolism of ATP in kidney. It has been proposed that this effect is due to the increase in the renal production of adenosine from ATP. The last reaction in the pathway concerned is the conversion of 5’-AMP to adenosine. We found that brush border membranes purified from homogenates of the rat renal cortex carry out this reaction. The enzyme involved in the hydrolysis has the characteristic properties of ecto-5’-nucleotidases: It is inhibited by ATP, ADP, and by α, β-methyleneadenosine-5’-diphosphate, and it is not stimulated by magnesium. All catalytic sites are accessible from the outside of the vesicles. The K_m of the enzyme for 5’-AMP is 5.77 µM. The enrichment of the 5’-AMP-hydrolyzing activity in the brush border fraction as compared to the homogenate is 9.2 ± 1.5 times. Histochemical staining of kidney sections reveals hydrolysis of 5’-AMP only at the brush border of the proximal tubule. We conclude that the brush border of the proximal tubule of the rat kidney possesses an ecto-5’-nucleotidase which has the same properties as the ecto-5’-nucleotidases in other tissues.

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Effect of Ischemia and Hypertonic Saline Loading on Renal Adenine Nucleotides

Cited by 8 publications

References 16 publications

Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor

Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor

The Distribution of Enzymes in the Kidneys of Sheep Fed a High and Low Protein Diet

Properties of an Ecto-5’-Nucleotidase of the Renal Brush Border

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