Effect of L-Ascorbyl-2-phosphate-Mg on the Yellowtail Seriola quinqueradiata as a Vitamin C Source.

Abstract

A stable form of vitamin C, L-ascorbyl-2-phosphate-Mg (APM), was tested for efficacy as a vitamin C source in the juvenile yellowtail Seriola quinqueradiata. The fish, weighing 2.67g, were fed 4 fish meal-based diets containing graded levels of supplemental APM (0, 3, 6, and 10mg/100g diet) for 83 days. The fish receiving the diet without supplemental APM showed deficiency symptoms such as reduced growth rate, deformity (lordosis and scoliosis), distorted gill support cartilage (degeneration of the ravine part of gill-cover), high mortality, and so on. The supplementation of 3 to 10mg of APM/100g diet improved the growth and feed efficiency of the fish. The results of the feeding experiment indicated that a supplement of about 3 to 6mg APM/100g diet was sufficient to support good growth and survival and to prevent the gross vitamin C deficiency symptoms of the yellowtail. The supplementation of APM elevated the L-ascorbic acid (AsA) contents of serum and liver, serum alkaline phosphatase activity, and the ratio of hydroxyproline/ proline in the bone. The results of the present study showed that APM can be effectively utilized by the yellowtail as a good replacement for AsA.Since L-ascorbic acid (AsA) is a water-soluble, thermolabile vitamin, and is easily oxidized to an inactive form, it is considerably decreased during diet processing, storage, and exposure to sea water due to exidation and leaching in water

Effects of Dietary Vitamin C and Soybean Lecithin in the Nutrition of Brown Bullhead (Ameiurus NebulosusL.) Fingerlings

“…We found that the highest level of vitamin C content in the liver and muscle tissues were in the diets supplemented with encapsulated and crystallized ascorbic acid, alone or in combination with the soybean lecithin. Similar results were obtained by Kanazawa et al (1992) on Japanese amberjack (Seriola quinqueradiata), El Naggar and Lovell (1991) and by Li et al (1998) in channel catfish (Ictalurus punctatus) and Grant et al (1989) and Matusiewicz et al (1994) on rainbow trout (Oncorhynchus mykiss). Due to the vitamin C deficiency, structural deformities such as scoliosis and lordosis have been observed in channel catfish (Wilson, 1973;Andrews and Murai, 1975;Lim and Lovell, 1978;Wilson et al, 1989).…”

Dietary ascorbic acid requirement for growth of striped catfish,Pangasianodon hypophthalmus(Sauvage, 1878) juveniles

“…Because AA is water soluble, L‐ascorbylpalmitate, L‐ascorbyl sulphate and L‐ascorbyl‐2 polyphosphate generally are used as vitamin C sources in fish feed, but the phosphate derivatives of AA are more effective among all the vitamin C sources (Dabrowski, ; Halver & Hardy, ; NRC, ). Most authors worked in the area of vitamin C nutrition in fish used phosphate derivatives of AA for different fish species, such as Channel catfish, Ictalurus punctatus (Wilson et al., ); Rainbow trout, Oncorhynchus mykiss (Cho & Cowey, ; Matusiewicz, Dabrowski, Volker, & Matusiewicz, ); Tilapia, Oreochromis niloticus (Shiau & Hsu, ; Soliman et al., ); Japanese yellowtail, Seriola quinqueradiata (Kanazawa, Teshima, Koshio, Higashi, & Itoh, ); Mahseer, Tor putitora (Khan et al., ); and Magur, Clarias batrachus (Narra, Rajender, Reddy, Rao, & Begum, ). Therefore, we used L‐ascorbyl‐2 polyphosphate as a AA source in the present study.…”

Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes

“…Phosphate derivatives of AA, which are more stable and bioavailable forms of AA, are currently available and shown to have antiscorbutic activity in channel catfish [5], tilapia [13], rainbow trout [14], yellowtail [15], olive flounder [16] and parrot fish [17].…”