Effect of Laryngeal Squamous Cell Carcinoma Tissue Implantation on the Chick Embryo Chorioallantoic Membrane: Morphometric Measurements and Vascularity

Uloza, Virgilijus; Kuzminienė, Alina; Šalomskaitė-Davalgienė, Sonata; Palubinskienė, Jolita; Balnytė, Ingrida; Ulozienė, Ingrida; Šaferis, Viktoras; Valančiūtė, Angelija

doi:10.1155/2015/629754

Cited by 20 publications

(14 citation statements)

References 43 publications

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“…A CAM model of laryngeal squamous cell carcinoma (LSCC) was reported [22]. Fresh LSCC tissue samples from six patients were implanted onto 15 chick embryo CAMs.…”

Section: Patient Derived Tumor Model Using the Cam Assay: Recent Rmentioning

confidence: 99%

Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Komatsu

Matsumoto

Saitō

et al. 2019

Cells

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Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.

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“…A CAM model of laryngeal squamous cell carcinoma (LSCC) was reported [22]. Fresh LSCC tissue samples from six patients were implanted onto 15 chick embryo CAMs.…”

Section: Patient Derived Tumor Model Using the Cam Assay: Recent Rmentioning

confidence: 99%

Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Komatsu

Matsumoto

Saitō

et al. 2019

Cells

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“…Several previous reports show the feasibility of obtaining tumor growth in the form of nodules on the CAM, starting from different tumor cell lines or tissues 4,6,7 . These studies usually aim to evaluate the morphological and morphometric characteristics of tumor nodules.…”

mentioning

confidence: 99%

Exploitation of the chick embryo chorioallantoic membrane (CAM) as a platform for anti-metastatic drug testing

Pawlikowska

Tayoun

Oulhen

et al. 2020

Sci Rep

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The establishment of clinically relevant models for tumor metastasis and drug testing is a major challenge in cancer research. Here we report a physiologically relevant assay enabling quantitative analysis of metastatic capacity of tumor cells following implantation into the chorioallantoic membrane (CAM). Engraftment of as few as 103 non-small cell lung cancer (NSCLC) and prostate cancer (PCa) cell lines was sufficient for both primary tumor and metastasis formation. Standard 2D-imaging as well as 3D optical tomography imaging were used for the detection of fluorescent metastatic foci in the chick embryo. H2228- and H1975-initiated metastases were confirmed by genomic analysis. We quantified the inhibitory effect of docetaxel on LNCaP, and that of cisplatin on A549- and H1299-initiated metastatic growths. The CAM assay also mimicked the sensitivity of ALK-rearranged H2228 and EGFR-mutated H1975 NSCLC cells to tyrosine kinase inhibitors crizotinib and gefitinib respectively, as well as sensitivity of LNCaP cells to androgen-dependent enzalutamide therapy. The assay was suggested to reconstitute the bone metastatic tropism of PCa cells. We show that the CAM chick embryo model may be a powerful preclinical platform for testing and targeting of the metastatic capacity of cancer cells.

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“…Murphy afirmou que, ao inocular tecido de outros mamíferos no embrião da galinha, haveria ausência de uma resposta imune contra os tecidos inoculados (Ribatti, 2004). Como o sistema linfóide do embrião não está totalmente formado até os últimos dias de incubação, o embrião da galinha pode servir de hospedeiro para tecidos enxertados, sem que haja reações contra o tecido (Uloza et al, 2015).…”

Section: Modelos De Estudo Utilizando Membrana Corioalantóicaunclassified

“…Particularmente nos modelos em que se utilizam fragmentos de tecidos, como é a proposta do presente estudo, a MCA tem sido utilizada principalmente para estudo da viabilidade de tumores (Sys et al, 2012;Sys et al, 2013;Uloza et al, 2015), para a verificação do efeito quimioterápico sobre tumores específicos (Ferician et al,2015;Okamura et al, 1995), para estudo do funcionamento de órgãos, principalmente reprodutores (Uematsu et al, 2014;Isachenko et al, 2013;Qureshi et al, 2008;Vatanparast et al, 2018), e para análise do processo de reparo em vários tecidos (Huxlin et al,1992;Katoh et al,2001), incluindo fragmentos de tecido ósseo (Moreno-Jiménez et al, 2016). O quadro 2.1 descreve alguns desses estudos utilizando fragmentos de tecido inoculados na MCA.…”

Section: Modelos De Estudo Utilizando Membrana Corioalantóicaunclassified

“…A MCA permite o xenotransplante de células e tecidos enxertados com baixos índices de rejeição, sendo de fácil manuseio laboratorial e de baixo custo (Sys et al, 2012;Jedelska et al, 2013). O baixo índice de rejeição ocorre porque o sistema linfóide do embrião ainda está incompleto, e o seu desenvolvimento total se dá em períodos mais tardios da embriogênese, permitindo a observação de xenotransplantes durante vários dias após a inoculação na MCA (Uloza et al, 2015).…”

Section: Introductionunclassified

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Análise histomorfológica e imuno-histoquímica de fragmento de gengiva inserido em membrana corioalantóica de ovos fecundados de galinha

Huang¹

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TUNEL negativas, sugerindo a presença de células epiteliais viáveis e pouco diferenciadas. Concluiu-se que a gengiva foi incorporada à MCA, com redução paulatina da reação inflamatória e revascularização, bem como manteve-se viável nesse ambiente em um período de 5 dias; contudo, sofreu processos de adaptação que incluíram a perda da estratificação epitelial e a redução da celularidade na lâmina própria.Palavras-chave: Gengiva. Ovo. Galinha. Membrana corioalantóica. ABSTRACTHuang I. Histomorphological and imunohistochemistry analysis of gingival fragments inserted in chorioallantoic membrane of chicken eggs [dissertation]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2018. Versão Corrigida. Chorioallantoic membrane (CAM) is one of the chicken embryo extra embryonic membrane, whose main characteristic is being very vascularized and permeable for ionic and gaseous exchanges. The environment where it is inserted allows the development of many experimental model, including studies about angiogenesis, toxicology and biocompatibility, as well as analysis of the repair process and carcinogenesis of many tissues. The objective of this study was to verify the histomorphological changes of gingival fragments grafted on CAM, with the intent to check the viability of this model in studies involving oral mucosa. Fragments of gingiva were collected from patients undergoing dental extraction and grafted on CAM of fertilized chicken egg after 9 days of embryonic development. The collect of gingiva were made after 1, 3 and 5 days of insertion on CAM. The morphological alterations were analyzed over the time in the epithelium and lamina propria of gingiva and CAM. TUNEL analysis were made to evaluate the presence or not of apoptosis in the gingival tissue. Imunohistochemistry and quantification of the expression of keratin 10 (K10) and 14 (K14) were also made to check any alteration of epithelial differentiation, and CD34, for measurement of vascularization. Total of 25 fertilized egg were used, 10 eggs received gingiva for experiments; remaining egg didn't show embrionary viability and were discarded. After 1 day of inoculation, the CAM-gingiva interface exhibited a intense inflammatory infiltrate, which invaded the lamina propria. After 5 days, this inflammatory reaction were restricted to the CAM-gingiva interface. The main morphological changes in the gingiva were observed between 3 to 5 days of inoculation, in which there was loss of epithelial architecture, only the basal layer were viable, and a reduction of cellularity in lamina propria. Were observed a increase of vascularization in period of 5 days, attested by the quantification of positive CD34vessel. The remaining epithelia showed the absence of stratification and expression of K10, intense marking of K14 and TUNEL cell negative, suggesting the presence of viable epithelial cells and less differentiated. It was concluded that the gingiva was incorporated by CAM, with gradual reduction of inflammatory reaction and revascularization, as well remained ...

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Effect of Laryngeal Squamous Cell Carcinoma Tissue Implantation on the Chick Embryo Chorioallantoic Membrane: Morphometric Measurements and Vascularity

Cited by 20 publications

References 43 publications

Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Exploitation of the chick embryo chorioallantoic membrane (CAM) as a platform for anti-metastatic drug testing

Análise histomorfológica e imuno-histoquímica de fragmento de gengiva inserido em membrana corioalantóica de ovos fecundados de galinha

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