Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Komatsu, Aoi; Matsumoto, Kotaro; Saitō, Tsunemasa; Muto, Manabu; Tamanoi, Fuyuhiko

doi:10.3390/cells8050440

Cited by 41 publications

(41 citation statements)

References 29 publications

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“…Chicken CAM assays are commonly used in cancer research [40,41]. Here, to develop the in ovo model for glioblastoma, we implanted GSCs on chicken CAM.…”

Section: Chicken Chorioallantoic Membrane (Cam) Assay For Gscsmentioning

confidence: 99%

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells

Trus

Bérubé

Jiang

et al. 2020

Viruses

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We studied whether cytosine phosphate–guanine (CpG) recoding in a viral genome may provide oncolytic candidates with reduced infection kinetics in nonmalignant brain cells, but with high virulence in glioblastoma stem cells (GSCs). As a model, we used well-characterized CpG-recoded Zika virus vaccine candidates that previously showed genetic stability and safety in animal models. In vitro, one of the CpG-recoded Zika virus variants had reduced infection kinetics in nonmalignant brain cells but high infectivity and oncolytic activity in GSCs as represented by reduced cell proliferation. The recoded virus also efficiently replicated in GSC-derived tumors in ovo with a significant reduction of tumor growth. We also showed that some GSCs may be resistant to Zika virus oncolytic activity, emphasizing the need for personalized oncolytic therapy or a strategy to overcome resistance in GSCs. Collectively, we demonstrated the potential of the CpG recoding approach for oncolytic virus development that encourages further research towards a better understanding of host–tumor–CpG-recoded virus interactions.

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“…Chicken CAM assays are commonly used in cancer research [40,41]. Here, to develop the in ovo model for glioblastoma, we implanted GSCs on chicken CAM.…”

Section: Chicken Chorioallantoic Membrane (Cam) Assay For Gscsmentioning

confidence: 99%

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells

Trus

Bérubé

Jiang

et al. 2020

Viruses

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“…[22] Another is chicken egg tumor model (chorioallantoic membrane (CAM) assay) that provides a simple and versatile system. [23][24][25][26] In this model, the tumor is formed 3-4 days after transplantation of human cancer cells owing to incomplete development of the immune system. [27][28] Meanwhile, the rich angiogenic system that is fully developed in the CAM membrane provides abundant nutrient.…”

Section: Introductionmentioning

confidence: 99%

“…One of them uses tumor spheroids that are generated by culturing cancer cells in a three‐dimensional array . Another is chicken egg tumor model (chorioallantoic membrane (CAM) assay) that provides a simple and versatile system . In this model, the tumor is formed 3–4 days after transplantation of human cancer cells owing to incomplete development of the immune system .…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Periodic Mesoporous Organosilica (BPMO) Loaded with Daunorubicin: A Promising Nanoparticle‐Based Anticancer Drug

et al. 2020

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Biodegradable periodic mesoporous organosilica (BPMO) nanoparticles have emerged as a promising type of nanocarrier for drug delivery, given the biodegradable feature is advantageous for clinical translation. In this paper, we report synthesis and characterization of daunorubicin (DNR) loaded BPMO. DNR was loaded onto rhodamine B‐labeled BPMO that contain tetrasulfide bonds. Tumor spheroids and chicken egg tumor models were used to characterize the activity in biological settings. In the first experiment we examined the uptake of BPMO into tumor spheroids prepared from ovarian cancer cells. BPMO were efficiently taken up into tumor spheroids and inhibited their growth. In the chicken egg tumor model, intravenous injection of DNR‐loaded BPMO led to the elimination of ovarian tumor. Lack of adverse effect on organs such as lung appears to be due to excellent tumor accumulation of BPMO. Thus, DNR‐loaded BPMO represents a promising nanodrug compared with free DNR currently used in cancer therapy. OK

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“…Chicken embryo has been a model organism for developmental biology for a very long time . It is expansively studied in fields of developmental biology , cardiovascular studies , and angiogenesis . Research in chicken embryos has led to many fundamental breakthroughs in developmental and cancer biology including knowledge of formation and septation of heart, discovery of viral oncogenes and identification of transcription factors .…”

Section: Introductionmentioning

confidence: 99%

High resolution, label‐free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell

Sharma

Ishak

Swee‐Hin

et al. 2020

Journal of Biophotonics

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Chicken embryos have been proven to be an attractive vertebrate model for biomedical research. They have helped in making significant contributions for advancements in various fields like developmental biology, cancer research and cardiovascular studies. However, a noninvasive, label-free method of imaging live chicken embryo at high resolution still needs to be developed and optimized. In this work, we have shown the potential of photoacoustic tomography (PAT) for imaging live chicken embryos cultured in bioengineered eggshells. Laser pulses at wavelengths of 532 and 740 nm were used for attaining cross-sectional images of chicken embryos at different developmental stages. Cross-sections along different depths were imaged to gain knowledge of the relative depth of different vessels and organs. Due to high optical absorption of vasculature and embryonic eye, images with good optical contrast could be acquired using this method. We have thus reported a label-free method of performing cross-sectional imaging of chicken embryos at high resolution demonstrating the capacity of PAT as a promising tool for avian embryo imaging. K E Y W O R D S bioengineered eggshell, chicken embryo development, photoacoustic computed tomography, photoacoustic imaging, vasculature imaging

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Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Cited by 41 publications

References 29 publications

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells

Biodegradable Periodic Mesoporous Organosilica (BPMO) Loaded with Daunorubicin: A Promising Nanoparticle‐Based Anticancer Drug

High resolution, label‐free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell

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