Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

Abstract

The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium-aluminum-arsenium (GaAlAs), 780 nm, or indium-gallium-aluminum-phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm(2), 3 J/cm(2) and 5 J/cm(2) (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey's test (P </= 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.

Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

“…23,24 Conversely, increased cell proliferation rates have been observed for LLLT-treated cells at both energy doses selected for use in this study (0.5 and 3 J/cm 2 ), confirming that this type of therapy, at specific parameter levels, may biomodulate cell functions. [13][14][15][16][17][18]25,26 Most of the effects of LLLT have been associated with the activation of mitochondrial cytochrome c oxidase, which leads to increased adenosine triphosphate (ATP) production and the activation of several cell-signalling pathways. 27,28 In the present study, it may be speculated that similar laser biostimulation occurred in those irradiated fibroblasts that presented significantly increased proliferation (Table 3).…”

Viability of fibroblasts cultured under nutritional stress irradiated with red laser, infrared laser, and red light-emitting diode

“…8,9,15 However, in this study only the positive control group had ideal supplementation; all other groups were supplemented with 5% fetal bovine serum, or with nutritional deficit. However, all irradiated groups showed greater fibroblastic survival and possibly cell proliferation than the positive control group.…”

Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)

“…In these experiments, lineage human gingival fibroblasts (FGH), previously obtained from the primary culture (Damante et al 2009) were used. The cells were grown in Eagle"s medium, modified by Dulbecco (DMEM), supplemented with bovine fetal serum 10% and 1% antimycotic antibiotic solution, at 37°C in an incubator with a CO 2 level of 5% atmosphere.…”