Effect of Leukemia Inhibitory Factor on the Myelinogenic Ability of Schwann-Like Cells Induced from Human Adipose-Derived Stem Cells

Abstract

The Schwann cells (SCs) may be obtain from nerve biopsies for autologous transplantation. However, it is difficult to obtain sufficient amount of SCs for clinical applications. Human adipose-derived stem cells (ADSCs) can be induced to differentiate into Schwann-like cells (S-like cells) and used for autologous transplantation. However, effect of leukemia inhibitory factor (LIF) on the myelinogenic ability of SC-like cells induced from human ADSC is not investigated yet. The aim of this study was to evaluate of the effect of exogenous LIF on myelinogenic potential of differentiated cells in vitro. ADSCs were harvested from human fat tissue and characterized using flow cytometry. Human ADSCs were treated for sphere formation and LIF was added to terminal differentiation medium. GFAP/S100β and MBP markers were used to confirm differentiation of human ADSCs, and myelinogenic ability of SC-like cells, respectively, using both immunostaining and real-time RT-PCR analysis. The analysis for GFAP(+)/S100β(+) revealed that LIF can increase both differentiated cells rates and the percentage of myelinating SC-like cells (p < 0.05). Our data showed that SC-like cells induced from human ADSCs were able to generate myelin when exposed to LIF and these cells could be a potential source for the treatment of peripheral and central axonal injuries.

Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells

“…It is determined these isolated cells are stem in the previous study. [24] The cell cultures were kept at a temperature of 37°C in a humid atmosphere with 5% CO 2 . The medium was changed every 3 days.…”

Combination of Acellular Nerve Graft and Schwann Cells-Like Cells for Rat Sciatic Nerve Regeneration

“…ADSCs can be induced and differentiated into Schwann cells-like cells in vitro [22, 23]; in this research, the differentiated cell expresses both S-100 and GFAP that exist in Schwann cells. At the same time, it have been observed that ADSCs can adhere and grow in the scaffold when they were implanted into the acellular nerve scaffold; during this period, the cytoactivity of them was similar to the cells in culture media ( P > 0.05); it means good histocompatibility between cells and acellular nerve scaffold [24, 25].…”

Adipose-Derived Stem Cells for Nerve Repair: Hype or Reality?

“…Zavan et al [2010] showed that proliferating glial-like cells could be isolated from human neurospheres. This method has been adapted and, with the inclusion of leukemia inhibitory factor in the differentiation medium, it was possible to increase the number of S100/GFAP-positive cells and the percentage of myelinating cells [Razavi et al, 2013]. A greater understanding of the mechanisms behind the induction of Schwann cell gene and protein expression is required to develop more effective differentiation protocols for human stem cells.…”