Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine

Amini, Ebrahim; Asadpour, Reza; Roshangar, Leila; Jafari-Joozani, R.

doi:10.29252/ijrm.14.4.255

Cited by 15 publications

(14 citation statements)

References 32 publications

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“…The compound was supplemented into maturation medium; unfortunately, the compound did not exert any positive effect on maturation and development of ovine oocytes. However, a low concentration of the α-linoleic acid did not harm the developmental competence of the oocytes [14]. In contrast, similar experiment was performed with oocytes collected from ovaries of prepubertal lambs [34].…”

Section: Introductionmentioning

confidence: 94%

“…For example, the inappropriate in vitro maturation of oocytes is the main reason of polyspermy after IVF when compared to developmental competence between in vivo and in vitro produced oocytes [10]. To have a glimpse at what progress in the field has been made over the last 5 years, we collected 25 papers on ovine IVM published by different countries through PubMed and listed them in Table 1[11], [12], [13], [14], [15], [16], [17], [18], [19], [20], [21], [22], [23], [24], [25], [26], [27], [28], [29], [30], [31], [32], [33], [34], [35]. It should be noticed that in the table, all blastocyst rates have been standardized, which means that all blastocyst rates presented in Table 1 are calculated based on the number of cleaved embryos.…”

Section: Introductionmentioning

confidence: 99%

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Advances in in vitro production of sheep embryos

Zhu

Moawad

Wang

et al. 2018

International Journal of Veterinary Science and Medicine

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Sheep is an important livestock in the world providing meat, milk and wool for human beings. With increasing human population, the worldwide needs of production of sheep have elevated. To meet the needs, the assistant reproductive technology including ovine in vitro embryo production (ovine IVP) is urgently required to enhance the effective production of sheep in the world. To learn the status of ovine IVP, we collected some publications related to ovine IVP through PubMed and analyzed the progress in ovine IVP made in the last five years (2012–2017). We made comparisons of these data and found that the recent advances in ovine IVP has been made slowly comparable to that of ovine IVP two decades ago. Therefore, we suggested two strategies or approaches to tackle the main problems in ovine IVP and expect that the efficiency of ovine IVP could be improved significantly when the approaches would be implemented.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Advances in in vitro production of sheep embryos

Zhu

Moawad

Wang

et al. 2018

International Journal of Veterinary Science and Medicine

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“…В інших дослідженнях до середовища дозрівання додавали α-лінолеву кислоту -поліненасичену жирну кислоту, яка є у високих концентраціях у фолікулярній рідині. Однак у низькій концентрації α-лінолева кислота не справляла негативного впливу на компетентність розвитку ооцитів (Amini et al, 2016).…”

Section: результати та їх обговоренняunclassified

Modern achievements of the development of scientific research at obtaining in vitro sheep embryos

2020

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New literature data on research aimed at improving the in vitro production of sheep embryos presents in the article. An analysis of the achievements of scientists from different countries to increase the efficiency of the main stages of embryo production in vitro: maturation of oocytes in vitro, their in vitro fertilization and in vitro embryo culture. In the literature experience has shown that the efficiency of oocyte maturation in vitro is significantly influenced by the experience and qualifications of scientists, the age of the egg donor, the improvement of the environment by adding roscovitin to inhibit meiosis, α-linolenic acid, cerium dioxide nanoparticles (CeO2 NPs) and sericin to accelerate nuclear maturation and increase the number of oocytes of the second meiotic metaphase (MII). The main factors influencing the effectiveness of in vitro fertilization have been identified, and the parameters of the limited time of fertilization ability of sperm and the ability of oocytes to fertilize, which is called the “fertile span”, have been determined. The main effective medium that increases the effectiveness of in vitro fertilization – synthetic oviduct fluid (SOF) with the addition of heparin and serum of cattle or sheep. The main parameters of sheep embryo culture in vitro are presented with the definition of the most commonly used media and their influence on embryonic development. Potential ways to improve the production of sheep embryos in vitro with the determination of morphological evaluation of categories of oocytes, methods of synchronization of their maturation in vitro are also highlighted. At the same time, literature data on the synchronization of oocyte-cumulus complexes with the use of a large number of inhibitors of meiotic division are presented, which according to many scientists may be a key factor in improving the efficiency of sheep embryo production in vitro. In addition, the results of studies of many scientists on the expansion of the fertile gap of oocytes of sheep cultured in vitro using certain biologically active substances were analyzed. In conclusion, the prospect of using the technology of in vitro production of sheep embryos in biomedical research is highlighted.

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“…While SFAs, PA and SA, have negative effects on fertilization and preimplantation embryo development, the MUFAs OA exerts positive effects ( Aardema et al , 2011 ; McKeegan and Sturmey, 2011 ; Yang et al , 2017 ). Controversy exists regarding the role of LA in preimplantation embryo development, with positive effects in humans ( Shaaker et al , 2012 ) and negative effects in ovine and bovine models ( Renaville et al , 2010 ; Amini et al , 2016 ; Roura et al , 2018 ). The PUFA α-linolenic acids (ALAs) exerts damaging effects on the reproductive system and it decreases the chance of pregnancy after IVF in humans ( Jungheim et al , 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Embryonic fatty acid metabolism in diabetic pregnancy: the difference between embryoblasts and trophoblasts

Schindler

Dannenberger

Nuernberg

et al. 2020

Molecular Human Reproduction

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During the first days of development the preimplantation embryo is supplied with nutrients from the surrounding milieu. Maternal diabetes mellitus affects the uterine microenvironment, leading to a metabolic adaptation processes in the embryo. We analysed embryonic fatty acid (FA) profiles and expression of processing genes in rabbit blastocysts, separately in embryoblasts and trophoblasts, to determine the potential consequences of maternal diabetes mellitus on intracellular FA metabolism. Insulin-dependent diabetes was induced by alloxan in female rabbits. On day 6 post coitum, FA profiles in blastocysts (embryoblast, trophoblast and blastocoel fluid) and maternal blood were analysed by gas chromatography. The expression levels of molecules involved in FA elongation (fatty acid elongases, ELOVLs) and desaturation (fatty acid desaturases, FADSs) were measured in embryoblast and trophoblast. Maternal diabetes mellitus influenced the FA profile in maternal plasma and blastocysts. Independent from metabolic changes, rabbit blastocysts contained a higher level of saturated fatty acids (SFAs) and a lower level of polyunsaturated fatty acids (PUFAs) compared to the FA profile of the maternal plasma. Furthermore, the FA profile was altered in the embryoblast and trophoblast, differently. While SFAs (palmitic and stearic acid) were elevated in embryoblast of diabetic rabbits, PUFAs, such as docosahexaenoic acid, were decreased. In contrast, in the trophoblast, lower levels of SFAs and higher levels of oleic acid were observed. Embryoblast and trophoblast specific alterations in gene expression were found for ELOVLs and FADSs, key enzymes for FA elongation and desaturation. In conclusion, maternal diabetes mellitus alters embryonic FA metabolism differently in embryoblast and trophoblast, indicating a lineage-specific metabolic adaptive response.

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Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine

Cited by 15 publications

References 32 publications

Advances in in vitro production of sheep embryos

Advances in in vitro production of sheep embryos

Modern achievements of the development of scientific research at obtaining in vitro sheep embryos

Embryonic fatty acid metabolism in diabetic pregnancy: the difference between embryoblasts and trophoblasts

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