The purpose of this search was to investigate the influence of “Thireomagnile” and “Thyrioton” on the antioxidant status of cows in the development of endotoxicosis. The experimental part of the work was performed at the Department of Pharmacology and Toxicology of Stepan Gzhytskyj Lviv National University of Veterinary Medicine and Biotechnologies, in the laboratory of Pharmacology and Toxicology of the State Scientific Research Institute of Veterinary Preparation and Feed Additives (Lviv) and in the Ltd. “Barkom”, village Yampil of Pustomyty district, Lviv region. To solve the tasks of the research, three experimental groups of 10 pregnant cows of the Ukrainian Black-Spotted Dairy breed were formed in each: control and two experimental. Cows of the control group (K) had characteristic clinical signs of endotoxicosis. Animals of the first experimental group (E1) were given 10 mL per animal of “Thyroid magnet” at the eighth and ninth months of gestation. The animals of the second experimental group (E2) were given Thyrioton at a dose of 10 mL per animal in the eighth and ninth months of gestation. As a result of the conducted researches it is found out that in pregnant cows with clinical manifestation of endotoxicosis the inhibition of the activity of the antioxidant protection system and intensification of processes of lipid peroxidation occurs. The use of “Thireomagnile” and “Thyrioton” in cows by the development of endotoxicosis contributed to the increased activity of the antioxidant system, which is indicated by an increase in the activity of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase. The use of of “Thireomagnile” and “Thyrioton” to cows in the development of endotoxicosis contributed to the suppression of lipid peroxidation processes, which is indicated by the low level of primary and final products of lipid peroxidation, namely: lipid hydroperoxides and TBA-active products. Better normalizing action on the equilibrium on the activity of the antioxidant system and the intensity of lipid peroxidation was shown by the introduction of “Thyroid magnet” at a dose of 10 cm3 per animal.

In the conditions of modern man-caused pollution of the environment, environmental problems, as well as improving the quality of livestock products and their food safety, are important and relevant issues today. The study aimed to investigate the effect of the “Metisevit Plus” feed additive on bull blood's morphological and biochemical parameters under lead-cadmium loading conditions. The research was conducted based on the agricultural private enterprise “Ukraine” of Dubrovytsia district of Rivne region on 12 bulls of six months of age, Ukrainian black-and-white dairy breed, which was formed into two groups of 6 animals each. The bulls of the control group were on a standard diet. The bulls of the experimental group were fed the feed additive “Metisevit Plus” at a dose of 0.5 g/kg of feed. This farm has a high content of lead and cadmium in feed. According to the results of experimental studies, it was found that the feed additive “Metisevit Plus” is effective under lead-cadmium load in bulls. Administration of this feed additive to experimental animals helps restore their suppressed hematopoietic function; the number of erythrocytes and hemoglobin in their blood increased by 25.3 and 19.4 %, and the number of leukocytes decreased by 12.4 %, respectively. Metisevit Plus feed additive also enhanced the functional state and protein-synthesizing function of the liver of bulls under artificial conditions. When feeding the feed additive “Metisevit Plus”, a decrease in the activity of both alanine aminotransferase and aspartate aminotransferase in the serum of bulls of the experimental group was found. On day 40 of the experiment, it was found that the activity of alanine and aspartate aminotransferase in the serum of bulls of the experimental group fluctuated within physiological values. When a “Metisevit-Plus” feed additive is added to the diet, there is a tendency to increase the total protein level in bulls of the experimental group. In the study of the albumin level in the blood of bulls of the experimental group, its probable increase was found starting from the 10th day of the experiment. On the 30th and 40th day of the experiment, the albumin level in the blood of bulls in the experimental group was the highest, whereas compared to the control group, it increased by 15.4 and 17.0 %, respectively. Our studies confirm the feasibility of using the feed additive “Metisevit Plus” to prevent lead-cadmium toxicosis.

The evaluation of animals by exteriors and constitutions is an important component in an integrated selection system. Exterior of farm animals is an external manifestation of the constitution and fully characterizes their tribal, productive and adaptive capabilities. The exteriors also assess the degree of animal typical for the line, family, because the type of body structure, along with the indicators of milk productivity is the main selection criterion for the improvement of any breed. The research was carried out on a herd of cows of Ukrainian Black-and- Spotted Dairy breed in the amount of 320 heads in the LTD “Pershe Travnya” Drohobych district Lviv region. The definition of productive types was carried out in accordance with the recommendations from the evaluation of the type of body tissue of dairy cattle of Derzhagroprom of Ukraine (1991). In the result of the evaluation, two groups of animals were identified: milk and milk and meat types. At the same time, in each production type, two groups of cows were established by the method of genealogical analysis: I – up to 75% and II – over 75% of the inheritance of Holstein breed. As a result of the conducted research, it was established that the cows of the Ukrainian Black-and-Spotted Dairy breed are characterized by strong body structure and good muscle development. The assessment of exterior – constitutional parameters of experimental animals revealed, that from 320 cows to dairy type belong 170 heads, or 55.6%, and to a milk-meat type – 142 heads, or 44.4%. It was installed that the cows of Ukrainian Black-and-Spotted Dairy breed of various types of production, despite the same conditions of keeping, are distinguished by the following measurements: height at the withers – 132.9 – 136.2 cm, height in the back - 135.8 – 138.9 cm, height in the sacrum – 137.7 – 140.7 cm, depth of chest – 72.9 – 75.9 cm, breast width 43.4 – 45.9 cm, breast circumference – 193.3 – 196.4 cm, curvature of the body – 160.7 – 165.3 cm, spit curve length of the back – 53.6 – 55.1 cm, girth heel – 19.7 – 20.5 cm. The indicated measurements in the section of lactation in high-clover cows compared to animals with a proportion of heredity of holstein to 75% are generally higher in both types of production. This indicates that with the increase in genotypes the share of heredity and among the animals of the milk-meat type are formed externality signs with a bias in the milk type.

The results of researches in modern herds of meat breeds – classical British, Aberdeen-Angus and autochthonous gray Ukrainian for conducting of pollination in research farms “Polivanivka” of the Institute of Agriculture of Steppe Regions of NAAS of Ukraine and “Progress” of Chernihiv Institute of Agro-Industrial Production in 2009–2012. The ethological behavior of mothers (cows) and their calves was studied in the conditions of keeping and growing livestock on the technology of meat cattle breeding. Animals for conducting research were selected taking into account age, body weight of cows, sex calves, body weight of calves at birth. Collection of accounting materials was carried out according to the data of the primary technological and pedigree records, artificial insemination magazines, materials of ethological observations. Observations were conducted in accordance with the task of preserving genetic diversity in gene pool herds of Aberdeen-Angus and Gray Ukrainian breeds. The herds of animals in the experimental farms kept the meat keeping technology adopted by the farms. The feeding of breeding stock of animals of these breeds was carried out in accordance with established and accepted at experimental farms rations, which consisted of the presence and number of harvested at farms feed. Obtained materials indicate the plasticity and the ability to control the system reproduction reproductive population in modern economic conditions farms. Attention is paid not only to purely selective approaches, but also an attempt is made to substantiate the necessity of adapting breeding breeds to modern systems of economic relations. Knowledge of the ethology of Aberdin-Angus and gray Ukrainian breeds in the conditions of the forest-steppe and steppe zones of Ukraine will allow controlling the processes of reproduction of mother and repair animals in livestock breeding herds, as well as the conservation of genetic resources of aboriginal breeds.

The article presents the results of determining the ability of enterococci extracted from traditional Carpathian cheese bryndza to produce biologically active substances, in particular, amino acids, B vitamins and cations (ammonium, potassium, sodium, magnesium, calcium). It was found that the studied strains of enterococci in different quantities synthesized both essential and essential amino acids. Thus, the essential amino acid lysine was found in the cultivation of strains of E. durans SB18, E. durans SB20, in particular, its concentration was significantly increased by 15.6 and 10.4 %, respectively (P < 0.05) compared to the control. A probable increase in the essential amino acid histidine by 20 and 53.3 % (P < 0.05) was detected in the cultivation of only E. faecium SB12 and E. durans SB18. In addition, it was found a probable increase in threonine synthesis by enterococci E. durans SB6 and E. durans SB18, respectively – 33.3 and 39.6 % (P < 0.05). The replacement amino acid serine was able to synthesize strains of E. faecium SB12, E. durans SB18 and E. durans SB20, while its concentration increased by 40.0 (P < 0.001), 30.0 and 35.0 %, respectively < 0.01), and strains of E. durans, SB6, and E. durans SB18 synthesized glycine, the concentration of which increased by – 10.2 and 16.2 %, respectively (P < 0.01). In addition, it was found that the studied strains in small quantities synthesized B vitamins, or not synthesized at all. In all experimental samples the most vitamin B1 was detected, its concentration increased from 8.5 to 10.0 times (P < 0.001). Riboflavin was synthesized by three strains of enterococci – E. durans SB6, E. durans SB18, E. durans SB20, so the concentration of vitamin B2 probably increased, respectively, 4.1, 2.0 and 2.0 times (P < 0.05). Enterococci E. durans SB6, E. faecium SB12, E. durans SB18 and E. durans SB20 synthesized in significant quantities only vitamin B3, in particular, its concentration probably increased by 1.5, 1.5 (P < 0.05), respectively, 1.5 (P < 0.01) and 1.6 (P < 0.001) times, and vitamin B5 was produced by E. faecium SB12, E. durans SB18 and E. durans SB20, the concentration of nicotinic acid increased, respectively, 2.9 (P < 0.05), 8.4 and 9.5 (P < 0.001) times. Analysis of the macroelement composition of the supernatant of enterococci showed that strains of E. durans, SB6, E. faecium SB12, E. durans SB18 and E. durans SB20 are able to produce only Calcium, in particular, found a probable increase, respectively, in 1.8, 2.4, 1.6 and 1.4 times (P < 0.05).