Effect of low density lipoproteins in extender on freezability and fertility of buffalo (Bubalus bubalis) bull semen

Akhter, Shamim; Ansari, Muhammad Tayyab; Rakha, Bushra Allah; Andrabi, S. M. H.; Khalid, M.; Ullah, Nemat

doi:10.1016/j.theriogenology.2011.04.009

Cited by 35 publications

(42 citation statements)

References 19 publications

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“…This determines that outcomes can be improved by adding extracted LDL to the extender rather than whole egg yolk. Our results are consistent with those obtained in bull (Moussa et al, 2002;Vera Munoz et al, 2009;Amirat-Briand et al, 2004Hu et al, 2010Hu et al, , 2011, boar (Hu et al, 2006(Hu et al, , 2008Jiang et al, 2007), dog (Bencharif et al, 2008, buffalo (Akhter et al, 2011), ram (Moustacas et al, 2011) and horse (Moreno et al, 2013). Low-density lipoproteins (0.982 g/mL) appear in the soluble fraction of egg yolk called plasma.…”

Section: Discussionsupporting

confidence: 93%

“…Thus, the concentration of LDL that offered the best protection of 10% (w/v) is consistent with the findings of Akhter et al (2011) in buffalo, while concentrations of 9% and 8%, respectively have been reported for boar (Hu et al, 2006) and bull (Moussa et al, 2002;Hu et al, 2011). The optimal LDL concentration therefore seems to be related to the animal species.…”

Section: Discussionsupporting

confidence: 87%

“…However, its protective action is largely thought to be due to low-density lipoproteins (LDL), as the main constituents of egg yolk (2/3 of dry yolk matter). Many investigations have confirmed these findings in bull (Hu et al, 2011), boar (Hu et al, 2006), dog (Bencharif et al, 2008), buffalo (Akhter et al, 2011), ram (Moustacas et al, 2011) and horse (Moreno et al, 2013). However, to the best of our knowledge the use of LDL as a non-permeable CPA has not yet been tested in the rabbit.…”

Section: Introductionmentioning

confidence: 87%

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Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Iaffaldano¹,

Iorio²,

Rosato³

et al. 2014

Animal Reproduction Science

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 87%

See 1 more Smart Citation

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Iaffaldano¹,

Iorio²,

Rosato³

et al. 2014

Animal Reproduction Science

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“…The delivery rate was 46.67% in TEY20 versus 40% in CEY20 in this study. The conception rate in river buffalo using post-thawed ejaculate sperm in different extenders was reported in widely range, i.e 44% in tris-egg yolk and 47% in Bioexcell (soya milk-based) extenders (Akhter et al, 2010), 41.5% in egg yolk-based and 56% in soya bean-based extenders (Akhter et al, 2011a), 41.5% in egg yolk-based and 53.5% in LDLs-based extenders (Akhter et al, 2011b).…”

Section: Resultsmentioning

confidence: 99%

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Yulnawati¹,

Maheshwari²,

Rizal³

et al. 2013

Med Pet

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Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo', as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity. Key words: artificial insemination, epididymal sperm, spotted buffalo ABSTRAKKerbau belang merupakan jenis kerbau unik yang ditemukan di Tana Toraja, Sulawesi Selatan. Populasi ternak ini menurun secara drastis karena tingginya laju pemotongan pada upacara adat Rambu Solo', serta keyakinan dalam adat yang tidak memperbolehkan kerbau belang jantan melakukan aktivitas reproduksi. Berdasarkan hal tersebut, studi ini dilakukan sebagai langkah awal proses konservasi dengan menggunakan cauda epididimis sebagai sumber sperma potensial. Studi ini menggunakan dua jenis bufer dalam bahan pengencer berbasis kuning telur, yaitu tris hidroxil amino metana (TEY20) dan trisodium sitrat dehidrat (CEY20), untuk menguji kualitas dan daya fertilitas sperma pasca-thawing dalam proses inseminasi buatan. Hasil yang diperoleh menunjukkan bahwa persentase motilitas progresif adalah sebesar 40% dan 39.17%, dengan persentase hidup mati sebesar 65.99% dan 63.26%, serta persentase membran plasma utuh sebesar 65.43% dan 63.03% dalam bahan pengencer TEY20 dan CEY20 secara berturut-turut. Dapat disimpulkan bahwa bahan pengencer berbasis tris dan sitrat memiliki kemampuan yang sama dalam mempertahankan kualitas dan daya fertilitas sperma epididimis kerbau belang pasca-thawing.

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“…The basic ingredients of current sperm freezing extenders are the same as those used 35 years ago, although in recent years several authors have investigated the incorporation of new compounds to the freezing extender, like low-density lipoproteins (LDL) [2,3], different nonpermeating sugars [4,5], antioxidants [6,7] showing that it is possible to improve the post-thaw sperm quality modifying the actual cryopreservation extenders. Among one of these new compounds that could improve the quality of frozen semen is the ethylene glycol tetraacetic acid (EGTA).…”

Section: Introductionmentioning

confidence: 99%

Effect of the EGTA (Ethylene Glycol Tetraacetic Acid) Supplementation in the Freezing Extender on Quality of Cryopreserved Bull Sperm

Posado¹,

Jj²,

Gómez-Fernández³

et al. 2017

Int J Vet Sci Res

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ContentsThe aim of this study was to evaluate the effect of the inclusion of divalent ion chelating agent EGTA (ethylene glycol tetraacetic acid) in the freezing extender on quality of cryopreserved bull sperm. The freezing extender (egg yolk Tris glycerol) was supplemented with 0 (non-supplemented), 1, 5, 10 and 15 mM EGTA. Bull sperm EGTA improved post thaw sperm quality but in different parameters according to the concentration of EGTA, 5 mM improved the acrosomal state and 1 mM the motility. Our results suggest the EGTA supplementation in the freezing extender of bull sperm might improve the post-thawing sperm quality.

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Effect of low density lipoproteins in extender on freezability and fertility of buffalo (Bubalus bubalis) bull semen

Cited by 35 publications

References 19 publications

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Effect of the EGTA (Ethylene Glycol Tetraacetic Acid) Supplementation in the Freezing Extender on Quality of Cryopreserved Bull Sperm

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