Effect of low‐level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5‐fluorouracil chemotherapy

Theodoro, Letícia Helena; Longo, Mariéllen; Ervolino, Edílson; Duque, Cristiane; Ferro-Alves, Marcio Luiz; Assem, Naida Zanini; Louzada, Lidiane Mendes; Garcia, Valdir Gouveia

doi:10.1111/jre.12347

Cited by 21 publications

(42 citation statements)

References 49 publications

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“…Even aggravation of EP in animals receiving 5‐FU had already been shown (Garcia et al., ; Theodoro et al., , ), the capacity of both CIS and 5‐FU to alter hosts’ immune and inflammatory response leading to bone loss in the initial periods of 3 and 5 days was firstly assessed by the present study. Additional studies are needed to better understand the effects and mechanisms of action of 5‐FU and CIS on periodontal tissues.…”

Section: Discussionmentioning

confidence: 56%

“…Both injections of PSS were 0.5 ml. The doses of the first and the second injections of 5‐FU were 80 and 40 mg/kg of body weight, respectively (Theodoro et al., ), and for CIS were 5 and 2.5 mg/kg of body weight, respectively (Matheus et al., ). For both antineoplastic agents, the dosages administered were based on a protocol utilized for oncological therapy in humans which uses body surface area in their determination (Du Bois & Du Bois, ; Food and Drug Administration, ).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, it would be of great interest to better understand the biological mechanisms by which 5‐FU increased the severity of EP observed in previous studies (Garcia et al., ; Theodoro et al., ). Also, considering the frequent use of CIS in chemotherapy, it would be very important to assess if this drug had any effect on periodontal tissues.…”

Section: Introductionmentioning

confidence: 96%

“…() and Theodoro et al. (, ). They concluded that 5‐FU exacerbated the inflammatory response and increased the severity of EP at 14, 22 and 37 days after its induction in rats.…”

Section: Introductionmentioning

confidence: 98%

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Antineoplastic agents exacerbate periodontal inflammation and aggravate experimental periodontitis

Gusman

Ervolino

Theodoro

et al. 2019

J Clinic Periodontology

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Aim This study evaluated the effects of 5‐fluorouracil (5‐FU) and cisplatin (CIS) in healthy periodontal tissues and in the early stages of experimental periodontitis (EP) in rats. Methods One hundred and eighty male rats were divided into three groups, which were submitted to the following systemic treatments: physiological saline solution (PSS); CIS and 5FU. Each group was subdivided into two subgroups: without (NEP) and with (EP) induction of EP. Animals were euthanized at 3, 5 and 7 days post‐treatment. Histological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (for tumour necrosis factor‐α, interleukin‐1β and receptor activator of nuclear factor‐κB ligand) analyses were performed. Data were statistically analysed. Results CIS‐NEP and 5FU‐NEP showed more inflammation than PSS‐NEP at 3, 5 and 7 days. CIS‐EP and 5FU‐EP showed more inflammation and lower PBF than PSS‐EP at all periods of evaluation. 5FU‐EP showed lower PBF than CIS‐EP at 5 and 7 days. Conclusion 5‐FU and CIS exacerbated periodontal inflammation and aggravated the progression of EP in its early stages.

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Section: Discussionmentioning

confidence: 56%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

“…() and Theodoro et al. (, ). They concluded that 5‐FU exacerbated the inflammatory response and increased the severity of EP at 14, 22 and 37 days after its induction in rats.…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Antineoplastic agents exacerbate periodontal inflammation and aggravate experimental periodontitis

Gusman

Ervolino

Theodoro

et al. 2019

J Clinic Periodontology

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“…During such treatment, it is common for changes in the oral cavity to occur: such changes include mucositis, ulceration, gingival bleeding [2],degeneration in oral keratinocytes [3] and altered collagen synthesis [4], as well as changes in connective tissue and the oral microbial environment [5]. Furthermore there is increased destruction of periodontal tissues [6,7] with progressive periodontal attachment loss [8], increased production of proinflammatory cytokines [9] and the remodelling ability of bone and soft tissue is compromised [10].…”

Section: Introductionmentioning

confidence: 99%

Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: histomorphometrical, immunohistochemical, immunological and microbiological analyses

Longo

Garcia

Ervolino

et al. 2019

Photodiagnosis and Photodynamic Therapy

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Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: Histomorphometrical, Immunohistochemical, Imunological and Microbiological Analyses. http://researchonline.ljmu.ac.uk/id/eprint/9722/ Article LJMU has developed LJMU Research Online for users to access the research output of the University more effectively. , LH (2018) Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: Histomorphometrical, Immunohistochemical, Imunological and Microbiological Analyses. Abstract Background: The aim of this study was to evaluate the effect of multiple sessions of antimicrobial photodynamic therapy (aPDT) on the treatment of experimental periodontitis (EP) in rats treated with chemotherapy. Methods: Chemotherapy using 5-fluorouracil (5-FU) consisted of intraperitoneal administration of 60 and 40 mg/kg of 5-FU. 120 rats were subjected to chemotherapy with 5-FU and divided into groups: PT (periodontal treatment); PT+1aPDT (PT and single aPDT session); PT+4aPDT(PT and 4 sessions of aPDT); 1aPDT (single aPDT session); 4aPDT(4 sessions of aPDT). EP was induced in the mandibular molars via ligature placement. The alveolar bone loss (ABL) area in the furcation region was analysed histometrically. Proliferating cell nuclear antigen (PCNA), tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) and cleaved caspase-3 (CC3) were analysed by immunohistochemistry. Prostaglandin E2 was quantified using an ELISA, tumor necrosis factor (TNF)-α and interleukin (IL)-6 were assessed using a multiplex method. The prevalence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia and Fusobacterium nucleatum was assessed using PCR. The data were statistically analysed (P<0.05).Results: The PT+4aPDT group showed lower ABL than the PT or 1aPDT groups on day 7.Rats treated with aPDT showed a higher number of PCNA-positive cells with reduced immunolabeling of RANKL. Significant reductions in Prevotella nigrescens were observed in the PT+4aPDT group and in Aggregatibacter actinomycetemcomitans for the 1aPDT and 4aPDT groups.Conclusion: Repeated sessions of aPDT as an adjunct or alternative therapy were effective at reducing ABL, regulating bone metabolism, and reducing Prevotella nigrescens and Aggregatibacter actinomycetemcomitans.

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Impact of Bifidobacterium animalis subsp. lactis HN019 probiotic in the prevention of periodontitis associated with immunosuppression

Prado

Levi

Silva

et al. 2022

Journal of Periodontology

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Background This study evaluated the effects of the probiotic Bifidobacterium animalis subsp. lactis HN019 (HN019) in the development of experimental periodontitis (EP) in rats submitted to chemotherapy (5‐fluorouracil [5FU]). Methods Eighty male rats were divided into the following groups: control (C); treated with 5FU (60 mg/kg at day 30 and 40 mg/kg at day 32); treated with probiotic (HN019) (daily, for 44 days, starting at day 0); treatment with 5FU and probiotic (5FU‐HN019); only EP (EP) (ligature placed on lower first molars at day 30, maintained for 14 days); EP and treatment with 5FU (EP‐5FU); EP and treatment with probiotic (EP‐HN019); and EP and treatment with 5FU and probiotic (EP‐5FU‐HN019). Euthanasia occurred at day 44. Morphometric, histomorphometric, microtomographic, immunohistochemical, immunoenzymatic, and gene expressions analyses were performed. The data obtained were statistically analyzed (p < 0.05). Results The EP‐5FU‐HN019 group showed less bone and connective tissue loss when compared with EP‐5FU group, while EP‐HN019 and EP‐5FU‐HN019 groups had greater bone volume than EP and EP‐5FU groups, respectively (p < 0.05). A decrease in immunostaining for tartrate‐resistant acid phosphatase and RANKL, an increase for osteoprotegerin and lower interleukin‐1β levels were observed in EP‐5FU‐HN019 group, when compared with EP‐5FU group (p < 0.0001). Probiotic therapy led to an increase in the proportions of B. lactis in the feces (p = 0.0018), but not in the biofilm, and reduced the expression of Fusobacterium nucleatum and Prevotella intermedia in the biofilm (p < 0.0001). Conclusion B. lactis HN019 reduced the severity of EP in rats submitted to chemotherapy, modulating immunoinflammatory parameters in periodontal tissues and reducing periodontopathogens expression on biofilm in rats submitted to chemotherapy.

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Effect of low‐level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5‐fluorouracil chemotherapy

Abstract: Treatment with 5-FU worsened EP, and multiple LLLT sessions adjuvant to SRP seemed to improve periodontitis in rats subjected to 5-FU chemotherapy.

Cited by 21 publications

References 49 publications

Antineoplastic agents exacerbate periodontal inflammation and aggravate experimental periodontitis

Antineoplastic agents exacerbate periodontal inflammation and aggravate experimental periodontitis

Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: histomorphometrical, immunohistochemical, immunological and microbiological analyses

Impact of Bifidobacterium animalis subsp. lactis HN019 probiotic in the prevention of periodontitis associated with immunosuppression

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