Effect of low temperature on the activity of entomopathogenic nematodes

Sharmila, R.; Subramanian, Sevgan

doi:10.15740/has/ijfci/7.1/19-23

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Yadav (2016) also reported that the storage of the IJs of the same EPN species at a lower temperature (5 °C) led to a high decrease in the survival of IJs and S. glaseri was the only EPN species that survived after 4 months of storage at 5 °C. Similar to our findings, Sharmila and Subramanian (2016) reported 50 and 48% survival rates in the IJs of H. indica that were stored in deionized water for three months at 10 and 25 °C, respectively. However, in the current study, all tested EPN species survived for 6 months albeit with a notable decrease in survival rates of IJs.…”

Section: Pathogenicity Of Infective Juvenilessupporting

confidence: 91%

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

AKI,

YÜKSEL,

İMREN

et al. 2023

Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi

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Entomopathogenic nematodes (EPNs) (Rhabditida: Heterorhabditidae and Steinernematidae) are effective biocontrol agents for many insect pests and are generally stored for a period of time prior to their use in the laboratory or field. However, unfavorable storage conditions have a great impact on the survival and infectivity of EPNs. This study was conducted to determine the optimum storage conditions of infective juveniles (IJs) of four native EPN species (Heterorhabditis bacteriophora FLH-4H, H. indica 216-H, Steinernema feltiae KCS-S, and S. bicornotum MGZ-4S) under laboratory conditions. The survival capability of the IJs was tested at different concentrations (500, 1000, 1500, and 2000 IJs), temperatures (9 and 25 °C) and storage media [double-distilled water (ddH2O), tap water, and sterile Ringer solution]. In general, the survival of IJs of tested EPN species was the highest at the 1st month after treatment (MAT) at the concentrations of 1000 and 1500 IJs and gradually decreased with the increasing storage periods. The survival rates of the IJs of Steirnematids were generally higher than Heterorhabditid species. The highest survival of IJs was generally obtained after 1-month storage in Ringer solution at 9°C while tap water led to poor survival for the IJs at both temperatures tested. The IJs that were stored at 9°C induced higher mortalities on the larvae of Galleria mellonella (L.) (Lepidoptera: Pyralidae). The results showed that the IJs of tested EPN species can remain viable for a longer period of time in Ringer solution at 1000 and 1500 IJs concentrations at 9°C without losing much of their infectivity.

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Section: Pathogenicity Of Infective Juvenilessupporting

confidence: 91%

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

AKI,

YÜKSEL,

İMREN

et al. 2023

Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi

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“…In agricultural use, it is not uncommon for EPNs to endure extended storage times. It has been previously demonstrated that storage temperature may affect both the survival and infectivity of EPNs [ 23 , 24 ]. Since previous work had demonstrated that the response of some EPNs to prenol were affected by moderate storage times [ 5 , 18 ], we wondered how longer time periods of storage/aging would impact the IJs’ behavior.…”

Section: Resultsmentioning

confidence: 99%

EPNs Exhibit Repulsion to Prenol in Pluronic Gel Assays

Baiocchi

Dillman

2020

Insects

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Entomopathogenic nematodes (EPNs) are lethal parasites of insects that have become valuable in biological control and as a model system for studying host–parasite interactions, behavioral ecology, neurobiology, and genomics, among other fields. Their ability to locate hosts is paramount to successful infection and host seeking has been extensively studied in many species in the lab. Here, we explored the usefulness of pluronic gel as a medium to assess EPN host seeking in the lab by characterizing the response of Steinernema carpocapsae, S. feltiae, S. glaseri, S. riobrave, Heterorhabditis bacteriophora, and H. indica to the odor prenol. We found that the infective juveniles (IJs) of these species were repelled by prenol in pluronic gel. We then evaluated how storing the IJs of S. carpocapsae, S. feltiae, and S. glaseri for different amounts of time affected their behavioral responses to prenol. The response of S. carpocapsae was significantly affected by the storage time, while the responses of S. feltiae and S. glaseri were unaffected. Our data support the notion that pluronic gel is a useful medium for studying EPN behavior and that the response of S. carpocapsae to informative odors is significantly affected by long-term storage.

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Biocontrol Potential of Turkish Entomopathogenic Nematodes Against the Citrus Mealybug, Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) Under Laboratory Conditions

NAJM¹,

Yüksel

Canhilal

2023

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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The citrus mealybug, Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) is one of the major pests of Citrus orchards in Turkey. Management of P. citri is quite challenging due to its cryptic and polyphagous feeding behavior. In the current study, the control potential of native entomopathogenic nematodes (EPNs) species (Heterorhabditis indica 216-H, H. bacteriophora FLH-4H, Steinernema carpocapsae E-76, S. feltiae KCS-4S, and S. bicornotum MGZ-4S) against P. citri was evaluated under laboratory conditions at different concentrations (80, 100, 150, 200 IJs Adult-1) and temperatures (20, 25, 30 ºC). The mortality rates ranged between 16 and 58% at the highest concentration 48 hours after treatment. The highest efficacy (68%) was obtained by Heterorhabditis indica 216-H at the highest concentration at 25 ºC. The mortality rates were generally higher at 25 ºC than other temperatures tested and H. indica 216-H performed better than other EPN species tested at this temperature at all concentrations. The results indicate that H. indica 216-H have a great potential in the control of P. citri.

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Effect of low temperature on the activity of entomopathogenic nematodes

Cited by 3 publications

References 10 publications

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

EPNs Exhibit Repulsion to Prenol in Pluronic Gel Assays

Biocontrol Potential of Turkish Entomopathogenic Nematodes Against the Citrus Mealybug, Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) Under Laboratory Conditions

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