Effect of <i>Bacillus amyloliquefaciens</i>-based Direct-fed Microbial on Performance, Nutrient Utilization, Intestinal Morphology and Cecal Microflora in Broiler Chickens

Abstract

The present study was conducted to evaluate the effect of the dietary supplementation of Bacillus amyloliquefaciens-based direct-fed microbial (DFM) on growth performance, nutrient utilization, intestinal morphology and cecal microflora in broiler chickens. A total of two hundred and eighty eight 1-d-old Arbor Acres male broilers were randomly allocated to one of four experimental treatments in a completely randomized design. Each treatment was fed to eight replicate cages, with nine birds per cage. Dietary treatments were composed of an antibiotic-free basal diet (control), and the basal diet supplemented with either 15 mg/kg of virginiamycin as antibiotic growth promoter (AGP), 30 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 30) or 60 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 60). Experimental diets were fed in two phases: starter (d 1 to 21) and finisher (d 22 to 42). Growth performance, nutrient utilization, morphological parameters of the small intestine and cecal microbial populations were measured at the end of the starter (d 21) and finisher (d 42) phases. During the starter phase, DFM and virginiamycin supplementation improved the feed conversion ratio (FCR; p<0.01) compared with the control group. For the finisher phase and the overall experiment (d 1 to 42) broilers fed diets with the DFM had better body weight gain (BWG) and FCR than that of control (p<0.05). Supplementation of virginiamycin and DFM significantly increased the total tract apparent digestibility of crude protein (CP), dry matter (DM) and gross energy during both starter and finisher phases (p<0.05) compared with the control group. On d 21, villus height, crypt depth and villus height to crypt depth ratio of duodenum, jejunum, and ileum were significantly increased for the birds fed with the DFM diets as compared with the control group (p<0.05). The DFM 30, DFM 60, and AGP groups decreased the Escherichia coli population in cecum at d 21 and d 42 compared with control group (p<0.01). In addition, the population of Lactobacillus was increased in DFM 30 and DFM 60 groups as compared with control and AGP groups (p<0.01). It can be concluded that Bacillus amyloliquefaciens-based DFM could be an alternative to the use of AGPs in broilers diets based on plant protein.

Bacillus Coagulans Enhance the Immune Function of the Intestinal Mucosa of Yellow Broilers

“…can stimulate the differentiation and proliferation of intestinal epithelial cells and improve nutrient utilization (Artis et al, 2008;Wells et al, 2011;Duerr et al, 2012). Lei reported that broilers receiving a direct-fed microbial based on Bacillus amyloliquefaciens significantly increased crypt depth and villus height to crypt depth ratio in the duodenum, jejunum, and ileum (Lei et al, 2015). Similarly, in broilers fed diets with 200 mg/kg Bacillus coagulans, an increase in V/C ratio was also observed (Lin et al, 2014).…”

“…Lei reported that the dietary inclusion of spores of a lactobacillus improved the conversion ratio and reduced mortality and culling rate of broilers (Lei et al, 2015). Lactobacilli improve calcium and phosphorus utilization, and lactic acid promotes the transformation of pepsinogen into protease, stimulating the peristalsis of the small intestine to enhance nutrient digestion (Ma et al, 2014;Giang et al, 2010).…”

Immune Responses Induced by Recombinant Bacillus Subtilis Expressing the Hemagglutinin Protein of H5N1 in chickens

“…B. subtilis has been used as a feed additive to improve the microorganismal environment in the intestine18. We weighed the chickens after they developed immunity (Fig.…”

Influência da qualidade microbiológica da água de dessedentação na morfologia intestinal de frangos de corte

“…Resultado semelhante também foi observado por Giannenas et al (2010) após adição do cogumelo Agaricus bisporus à dieta que não promoveu efeitos tróficos na mucosa intestinal de frangos de corte, embora tenha favorecido a microbiota intestinal. Já Lei et al (2015) observaram aumento na altura das vilosidades intestinais a partir dos 21 dias de idade ao adicionarem Bacillus amyloliquefaciens à dieta como alternativa ao uso de melhoradores do crescimento na prevenção de enfermidades.…”