Effect of membrane stiffness and cytoskeletal element density on mechanical stimuli within cells: an analysis of the consequences of ageing in cells

Xue, Feng; Lennon, Alex; McKayed, Katey K.; Campbell, Veronica A.; Prendergast, Patrick J.

doi:10.1080/10255842.2013.811234

Cited by 32 publications

(15 citation statements)

References 45 publications

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“…The aging process, although not a disease, also induces changes in the mechanical properties of individual cells, which may contribute, in part, to the degradation of cellular function (54,55). For most cell types, age causes an increase in cell stiffness along with an inability to fully recover www.annualreviews.org • High-Throughput Mechanophenotyping 2.7…”

Section: Effects Of Diseasementioning

confidence: 98%

High-Throughput Assessment of Cellular Mechanical Properties

Darling

Carlo

2015

Annu. Rev. Biomed. Eng.

184

175

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Traditionally, cell analysis has focused on using molecular biomarkers for basic research, cell preparation, and clinical diagnostics; however, new microtechnologies are enabling evaluation of the mechanical properties of cells at throughputs that make them amenable to widespread use. We review the current understanding of how the mechanical characteristics of cells relate to underlying molecular and architectural changes, describe how these changes evolve with cell-state and disease processes, and propose promising biomedical applications that will be facilitated by the increased throughput of mechanical testing: from diagnosing cancer and monitoring immune states to preparing cells for regenerative medicine. We provide background about techniques that laid the groundwork for the quantitative understanding of cell mechanics and discuss current efforts to develop robust techniques for rapid analysis that aim to implement mechanophenotyping as a routine tool in biomedicine. Looking forward, we describe additional milestones that will facilitate broad adoption, as well as new directions not only in mechanically assessing cells but also in perturbing them to passively engineer cell state.

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Section: Effects Of Diseasementioning

confidence: 98%

High-Throughput Assessment of Cellular Mechanical Properties

Darling

Carlo

2015

Annu. Rev. Biomed. Eng.

184

175

View full text Add to dashboard Cite

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“…A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 7 3. Sensing cell damage While they provide valuable insights into the mechanism of cell repair, liposomes and erythrocyte ghosts frequently do not represent the behaviour of many cell types.…”

Section: Spontaneous Resealing Of "Relaxed" Simple Membranesmentioning

confidence: 99%

“…Other components such as plasmalemma shape and composition [7,8], membrane in plane tension [3,7], tether force (i.e. force linked to plasmalemma to cortex attachments) [3],contacts with the extra-cellular matrix (ECM), cell-cell contacts, shear stresses, stretch stresses, or even hydrostatic pressure, may also be involved in cellular tensegrity (reviewed in [6,9]).…”

Section: Introductionmentioning

confidence: 99%

Plasma membrane and cytoskeleton dynamics during single-cell wound healing

Boucher

Mandato

2015

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Wounding leads not only to plasma membrane disruption, but also to compromised cytoskeleton structures. This results not only in unwarranted exchanges between the cytosol and extracellular milieu, but also in loss of tensegrity, which may further endanger the cell. Tensegrity can be described as the interplay between the tensile forces generated by the apparent membrane tension, actomyosin contraction, and the cytoskeletal structures resisting those changes (e.g., microtubules). It is responsible for the structural integrity of the cell and for its ability to sense mechanical signals. Recent reviews dealing with single-cell healing mostly focused on the molecular machineries controlling the traffic and fusion of specific vesicles, or their role in different pathologies. In this review, we aim to take a broader view of the different modes of single cell repair, while focussing on the different ways the changes in plasmalemma surface area and composition, plasmalemma tension, and cytoskeletal dynamics may influence and affect single-cell repair.

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“…e geometric sizes of the cells were determined using AFM topography images because the AFM can provide detailed information about the topography of the cytoplasm membrane [24]. e elastic moduli of the cells at different locations were obtained by fitting force (F)-indentation (δ) data using the Hertzian and Sneddon formula that relates the indentation force and indentation depth, which is expressed as [25]…”

Section: Cell Preparation and Afm Indentationmentioning

confidence: 99%

Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method

Wang

et al. 2019

Computational and Mathematical Methods in Medicine

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The stiffness of cells, especially cancer cells, is a key mechanical property that is closely associated with their biomechanical functions, such as the mechanotransduction and the metastasis mechanisms of cancer cells. In light of the low survival rate of single cells and measurement uncertainty, the finite element method (FEM) was used to quantify the deformations and predict the stiffness of single cells. To study the effect of the cell components on overall stiffness, two new FEM models were proposed based on the atomic force microscopy (AFM) indentation method. The geometric sizes of the FEM models were determined by AFM topography images, and the validity of the FEM models was verified by comparison with experimental data. The effect of the intermediate filaments (IFs) and material properties of the cellular continuum components on the overall stiffness were investigated. The experimental results showed that the stiffness of cancer cells has apparent positional differences. The FEM simulation results show that IFs contribute only slightly to the overall stiffness within 10% strain, although they can transfer forces directly from the membrane to the nucleus. The cytoskeleton (CSK) is the major mechanical component of a cell. Furthermore, parameter studies revealed that the material properties (thickness and elasticity) of the continuum have a significant influence on the overall cellular stiffness while Poisson’s ratio has less of an influence on the overall cellular stiffness. The proposed FEM models can determine the contribution of the major components of the cells to the overall cellular stiffness and provide insights for understanding the response of cells to the external mechanical stimuli and studying the corresponding mechanical mechanisms and cell biomechanics.

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Effect of membrane stiffness and cytoskeletal element density on mechanical stimuli within cells: an analysis of the consequences of ageing in cells

Abstract: Regarding the effect of ageing, the models suggest only small, although possibly physiologically significant, differences in internal biophysical stimuli between normal and aged cells.

Cited by 32 publications

References 45 publications

High-Throughput Assessment of Cellular Mechanical Properties

High-Throughput Assessment of Cellular Mechanical Properties

Plasma membrane and cytoskeleton dynamics during single-cell wound healing

Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method

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