EFFECT OF METHANOL CONCENTRATION AS A SOLVENT ON TOTAL PHENOLIC AND FLAVONOID CONTENT OF BELUNTAS LEAF EXTRACT (Pulchea indica L.)

Mahasuari, Ni Putu Sinta; Paramita, Ni Luh Putu Vidya; Putra, Andhika

doi:10.24843/jpsa.2020.v02.i02.p05

Cited by 14 publications

(11 citation statements)

References 2 publications

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“…Flavonoids are polar compounds that can dissolve into other polar solvents such as ethanol, methanol, butanol, acetone, dimethylformamide, and others [19]. In some studies, organic solvents such as methanol were shown to dissolve flavonoids more effectively than other solvents [20,21].…”

Section: Discussion Phytochemical Active Content Analysis Resultsmentioning

confidence: 99%

Comparison of Phytochemical Content and Antifungal Activity of Bajakah Tampala Stem (Spatholobus Littoralis Hassk.) Methanol and Ethanol Extracts Against Candida Albicans

Putri

Khusmitha

Mahardhika

et al. 2022

a-jhr

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Introduction: Candida albicans causes the second most frequent vaginal infection, vulvovaginal candidiasis (VVC). Bajakah Tampala is a natural plant of Kalimantan that has the potential to be used as a traditional remedy for various diseases. This study aims to determine the phytochemical content and antifungal potential of methanol and ethanol Bajakah Tampala stem extract against the growth of Candida albicans. Methods: Flavonoids, tannin, phenol, and saponin were identified through qualitative analysis of the phytochemical content. Different concentration of Bajakah Tampala methanol and ethanol extract (1.56%, 3.175%, 6.25%, 12.5%, 25%, 50%, and 100%) was tested for antifungal potency using dilution method. The extract that could inhibit the growth of Candida albicans colonies was continued with the concentration density (35%, 37.5%, 40%, 42.5%, 45%, 47.5%, and 50%) in order to determine the Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MKC) by counting the number of colonies. Results: The methanol extract of Bajakah Tampala contains flavonoids, tannins, phenol, and saponin. In contrast, no flavonoids were detected in the ethanol extract. Candida albicans colonies were not inhibited by concentrations of 1.56%, 3.125%, 6.25%, 12.5%, and 25% in ethanol and methanol extracts, respectively. At concentrations of 50% and 100%, methanol extract inhibits colony growth, but ethanol extract is still growing. The antifungal activity of methanol extract showed a MIC of 35% and a MKC of 47.5%, as measured by its concentration density. Conclusion: Flavonoids are present in the Bajakah Tampala methanol extract, which inhibits and kills the growth of Candida albicans but not ethanol.

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Section: Discussion Phytochemical Active Content Analysis Resultsmentioning

confidence: 99%

Comparison of Phytochemical Content and Antifungal Activity of Bajakah Tampala Stem (Spatholobus Littoralis Hassk.) Methanol and Ethanol Extracts Against Candida Albicans

Putri

Khusmitha

Mahardhika

et al. 2022

a-jhr

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“…Pelarut yang memiliki sifat polar hanya dapat mengikat senyawa fitokimia yang juga bersifat polar, sedangkan pelarut yang bersifat non-polar hanya akan berikatan dengan senyawa yang juga bersifat nonpolar. (Mahasuari et al, 2020) bersifat semi-polar juga dapat berikatan dengan senyawa semi-polar hingga non-polar. Pelarut etil asetat diketahui memiliki sifat semi polar, sedangkan pelarut heksan diketahui bersifat non-polar.…”

Section: Pembahasanunclassified

Uji Efektivitas Ekstrak Metanol Buah Pare (Momordica Charantia L.) Terhadap Pertumbuhan Aspergillus Niger

Ilham¹,

Ika²,

Mega³

2022

BIGES JUKES

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Aspergillus niger is a species of fungus from the genus Aspergillus which is reported to be the main cause of otomycosis. Bitter gourd (Momordica charantia L.) contains several compounds, namely phenols, flavonoids, triterpenoids, steroids, saponins, and alkaloids that have potential as antifungals. This study aims to determine the content of phytochemical compounds present in the methanol extract of bitter melon and to test the effectiveness of the methanol extract of bitter melon on the growth of Aspergillus niger through the MIC test. This study used an in vitro laboratory experimental research design with a post-test-only control group design method. The MIC test used the multilevel serial microdilution method which was observed visually. The extract concentrations used were 8000 µg/ml, 4000 µg/ml, 2000 µg/ml, 1000 µg/ml, 500 µg/ml, 250 µg/ml, 125 µg/ml, and 62.5 µg/ml. The fruit samples of this study was bitter gourd obtained from bitter gourd farmers in Bandungan District, Semarang Regency, and pure culture of Aspergillus niger FNCC 6018. Data analysis used descriptive analysis. The results of the phytochemical screening showed positive saponins, flavonoids, and phenolic compounds, and the MIC value was not found. It was concluded that the methanol extract of bitter melon was not effective in inhibiting the growth of Aspergillus niger.

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“…Hasil akhir dari ekstrak kental biji gayam yang diperoleh dibandingkan dengan berat serbuk sebelum dilakukan proses ekstraksi dikalikan 100%.2.3 Pengujian golongan senyawa [10][11][12].…”

Section: Perhitungan Rendemanunclassified

Skrining Senyawa Metabolit Sekunder pada Ekstrak Aseton Biji Gayam (Inocarpus fagifer)

Widayati¹,

Umarudin²

2022

spibio

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Gayam merupakan tumbuhan berkhasiat obat, salah satu bagian yang bisa dimanfaatkan adalah bijinya. Biji gayam memiliki manfaat bagi kesehatan salah satunya sebagai obat pencahar dan diabetes. Tujuan penelitian ini adalah mengetahui hasil identifikasi golongan senyawa metabolit sekunder dari ekstrak biji gayam (Inocarpus fagifer) dengan pelarut aseton. Metode penelitian ini yaitu biji gayam dilakukan determinasi, biji gayam dilakukan proses sortasi basah, pencucian, perajangan, pengeringan, sortasi kering, dan penghalusan. Serbuk kering biji gayam dilakukan ekstraksi metode maserasi selama 3x24 jam. Hasil ekstraksi dilakukan perhitungan nilai rendemen dan skrining fitokimia untuk mengetahui golongan senyawa metabolit sekunder golongan alkaloid, flavonoid, terpenoid, steroid, tanin, dan saponin pada ekstrak biji gayam ditandai positif adanya perubahan warna, endapan, cincin, atau busa. Hasil determinasi tanaman biji gayam memiliki nama ilmiah Inocarpus fagifer. Ekstrak biji gayam diperoleh nilai rendemen sebesar 22%. Hasil skrinning fitokimia senyawa metabolit sekunder positif mengandung senyawa golongan alkaloid, terpenoid, tanin, saponin, dan negatif senyawa golongan flavonoid dan steroid. Temuan ini dapat mendukung klaim secara tradisional/etnomedisinal yang sebelumnya biji gayam dimanfaatkan untuk antidiabetes.

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EFFECT OF METHANOL CONCENTRATION AS A SOLVENT ON TOTAL PHENOLIC AND FLAVONOID CONTENT OF BELUNTAS LEAF EXTRACT (Pulchea indica L.)

Cited by 14 publications

References 2 publications

Comparison of Phytochemical Content and Antifungal Activity of Bajakah Tampala Stem (Spatholobus Littoralis Hassk.) Methanol and Ethanol Extracts Against Candida Albicans

Comparison of Phytochemical Content and Antifungal Activity of Bajakah Tampala Stem (Spatholobus Littoralis Hassk.) Methanol and Ethanol Extracts Against Candida Albicans

Uji Efektivitas Ekstrak Metanol Buah Pare (Momordica Charantia L.) Terhadap Pertumbuhan Aspergillus Niger

Skrining Senyawa Metabolit Sekunder pada Ekstrak Aseton Biji Gayam (Inocarpus fagifer)

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