Effect of methionine and lactic acid bacteria as aflatoxin binder on broiler performance

Istiqomah, L.; Damayanti, Ema; Julendra, Hardi; Suryani, Ade Erma; Sakti, Awistaros Angger; Anggraeni, Ayu

doi:10.1063/1.4985408

Cited by 7 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The study of Ortatatli et al [6] showed that low doses of aflatoxicosis were between 50-100 ppb. The previous study reported that administration of 1% of LAB + 1.2% of methionine in aflatoxincontaminated diet (6.1 ppb) resulted the lowest damage organ (64.4 %) of liver, kidney, spleen, thymus, bursa of Fabricius, pancreas, duodenum and ileal, but did not significantly affect the body weight gain and feed conversion of broiler [20]. The organs damage caused by AF-contaminated diet are hematopoietic organs, Immune system, and reticuloendothelial systems (RES) [21][22][23].…”

Section: Resultsmentioning

confidence: 90%

“…GSH production has homeostatic mechanism in liver, methionine supplemented in feed could not always change the GSH, because production of GSH could not inhibited by cometionine, and its derivates like buthionine and neutral or non polar amino acid (glycine, alanine, phenylalanine, and proline) acidic amino acid (glutamic acid), basic amino acid (lysine), or synthesis (2-amino-2-norboman carboxylic acid and omethilaminoisobutiric acid) [27] and AFB1 bundled by GSH not always in linear curve, on equilibrium point GSH-AFB1 would get weaken [28], that is why 0.8% of methionine could be toxic for liver with the 57.18 ppb of AF, even though that dosage suitable for 1000 ppb (1 ppm) of AF. 1% of LAB could reduce 44.5% of lesions at 21 days and 31.1% of lesions at 35 days for 2.71 ppb of AF [20], could not adequat enough for binding 57.18 ppb of AF. This also applied to a combination between 1% of LAB and 0.8% of methionine could liver toxicity and got necrosis and interfere erytron as a homeostasis of hematological function.…”

Section: Resultsmentioning

confidence: 91%

See 1 more Smart Citation

Aflatoxin effect on erythrocyte profile and histopathology of broilers given different additives

Karimy

Sutrisno²,

Agus³

et al. 2017

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Abstract. The aim of this study was to evaluate erythrocyte profile and microscopic changes effect of AF induces by low level (57.18 ppb) and chronic exposure (34 days) with administration of additive (Lactobacillus plantarum G7 and methionine). Aflatoxin-contaminated corn was prepared by inoculate Aspergillus flavus FNCC 6002 on corn. Total number of 576 broiler Lohman strain (MB202) unsexed DOC were allocated completely randomized into four treatments and 12 replicates, with 12 broiler chicks each. The treatments as follows: T1= aflatoxin-contaminated diet, T2= aflatoxin-contaminated diet + 1% of LAB (w/w), T3= aflatoxin-contaminated diet + 0.8% of methionine (w/w), and T4= aflatoxin-contaminated diet + 1% of LAB + 0.8% of methionine (w/w). The effect of treatments was evaluated using ANOVA and the difference among mean treatments were analyzed using DMRT. The result showed that administration of additives had no significant effect (P>0.05) on erythrocyte profile, liver, and bursa of Fabricius. The dose of additive in each treatment (T2, T3, T4) were insufficient to reduce adverse effect of chronic aflatoxicosis. It was concluded that the LAB dose for binding AF (57.18%) should be evaluated and the dose for methionine should be reduced for chronic treatment of aflatoxicosis.

show abstract

Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 91%

Aflatoxin effect on erythrocyte profile and histopathology of broilers given different additives

Karimy

Sutrisno²,

Agus³

et al. 2017

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Rata-rata diameter zona hambat ekstrak daun kirinyuh (C. odorata) Pada gambar 3 terlihat aktivitas zona hambat setiap perlakuan tidak mengalami peningkatan yang sama. Hal ini sejalan seperti yang diungkapkan oleh Elifah (2010) dalam Istiqomah [5] bahwa zona hambat tidak selalu sebanding dengan naiknya konsentrasi. Hal ini terjadi karena perbedaan kecepatan difusi senyawa antibakteri pada media agar, jenis dan konsentrasi senyawa antibakteri yang berbeda juga memberikan zona hambat yang berbeda pada lama waktu tertentu.…”

Section: Aktivitas Antibakteri Daun Kirinyuhunclassified

AKTIVITAS ANTIBAKTERI DAUN KIRINYUH (Chromolaena odorata (L.) R.M.King & H.Rob.) TERHADAP BAKTERI GANGREN

Yutika

Rusli

Ramadhan

2015

Proceeding of Mulawarman Pharmaceuticals Conferences

View full text Add to dashboard Cite

Chromolaena leaves extract have antibacterial activity. The aim of this research is to determine antibacterial activity of ABSTRAKDaun kirinyuh mempunyai aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun kirinyuh terhadap bakteri luka gangren yang dikultivasi dari nanah luka penderita gangren diabetik. Metode yang digunakan untuk pengujian aktivitas antibakteri adalah metode difusi agar menggunakan paper disk. Konsentrasi ekstrak etanol daun kirinyuh yang digunakan yaitu 5%, 10%, 15%, 20%, 25%, 30%, dan aquades sebagai kontrol negatif. Hasil penelitian menunjukkan adanya aktivitas daya hambat dari ektrak daun kirinyuh tersebut Kategori kekuatan daya hambat dari ekstrak yaitu termasuk kategori sedang (5-10mm). Konsentrasi terbaik terdapat pada konsentrasi 30%.Kata kunci: Chromolaena odorata, bakteri penginfeksi luka gangren, antibakteri. PENDAHULUANDiabetes millitus adalah penyakit metabolik yang ditandai dengan hiperglikemia akibat kerusakan proses sekresi atau insulin, atau keduanya. Organisasi Kesehatan Dunia (WHO) memprediksikan bahwa jumlah pasien diabetes di Indonesia akan meningkat dari 8,4 juta pada 2000 menjadi 21,3 miliar tahun 2030. International Diabetes Federation (IDF) juga memprediksikan kondisi serupa. Prevalensi diabetes mellitus di Indonesia adalah 5,7% di 2010. Diabetes saat ini mempengaruhi lebih dari 194 juta orang di seluruh dunia dan diperkirakan akan mencapai 333 juta pada tahun 2025 yang banyak terjadi di negara-negara berkembang [1] Diabetes mellitus dapat menyebabkan komplikasi Diabetic Foot Ulcer (DFU) atau gangren diabetik. Hal tersebut dikarenakan trauma akibat benda tajam yang tidak disadari

show abstract

“…Penelitian potensi pengikat aflatoksin (aflatoxin binder) perlu dilakukan secara komprehensif untuk mengetahui jenis dan efektifitasnya (Diaz et al, 2004). Pengikat aflatoksin yang banyak diteliti diantaranya adalah karbon aktif yang dapat digunakan untuk menyerap toksin pada kasus keracunan dan sebagai antidota universal (Widiyanti dan Maryam, 2016 Pakan eksperimental terdiri dari jagung yang terkontaminasi aflatoksin, bekatul, dan konsentrat ayam (Istiqomah et al, 2017). Secara singkat, jagung direndam selama semalam, ditiriskan dan diautoklaf pada suhu 121 o C selama 55 menit.…”

unclassified

“…Biakan A. flavus yang telah ditumbuhkan, diambil konidia dan miseliumnya dengan metode scraping, kemudian dimasukkan dalam aquades hingga konsentrasi 5 x 10 7 konidia/mL -1 . Suspensi A. flavus diinokulasikan pada jagung steril, dan diinkubasi pada suhu 28 0 C selama 7 hari (Abbas et al, 2006), kemudian dikeringkan dibawah sinar matahari dan dihomogenkan (Istiqomah et al, 2017). Jagung yang mengandung aflatoksin dicampur dengan bekatul dan konsentrat ayam untuk mendapatkan nilai nutrisi sesuai dengan Tabel 1.…”

unclassified

Potensi Bentonite Clay Dan Karbon Aktif Sebagai Aflatoksin Binders Berdasarkan Gambaran Imunologis Dan Histopatologis Pada Ayam Buras

Ramandani

Ummami

Hidayah

et al. 2020

J. Il. Pet. Vet. Trop.

View full text Add to dashboard Cite

Aflatoxin is a toxic compound from the fungus A. flavus which can cause a decrease in chicken productivity. This study aims to evaluate the effect of bentonite clay and activated charcoal in chicken feed as aflatoxin binders based on histopathological and immunological features in chickens. A total of 60 chickens were randomly divided into 4 groups (G0, G1, G2, and G3) each group consisted of 15 chickens. G0 as a control without the addition of aflatoxin to the feed. Groups G1, G2, and G3’s feed were added with 106 ppb of aflatoxin. G2 was treated with 5g/kg bentonite clay, G3 was treated with 5g/kg activated charcoal. Chickens were vaccinated with Newcastle Disease (ND) on day 5. On days 14 and 28 serum was collected from the specimens for titer antibody testing. At the end of the study the chickens were dissected, the liver was taken for a histopathological test. The results of the aflatoxin level in the G0, G1, G2, and G3 groups were 10.5, 106, 19.1, and 23.95 ppb, respectively. Microscopic changes show the negative effects of aflatoxin on the liver organ. Group G0 did not show any histopathological changes, Group G2 and G3 showed several mild histopathological changes, whereas group G1 was the most severe. The antibody titers against Newcastle Disease (ND) of 4-weeks old chickens in G0, G1, G2, and G3 were 64, 4, 32, 32 HI units, respectively. The G0 treatment group has a protective titer against ND disease. Group G1 has the lowest (non-protective) ND antibody titer. Whereas the G2 and G3 groups both have protective titers against ND disease. Based on this study it was concluded that the administration of bentonite clay and activated charcoal was able to produce better immunological and histopathological features than the control group.

show abstract

Effect of methionine and lactic acid bacteria as aflatoxin binder on broiler performance

Cited by 7 publications

References 23 publications

Aflatoxin effect on erythrocyte profile and histopathology of broilers given different additives

Aflatoxin effect on erythrocyte profile and histopathology of broilers given different additives

AKTIVITAS ANTIBAKTERI DAUN KIRINYUH (Chromolaena odorata (L.) R.M.King & H.Rob.) TERHADAP BAKTERI GANGREN

Potensi Bentonite Clay Dan Karbon Aktif Sebagai Aflatoksin Binders Berdasarkan Gambaran Imunologis Dan Histopatologis Pada Ayam Buras

Contact Info

Product

Resources

About