Effect of methionine deficiency on oxidative stress and apoptosis in the small intestine of broilers

Ruan, Tao; Lyu, Yingnan; Luo, Qian; Wu, Biao

doi:10.1556/004.2018.006

Cited by 22 publications

(11 citation statements)

References 37 publications

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“…Additionally, contrary to our results, study of Ruan et al showed that methionine deficiency led to oxidative stress in the small intestine of broilers (Ruan et al 2018). The explanation for the protective effect of methionine lies in the fact that methionine residues may have antioxidant properties, protecting the molecules where they are located.…”

Section: Discussioncontrasting

confidence: 99%

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Todorović

Stojanović²,

Šćepanović³

et al. 2019

gpb

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This study was conducted to explore the effects of sulfur containing amino acids on redox status and morphological parameters in the rat ileum tissue. Male Wistar albino rats were randomly divided into the following groups: K group (saline, 1 ml/day, i.p.), M group (methionine, 0.8 mmol/ kg/day, i.p.), C group (methionine (0.8 mmol/kg/day) + L-cysteine (7 mg/kg/day), i.p.) and N group (methionine (0.8 mmol/kg/day) + N-acetyl-L-cysteine (50 mg/kg/day), i.p.). Activities of antioxidant enzymes in the ileum were analyzed to profile oxidative status. Morphometric analysis included measurement of villus height (μm), tunica mucosa thickness (μm), tunica muscularis thickness (μm), the total thickness of the ileal wall (μm) and the number of cells in the lamina propria (per 0.1 mm 2 of tissue). Results showed that methionine treatment reduced the activity of antioxidant enzymes (SOD, GPx, CAT) and the GSH content compared to the control group (p > 0.05). The application of methionine reduced the following parameters statistically significant compared to the control group: length of the ileal villi (p < 0.01), tunica mucosa thickness (p < 0.01), and ileal wall thickness (p < 0.01). We concluded that methionine induced the changes in the gut redox status, which implied oxidative stress occurrence. L-cysteine and N-acetyl-L-cysteine both exhibited antioxidant properties.

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Section: Discussioncontrasting

confidence: 99%

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Todorović

Stojanović²,

Šćepanović³

et al. 2019

gpb

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“…Also, Jankowski, et al (27) reported that increasing methionine level in broiler diets resulted in a significant increase in glutathione concentration and decreasing both malondialdehyde and plasma triglycerides levels. Furthermore, Ruan, et al (28) reported that 120 one day old broilers supplemented with methionine deficient diet resulted in decreasing SOD, catalase and glutathione peroxidase. Similarly, Wang, et al (29) reported that adding L. carnitine at a dose of 100 mg/kg to broilers diet decreased MDA level in heart tissue, triglycerides content and increased SOD and GR.…”

Section: Effect Of the 4 Supplements On Alt And Astmentioning

confidence: 99%

The Role of L. Methionine, L. Carnitine, Choline and/or Silymarin in Hepatoprotection Against Paracetamol Intoxication and Oxidative Stress in Broilers

Aziz¹,

Zahra²,

Kheder³

et al. 2019

SVR

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Paracetamol (Acetaminophen) was used for long time in poultry as an antipyretic drug and as a growth stimulator. However, high doses of paracetamol cause unpleasant side effects such as hepatorenal toxicity as mirrored by depletion of glutathione reserve, increase lipid peroxidation and increase liver enzymes or even sudden death with lethal doses. Therefore, this study was designated to evaluate the ameliorative effect of L. methionine, L. carnitine, choline and/or silymarin on the hepatotoxic effect induced by high doses of paracetamol. The study was applied on 80 chicks (from 1 till 33 days old) in special cages divided into 8 groups each one contained 10 chicks. The 1 st group was used as a control, while the next 2-5 groups were supplemented with L. methionine, L. carnitine, choline and silymarin, respectively with doses as recommended by NRC, the 5 th group was supplemented with silymarin (1 g/kg diet), the 6 th group was supplemented with a mixture of the 4 supplements, the 7 th group (hepatic intoxicated group) was given paracetamol (650 mg/kg diet for 7 days), and the 8 th group was administrated paracetamol with a mixture of the 4 supplements. Serum samples were collected to determine levels of lipid profile [triglycerides (TG) and total cholesterol (TC)], liver damage enzymes [alanine transaminase (ALT), aspartate transferase (AST)], lipid peroxidation marker malondialdehyde (MDA), and activity of antioxidant enzymes [glutathione reductase (GR), superoxide dismutase (SOD)]. The obtained results revealed that there was a significant improvement in all measured serum biochemical parameters and final body weight gain in the combined group (4 supplements + paracetamol) as compared to the paracetamol group. These data conclude that supplementation of poultry diets with L. methionine, L. carnitine, choline and silymarin can improve the negative effect of paracetamol through increasing the body weight gain and antioxidant activity of glutathione and superoxide dismutase and decreasing malondialdehyde, liver enzymes, cholesterol and triglycerides.

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“…Mou et al (2020b) fed organic selenium to pregnant sows and found this significantly increased the activity of GSH-Px in the serum and placenta of the sows, and significantly decreased the content of MDA (Mou et al, 2020b). Other studies showed that the activity of GSH-Px in the duodenum, jejunum, and rectum of chicks fed Se-deficient diets were significantly lower than those in basal diets (Ruan et al, 2018). The present study showed that HMSEBA significantly increased the activities of GSH-Px, T-SOD, and CAT in the thymus, jejunum, ileum, and colon of gilts, and the content of MDA in each tissue showed a decreasing trend.…”

Section: Discussionmentioning

confidence: 92%

Organic Selenium Increased Gilts Antioxidant Capacity, Immune Function, and Changed Intestinal Microbiota

Dong

Chen

et al. 2021

Front. Microbiol.

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Selenium is an indispensable essential micronutrient for humans and animals, and it can affect biological functions by combining into selenoproteins. The purpose of this study was to investigate the effects of 2-hydroxy-4-methylselenobutanoic acid (HMSeBA) on the antioxidant performance, immune function, and intestinal microbiota composition of gilts. From weaning to the 19th day after the second estrus, 36 gilts (Duroc × Landrace × Yorkshire) were assigned to three treatments: control group, sodium selenite group (0.3 mg Se/kg Na2SeO3), and HMSeBA group (0.3 mg Se/kg HMSeBA). Dietary supplementation with HMSeBA improved the gilts tissue selenium content (except in the thymus) and selenoprotein P (SelP1) concentration when compared to the Na2SeO3 or control group. Compared with the control group, the antioxidant enzyme activity in the tissues from gilts in the HMSeBA group was increased, and the concentration of malondialdehyde in the colon had a decreasing trend (p = 0.07). Gilts in the HMSeBA supplemented group had upregulated gene expression of GPX2, GPX4, and SelX in spleen tissue, TrxR1 in thymus; GPX1 and SelX in duodenum, GPX3 and SEPHS2 in jejunum, and GPX1 in the ileum tissues (p < 0.05). In addition, compared with the control group, the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) in the liver, spleen, thymus, duodenum, ileum, and jejunum of gilts in the HMSeBA group were downregulated (p < 0.05), while the expression of interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) in the liver, thymus, jejunum, and ileum were upregulated (p < 0.05). Compared with the control group and the Na2SeO3 group, HMSeBA had increased concentration of serum cytokines interleukin-2 (IL-2) and immunoglobulin G (IgG; p < 0.05), increased concentration of intestinal immunoglobulin A (sIgA; p < 0.05), and decreased concentration of serum IL-6 (p < 0.05). Dietary supplementation with HMSeBA also increased the abundance of intestinal bacteria (Ruminococcaceae and Phascolarctobacterium; p < 0.05) and selectively inhibited the abundance of some bacteria (Parabacteroides and Prevotellaceae; p < 0.05). In short, HMSeBA improves the antioxidant performance and immune function of gilts, and changed the structure of the intestinal microflora. And this study provided data support for the application of HMSeBA in gilt and even pig production.

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Effect of methionine deficiency on oxidative stress and apoptosis in the small intestine of broilers

Cited by 22 publications

References 37 publications

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

The Role of L. Methionine, L. Carnitine, Choline and/or Silymarin in Hepatoprotection Against Paracetamol Intoxication and Oxidative Stress in Broilers

Organic Selenium Increased Gilts Antioxidant Capacity, Immune Function, and Changed Intestinal Microbiota

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