Effect of molecular patch modification on the stability of dynamic high‐pressure microfluidization treated trypsin

Liu, Wei; Zhang, Zhao-Qin; Xie, Mingyong; Liang, Ruihong; Liu, Junping; Zou, Liqiang; Wan, Jie

doi:10.1016/j.ifset.2012.08.001

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…Native amylose could be designated as types A, B, and C according to its X‐ray diffraction patterns. Type A starch was mainly related to maize and wheat starches, giving a stronger diffraction peaks at around 15 o , 17 o , 18 o , and 23 o ; Type B starch was associated with tuber and amylose‐rich starches with stronger peaks at around 17 o and a few small peaks at around 20 o , 22 o , and 24 o ; Type C starch obtained from legume starches displayed a mixture of both A and B patterns (Liu et al, ; Shamai, Bianco‐Peled, & Shimoni, ). In this work, amylose was isolated and purified from rice starch.…”

Section: Resultsmentioning

confidence: 99%

“…DHPM can generate high‐velocity impacts, high‐frequency vibrations, and intense shear, etc., which causes the formation of fine emulsions and modifies the texture of various dairy products. In recent research, DHPM, as an emerging technology, has been employed in the food industry, for example, for modifying proteins (Chen, Tu et al, ), starches (Tu et al, ), enzymes (Liu et al, ), fibers (Wan et al, ), pectin (Zhang, Xie, Lan, Gong, & Wang, ), etc. Chen et al () suggested that DHPM treatment could induce the degradation of pectin owing to the rupture of glyosidic bonds.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and structural characteristics of rice amylose by dynamic high‐pressure microfluidization modification

Wang

Zhu

Zhang

et al. 2018

J Food Process Preserv

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A suspension of rice amylose was subjected to dynamic high‐pressure microfluidization (DHPM) at 60, 100, 140, and 180 MPa and the effect on the microstructure and particle characteristics was investigated. After DHPM treatment, more hollows and grooves were found in the surface of the rice amylose and the particle size decreased and became uniform with an increase in pressure, which increased the specific surface area. DHPM treatment at above 100 MPa was sufficient to cause the complete destruction of the crystalline structure of rice amylose. We proposed a hypothesis of the mechanism, namely, that a decrease in particle size and puffing of granules both occurred after DHPM treatment to yield a uniform and homogeneous system. Moreover, complex DHPM forces could modify the structure and the interaction between amylose and other molecules, playing an important role in some functional properties of amylose. Practical applications Rice is one of the most important staple food in China. Its main component is rice amylose. In this study, the structure and morphology of rice amylose treated by DHPM were analyzed. The results are helpful to understand the mechanism of the modification of amylose induced by DHPM and contribute for us to explain our previous studies of the effect of DHPM on physicochemical and functional properties of rice amylose. Meanwhile, it can also provide theoretical support for starch modification.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological and structural characteristics of rice amylose by dynamic high‐pressure microfluidization modification

Wang

Zhu

Zhang

et al. 2018

J Food Process Preserv

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“…This device uses a high-pressure positive displacement pump (the pressure range is approximately 5-200 MPa). This equipment has been traditionally used in the pharmaceutical industry to make pharmaceutical emulsions as well as in the food industry to produce nanosystems 10,11 or homogenized proteins (milk, whey protein, trypsin, and so on) [12][13][14][15] and dietary fiber 16 only in the last few years. High-pressure microfluidization uses the combined forces of high-velocity impact, high-frequency vibration, instantaneous pressure drop, intense shear, cavitation, and ultra-high pressures up to 200 MPa with a short treatment time (less than 5 seconds) and continuous operation.…”

Section: High-pressure Microfluidizationmentioning

confidence: 99%

A Modern Technology Applied in Traditional Chinese Medicine: Progress and Future of the Nanotechnology in TCM

et al. 2019

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The application of nanotechnology to traditional Chinese medicine (TCM) enabled the development of Chinese medicine in the international society. The pharmacodynamics of TCM is not only depending on its chemical constituents but also related to its physical state such as particle size. Indeed, there is some new pesticide effect that appeared when the medicine was being made into nanophase. The application of nanotechnology to TCM can expand the use of a range of Chinese medicinal materials. In this review, we introduce the concept of nanometer TCM. We also review the preparation methods, advantages, and development tendency of Nano-TCM; furthermore, we analyze the problems in the process of development of Nano-TCM and put forward varies possible solutions to solve this problems, thereby providing new thought for the development of Nano-TCM.

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“…Compared with high-energy wet ball milling, the efficiency of HPH nanoprocessing is extremely high and can reduce the particle size to the nanoscale in a short time (<10 min), while high-energy wet ball milling usually takes 5 h or more [ 3 , 10 , 13 ]. This may be because HPH equipment can simultaneously apply high-speed shear, pressure gradient, high-speed collision and other forces to the raw materials, and these treatment conditions are usually very severe [ 16 , 17 , 18 , 19 ]. Iordache and Jelen [ 20 ] found that HPH could increase the solubility of heat-denatured whey protein, while Chen et al [ 21 ] found that HPH could promote the dissolution of chicken breast myofibrillar proteins in water.…”

Section: Introductionmentioning

confidence: 99%

Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension

et al. 2022

Foods

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As a natural calcium resource, animal bone needs to be miniaturized to the nanoscale to improve palatability and absorption capacity. To explore the mechanism of high-pressure homogenization (HPH) in preparing natural bone aqueous nanosuspensions, the relationships between the changes in protein conformation, solubility and quality characteristics of rabbit bone aqueous suspensions (RBAS) prepared by different HPH cycles were studied. The results showed that the improvements in particle size, stability and calcium solubility of RBASs could be mainly attributed to the improvement of protein solubility induced by the changes in protein conformation. HPH treatment led to the denaturation and degradation of protein in rabbit bone, generating soluble peptides and improving the stability of the suspensions by enhancing the surface charge of the particles. When collagen as the main protein was partially degraded, the hydroxyapatite in the bone was crushed into tiny particles. The increase in the particle-specific surface area led to the release of calcium ions, which chelated with the peptides to produce peptide calcium. However, excessive HPH treatment caused the production of protein macromolecular aggregates and affected the quality of RBASs. This study is helpful to promote the application of HPH technology in animal bone nanoprocessing.

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Effect of molecular patch modification on the stability of dynamic high‐pressure microfluidization treated trypsin

Cited by 8 publications

References 28 publications

Morphological and structural characteristics of rice amylose by dynamic high‐pressure microfluidization modification

Morphological and structural characteristics of rice amylose by dynamic high‐pressure microfluidization modification

A Modern Technology Applied in Traditional Chinese Medicine: Progress and Future of the Nanotechnology in TCM

Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension

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