Effect of Morphine Based CPP on the Hippocampal Astrocytes of Male Wistar Rats

Shaabani, R.; Jahanshahi, Mehrdad; Nowrouzian, M.; Sadeghi, Yousef; Azami, Nasrin-Sadat

doi:10.3923/ajcb.2011.89.96

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…The number of immunoreactive cells was determined to estimate the percentage of immunoreactive cells. A random table was utilized to select the fields, and 200 cells were counted ( Nasiri et al, 2019 ; Shaabani et al, 2011 ).…”

Section: Methodsmentioning

confidence: 99%

“…The number of immunoreactive cells was determined to estimate the percentage of immunoreactive cells. A random table was utilized to select fields, and a total of 200 cells were counted(Nasiri et al, 2019;Shaabani, Jahanshahi, Nowrouzian, Sadeghi, & Azami, 2011).2.8. Determination of dopamine by high-performance liquid chromatography (HPLC)Dopamine levels were measured using HPLC at 20 days in vitro (DIV).…”

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confidence: 99%

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Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

Moayeri

Alizadeh

Hamidabadi

et al. 2022

BCN

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Introduction: The induction of from human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases including Parkinson’s disease. This study aimed to differentiate HUC-MSCs into dopaminergic neuron-like cells. Methods: After HUC-MSCs isolation and characterizations, they transferred onto matrigel- coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2D culture and on matrigel was assessed by real-time PCR, immunocytochemistry and high-performance liquid chromatography (HPLC). Results: Our results showed that the transcript and protein levels of dopaminergic neuronal markers was significantly increased on Matrigel differentiated cells as compared with 2D culture plates. Conclusions: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potentials for the treatment of dopaminergic neuron-related diseases.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

Moayeri

Alizadeh

Hamidabadi

et al. 2022

BCN

View full text Add to dashboard Cite

show abstract

“…Hence, apoptosis, also known as programmed cell death, contributes to neuronal cell death in AD as a result of trophic factor deprivation [6]. The most important key factors involved in apoptosis are the B-cell lymphoma 2 (BCL2) family (mainly anti-apoptotic BCL2 and pro-apoptotic BAX) and the caspase family (mainly caspase 3) [5,7].…”

Section: Introductionmentioning

confidence: 99%

Intranasal Delivery of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Alleviate Memory Deficits In Sporadic Alzheimer’s Rat Model By Regulating Neurotrophic and Apoptosis Genes

Eslami

Qiyami-Hour

Sadr

et al. 2021

Preprint

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Alzheimer's disease (AD) is the most common cause of dementia in adulthood, which is followed by cognitive impairment and behavioral deficits. Today, mesenchymal stem cell (MSC)-based therapy is a good therapeutic option to improve regenerative medicine in neurodegenerative disorders including AD. The aim of this study is to investigate the effects of the human Wharton’s jelly-derived MSCs (WJ-MSCs) on Alzheimer's rat models by studying the expression of neurotrophic factors involved in neurodegenerative diseases such as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), as well as expression of apoptotic factors such as B-cell lymphoma 2 (BCL2, apoptosis inhibitor), BCL2-associated X protein (BAX, apoptosis initiator), and Caspase 3 (apoptosis executioner). Rat AD modeling was performed by intrahippocampal injection of amyloid β 1–42 (Aβ 1–42, 8 µg/kg). Animals were divided into 3 groups of 8 rat: I) control II) AD model III) MSC-treated. Behavioral tests (i.e. Passive Avoidance and Morris Water Maze) showed cognitive improvement, and amelioration of cells in the CA1 area of the hippocampus has been detected by cresyl violet (nissl) staining. Also, real-time polymerase chain reaction (RT-PCR) of the hippocampus indicated an increase in BDNF and NGF genes and a decrease in apoptosis-related genes (BCL2, BAX, and Caspase 3). Overall, WJ-MSCs improved cognitive functions in AD rat models by increasing neurotrophic factors and decreasing apoptotic factors.

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“…The most important factors involved in apoptosis include the B-cell lymphoma 2 (BCL2) family (mainly anti-apoptotic BCL2 and pro-apoptotic BAX) and the caspase family (mainly caspase 3) [5,7]. Currently, there is no de nitive treatment for AD, and available treatments, such as administration of acetylcholinesterase inhibitors (AchEI), can only reduce the symptoms, with possible side effects [8,9], which necessitate an effective treatment.…”

Section: Introductionmentioning

confidence: 99%

Intranasal Delivery of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Alleviates Memory Deficits In Sporadic Alzheimer’s Rat Models By Regulating Neurotrophic and Apoptotic Genes

Eslami

Hour

Sadr

et al. 2021

Preprint

View full text Add to dashboard Cite

Alzheimer's disease (AD) is the most common cause of dementia in adulthood, followed by cognitive and behavioral deficits. Today, mesenchymal stem cell (MSC)-based therapy is a suitable therapeutic option to improve regenerative medicine approaches against neurodegenerative disorders, including AD. This study aimed to investigate the effects of human Wharton’s jelly-derived MSCs (WJ-MSCs) on Alzheimer's rat models by evaluating the expression of neurotrophic factors involved in neurodegenerative diseases, such as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), as well as the expression of apoptotic factors, such as B-cell lymphoma 2 (BCL2, an apoptosis inhibitor), BCL2-associated X protein (BAX, an apoptosis initiator), and caspase 3 (an apoptosis executioner). The AD rat modeling was performed by intrahippocampal injection of 8 µg/kg of amyloid β1-42 (Aβ1-42). The animals were divided into three groups of eight rats each: I) control; II) AD model; and III) MSC-treated model. Behavioral tests (i.e., passive avoidance and Morris water maze tests) showed cognitive improvements. Also, amelioration of cells in the CA1 area of the hippocampus was detected by cresyl violet (Nissl) staining. Besides, real-time polymerase chain reaction (RT-PCR) of the hippocampus indicated an increase in BDNF and NGF genes and a decrease in apoptosis-related genes (BCL2, BAX, and caspase 3). Overall, the WJ-MSCs improved the cognitive function in AD rat models by increasing neurotrophic factors and decreasing apoptotic factors.

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Effect of Morphine Based CPP on the Hippocampal Astrocytes of Male Wistar Rats

Cited by 9 publications

References 13 publications

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

Intranasal Delivery of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Alleviate Memory Deficits In Sporadic Alzheimer’s Rat Model By Regulating Neurotrophic and Apoptosis Genes

Intranasal Delivery of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Alleviates Memory Deficits In Sporadic Alzheimer’s Rat Models By Regulating Neurotrophic and Apoptotic Genes

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