Purpose: Poly(methyl methacrylate) (PMMA) is the most commonly used material in the production of dental prostheses, and its application is often accompanied by the formation of biofilm. The aim of this work was the preparation of a PMMA/gold nanoparticles (AuNps) composite to improve the antimicrobial properties of heat-polymerised PMMA. The AuNPs were synthesised from gold (III) acetate by Ultrasonic Spray Pyrolysis (USP). In the present study, flexural strength and elastic modulus were investigated, as well as thermal conductivity, density and hardness of the PMMA/ AuNps` nanocomposite, with different concentrations of AuNps. Flexural strength and elastic modulus were measured using a three-point bending test, and surface hardness was evaluated using the Vickers hardness test. The thermal conductivity of the samples was measured using the Transient Plane Source (TPS) technique. Density was determined by the pycnometry procedure. Statistical analysis was conducted on the data obtained from the experiments. Results: The flexural strength and elastic modulus of AuNps/PMMA nanocomposites decreased for all groups containing AuNps. Thermal conductivity and density increased in all groups containing AuNps compared to the control group, but it was not significant in all groups. Vickers hardness values increased significantly with an increase in AuNps` content, with the highest value 21.45 HV obtained at 0.74 wt% of AuNps. Statistical analysis was performed by means of the SPSS 19 software package. Conclusions: Incorporation of AuNps into heat-polymerised PMMA resin led to decrease of the flexural strength and elastic modulus. At the same time, the density, thermal conductivity and hardness increased.