Effect of natural antioxidants on frozen bovine semen preservation

Beconi, M.T.; Francia, C.R.; Mora, N; Affranchino, M.A.

doi:10.1016/0093-691x(93)90219-u

Cited by 131 publications

(99 citation statements)

References 16 publications

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“…Increase in lipid peroxidation of spermatozoa during cryopreservation has been attributed to the reduction of antioxidant enzymes after semen extension (Bilodeau et al 2000;Baumber et al 2003), and higher activity of aromatic amino oxidase enzyme from cryo-injured or dead spermatozoa (Upreti et al 1998). As a result, frozen-thawed spermatozoa are easily peroxidised than fresh spermatozoa (Beconi et al 1993). In support, we found that the magnitude of the progressive decline in the semen quality of frozen-thawed spermatozoa was similar between the age groups (Table 2); correspondingly indicating that lipid peroxidation is mainly the outcome of cryopreservation rather than age.…”

Section: Discussionmentioning

confidence: 99%

Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls

Ahmed

Khan

Ahmad

et al. 2018

Italian Journal of Animal Science

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Buffalo bull spermatozoa are rich in polyunsaturated fatty acids and are prone to lipid peroxidation. We hypothesised that lipid peroxidation in buffalo bull semen will increase with age and will affect the semen quality. The objective was to compare malondialdehyde (MDA) concentration and the quality of fresh and frozen-thawed semen between aged (13.6 ± 1.0 years; n ¼ 3) and young (3.4 ± 0.3 years; n ¼ 3) Nili-Ravi bulls. The concentration of MDA did not differ (p >.05) between aged vs. young bulls in fresh (2.3 ± 0.2 vs. 2.9 ± 0.7 nmol mL À1 ), frozen-thawed (53.1 ± 2.8 vs. 48.4 ± 2.6 nmol mL À1 ) semen and seminal plasma (5.71 ± 0.97 vs. 5.19 ± 1.36 nmol mL À1 ), respectively. In fresh semen, sperm motility and total concentration did not differ (p > .05) between aged and young bulls. The volume of fresh semen increased (p > .05) while sperm viability and DNA integrity decreased (p < .05) in aged vs. young bulls. In frozen-thawed semen, sperm motility, viability and DNA integrity decreased (p < .05) in aged vs. young bulls. In frozen-thawed vs. fresh semen, MDA level increased within young (48.4 ± 2.6 vs. 2.3 ± 0.2 nmol mL À1 ) and aged bulls (53.1 ± 2.8 vs. 2.9 ± 0.7 nmol mL À1 ). Conversely, sperm motility and viability decreased (p < .05) within the age groups between fresh and frozen-thawed semen.In conclusion, (1) lipid peroxidation (MDA) does not increase due to age; however, it is negatively associated with semen quality and (2) cryopreservation steps up lipid peroxidation irrespective of age and deteriorates semen quality of Nili-Ravi bulls.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls

Ahmed

Khan

Ahmad

et al. 2018

Italian Journal of Animal Science

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“…These can lead to the formation of ROS, which damage sperm motility and genomic integrity (Foote and Hare, 2000;Bilodeau et al, 2001). Under these -E-mail: zanls@yahoo.com.cn conditions, additional antioxidants can protect against the damaging effect of ROS on sperm movement, and help to maintain sperm motility (Beconi et al, 1993). Additions of various antioxidants to spermatozoa have been investigated in the frozen-thawed semen of bulls (Beconi et al, 1993), humans (Askari et al, 1994) and boars (Pena et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of vitamin E supplementation in the extender on frozen-thawed bovine semen preservation

Zhao²,

Tian

et al. 2011

Animal

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The maturing sperm cells discard the majority of their cytoplasm during the final stages of spermatogenesis and lose some of their defense enzymes. The purpose of this study was to investigate the effects of vitamin E supplementation on standard semen quality parameters and antioxidant activities of frozen-thawed bovine sperm. Vitamin E was added at concentrations of 0.5, 1.0, 1.5 and 2.0 mg/ml to bovine semen cryoprotective medium. The results showed that the sperm motility and VSL, STR values in the extender supplemented with 1.0 and 1.5 mg/ml of vitamin E, were significantly higher than that of other concentrations ( P , 0.05). The percentages of acrosome-intact and membrane-intact sperm were significantly improved ( P , 0.05) by supplementing with 1.5 mg/ml of vitamin E. In biochemical assays, the extender supplemented with vitamin E did not exhibit significant improvement in SOD (superoxide dismutase) levels, compared with the control ( P . 0.05). Compared with other groups, CAT (catalase) levels were demonstrated to be greater with the supplementation of vitamin E at 1.0 and 1.5 mg/ml ( P , 0.05). The extender supplemented with 1.5 mg/ml of vitamin E caused the highest levels of glutathione peroxidase (GSH-Px), compared with other groups ( P , 0.05). The glutathione (GSH) activity was significantly higher with the supplementation of 0.5, 1.0 and 1.5 mg/ml of vitamin E, compared with 2.0 mg/ml in the vitamin E group and control ( P , 0.05). Moreover, increasing the doses of vitamin E decreased sperm antioxidant activities, the extender supplemented with 2.0 mg/ml of vitamin E, caused the lowest levels of GSH-Px and GSH activities, compared with other treatment groups ( P , 0.05). In conclusion, the beneficial effects of vitamin E noted in this study can be attributed to the antioxidant characteristics. Vitamin E supplementation in the extender reduced the lipid peroxidation potential and improved semen quality during freezing-thawing. More researches are needed to evaluate and understand the precise physiological role of vitamin E in reproduction.Keywords: bovine semen, vitamin E, cryopreservation, standard semen quality parameters, antioxidant activities ImplicationsBovine semen cryopreservation allows the widespread dissemination of valuable genetic material. However, the overproduction of reactive oxygen species (ROS) causes sperm membrane structural damage and results in the loss of 40% to 50% of viable sperm during freeze-thaw. As the primary component of the sperm antioxidant system, vitamin E is the major membrane protectant against ROS. No data seem to be available regarding the effects of vitamin E on frozen-thawed semen of the Chinese Holstein Cattle. This study was set up to determine the effects of vitamin E on standard semen parameters and antioxidant activities following the freeze-thawing of bovine semen.

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“…Spermatozoa yang sudah mengalami hiperaktifasi dan tidak terjadi fertilisasi maka spermatozoa tersebut akan segera mengalami kematian (Feradis, 2010;Susilawati, 2011 Semen yang ditambah filtrat kecambah kacang hijau pada pengencer skim milk tidak dapat mempertahankan viabilitas spermatozoa setelah penyimpanan suhu dingin, diduga sudah terjadi peroksidasi lipid sebelum penambahan filtrat kecambah kacang hijau. Beconi, et al (1993) menyatakan bahwa antioksidan yang ditambahkan pada semen dengan kualitas rendah tidak mampu menghentikan proses peroksidasi lipid.…”

Section: Materi Dan Metodeunclassified

PENGARUH SUPLEMENTASI FILTRAT KECAMBAH KACANG HIJAU (Phaseolus radiatus L.) TERHADAP KUALITAS SEMEN SAPI SIMMENTAL DALAM PENGENCER SKIM MILK PADA SUHU DINGIN

Sades

Isnaini

Wahjuningsih

2016

JTAPRO

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ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh suplementasi filtrat kecambah kacang hijau dalam pengencer skim milk terhadap kualitas semen sapi Simmental pada suhu dingin. Materi penelitian yang digunakan adalah semen pejantan sapi Simmental dengan kriteria umur 5-7 tahun dan memiliki motilitas individu ≥50%. Metode penelitian yang digunakan adalah pola Rancangan Acak Kelompok (RAK) dengan 4 perlakuan dan 6 kali ulangan. Semen diencerkan dalam pengencer skim milk dengan 4 perlakuan (0%, 2%, 4% dan 6% filtrat kecambah kacang hijau). Hasil penelitian menunjukkan bahwa penambahan filtrat kecambah kacang hijau ke dalam pengencer skim milk memberikan perbedaan yang tidak nyata (P>0,05) terhadap motilitas, viabilitas dan abnormalitas spermatozoa. Kesimpulan dari penelitian ini adalah penambahan filtrat kecambah kacang hijau dalam pengencer skim milk tidak dapat mempertahankan kualitas semen cair (motilitas, viabilitas dan abnormalitas) sapi Simmental yang disimpan pada suhu dingin. Saran untuk penelitian selanjutnya adalah perlu studi lebih lanjut mengenai pengaruh suplementasi filtrat kecambah kacang hijau dalam pengencer skim milk terhadap kualitas semen cair sapi Simmental dengan menggunakan semen segar yang memiliki motilitas awal sesuai standart yaitu ≥70%.Kata kunci: kualitas spermatozoa, semen cair, penyimpanan suhu dingin. ABSTRACTThe purposes of this research was to evaluate the effect of supplementation mung bean sprouts filtrate on diluent skim milk to quality of Simmental bulls semen in cold temperatures. The materials that used were Simmental bulls semen with 5-7 years old and motility ≥50%. Method was used in this research was Randomized Block Design with 4 treatment and 6 replication. Semen was diluted in skim milk diluent with four treatments (0%, 2%, 4% and 6% filtrate of mung bean sprouts). The result of the research showed that filtrate of mung bean sprouts in diluent skim milk was no significant effect (P>0.05) on motility, viability and abnormalities of spermatozoa. The conclusion of this research was additional of mung bean sprouts filtrate in a diluent skim milk was not able to maintain liquid semen quality (motility, viability and abnormalities) of Simmental bulls in cold temperatures. Suggestions for future research was to determine the effect of supplementation mung bean sprouts filtrate on diluent skim milk in cold temperatures was recommended to used semen with motility ≥70%.

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Effect of natural antioxidants on frozen bovine semen preservation

Cited by 131 publications

References 16 publications

Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls

Effect of age on lipid peroxidation of fresh and frozen-thawed semen of Nili-Ravi buffalo bulls

Effects of vitamin E supplementation in the extender on frozen-thawed bovine semen preservation

PENGARUH SUPLEMENTASI FILTRAT KECAMBAH KACANG HIJAU (Phaseolus radiatus L.) TERHADAP KUALITAS SEMEN SAPI SIMMENTAL DALAM PENGENCER SKIM MILK PADA SUHU DINGIN

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