Effect of natural commensal-origin DNA on toll-like receptor 9 (TLR9) signaling cascade, chemokine IL-8 expression, and barrier integritiy of polarized intestinal epithelial cells

Ghadimi, Darab; Vrese, Michael de; Heller, Knut J.; Schrezenmeir, J.

doi:10.1002/ibd.21057

Cited by 107 publications

(79 citation statements)

References 65 publications

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“…Examples of its proven clinical benefits include preventing and relieving certain types of diarrhea (11), reducing the incidence of respiratory infections in children (15) and impeding atopic disease (18). Up to now, only a few probiotic effector molecules, including lactic acid as an antimicrobial agent against Salmonella enterica serovar Typhimurium (7), secreted proteins that mediate homeostasis of intestinal epithelial cells (IECs) (43), and genomic DNA with anti-inflammatory effects in IECs (9), have been identified in L. rhamnosus GG. We have chosen this strain as a model for genetic studies to identify other probiotic molecules.…”

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confidence: 99%

Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

Lebeer

Claes

Tytgat

et al. 2012

Appl Environ Microbiol

288

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confidence: 99%

Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

Lebeer

Claes

Tytgat

et al. 2012

Appl Environ Microbiol

288

301

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“…El epitelio intestinal está constantemente expuesto a grandes cantidades de antígenos alimenticios y bacterianos. En condiciones fisiológicas normales, la monocapa epitelial intestinal facilita un flujo controlado y selectivo de componentes entre el lúmen y la mucosa (Ghadimi et al, 2010). El balance entre la tolerancia y la inmunidad en el intestino está, en parte, determinada por poblaciones de células presentadoras de antígeno (CPA) en el intestino.…”

Section: Discussionunclassified

La adición de Enterococcus faecium aumenta la respuesta inmune intestinal en cerdos en crecimiento

2016

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Destete. Lechones. Probióticos. Sistema inmune. resUMenEl destete produce un periodo breve de ayuno y adaptación a una nueva ración sólida, la cual provoca disminución de la supervivencia de los lechones y proliferación de la microbiota patógena. Se ha propuesto la utilización de probióticos, evitando que los seres humanos consuman alimentados tratados con antibióticos. El objetivo de este trabajo fue determinar el efecto de la adición de cepas de probióticos en cerdos en crecimiento sobre las poblaciones celulares del sistema inmune (eosinófilos, basófilos, neutrófilos, monocitos y linfocitos) en intestino delgado. Se sacrificaron 35 lechones escalonadamente los días 1 (21 días de edad), 15 y 30 posdetete, y se extrajo completamente el intestino. Los animales fueron alimentados con dos dietas: dieta comercial con y sin la adición de antibiótico; a esta última se adicionaron los diferentes probióticos (Lactobacillus casei, Lactobacillus acidophilus o Enterococcus faecium) en el agua de bebida. Se utilizó un diseño de bloques al azar en un arreglo de parcelas divididas. Los aislamientos realizados a partir de las muestras intestinales de E. faecium presentaron un mayor número de poblaciones celulares del sistema inmune a nivel intestinal (p<0.01). Además, se realizó medición del pH intestinal, el cual presentó una disminución estadística significativa (p<0.01), donde los animales que consumieron E. faecium presentaron los valores más bajos de pH intestinal, frente a aquellos que consumieron la dieta con adición de antibiótico. La adición de la cepa E. faecium en el alimento de lechones recién destetados, estimula el aumento en el número de poblaciones celulares intestinales del sistema inmune y alcanzan una mayor disminución del pH intestinal. Weaning originates a brief period of fasting and adaptation to a new solid portion, which causes decreased survival of piglets and proliferation of pathogenic microbiota. It has proposed the use of probiotics, preventing humans consume fed treated with antibiotics. Therefore, the objective of this work was to determine the effect of the addition of probiotic strains in growing pigs on immune cell populations (eosinophils, basophils, neutrophils, monocytes and lymphocytes) in the small intestine. Thirty-five piglets stepwise days 1 (21 days old), 15 and 30 posdetete were sacrificed, and the intestine was completely removed. The animals were fed two diets: commercial diet with and without the addition of antibiotic; the latter different probiotic (Lactobacillus casei, Lactobacillus acidophilus or Enterococcus faecium) were added in the drinking water. A design randomized block was used in a split plot arrangement. Isolates obtained from the intestinal samples of E. faecium showed a greater number of immunological intestine cell populations (p<0,01). In addition, measurement of intestinal pH, which showed a statistically significant decrease (p<0,01), where the animals fed E. faecium reported lower intestinal pH values, compared to those who ate the diet with addition of antib...

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“…These studies included one human intervention study (de Vrese et al, 2005), one animal study (Ohno et al, 2005) and three in vitro studies (Ghadimi et al, 2008;2010a;2010b).…”

Section: Scientific Substantiation Of the Claimed Effect (Id 931)mentioning

confidence: 99%

“…One animal study (Ohno et al, 2005) and three in vitro studies (Ghadimi et al, 2008;2010a;2010b) explored the effects of the microorganisms on the immune response to allergens/antigens. Three of these studies examined the effects of a single strain and only the in vitro …”

Section: Scientific Substantiation Of the Claimed Effect (Id 931)mentioning

confidence: 99%

Scientific Opinion on the substantiation of health claims related to a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 and maintenance of upper respiratory tract defence against pathogens (ID 931, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

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2012

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The claimed effect, which is proposed for further assessment, is "supports the defence against pathogens in the upper respiratory tract resulting in a decrease in duration and severity of common cold symptoms". The proposed target population is the general adult population. The Panel considers that maintenance of upper respiratory defence against pathogens is a beneficial physiological effect.In the only human intervention study provided, the effect of consumption of the bacterial preparation on the incidence, severity and duration of common colds was assessed in healthy adults. The Panel notes that no information is provided about the validity of the questionnaire used to assess the incidence, severity or duration of respiratory tract infections, that the symptoms used for the diagnosis of common cold episodes are non-specific, and that the presence of one or more of these symptoms is not an appropriate measure of respiratory tract infections in the study population. The Panel also notes that no information has been provided in the publication about the use of rescue medication, which may have confounded the results. The Panel considers that no conclusions can be drawn from this study for the scientific substantiation of the claim.The Panel notes that no human intervention studies have been provided from which conclusions could be drawn for the scientific substantiation of the claim, and that the animal and in vitro studies which were provided cannot be used for the scientific substantiation of the claim as their results cannot predict the occurrence of an effect of a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 in vivo in humans.On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the consumption of a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 and maintenance of upper respiratory tract defence against pathogens.

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Effect of natural commensal-origin DNA on toll-like receptor 9 (TLR9) signaling cascade, chemokine IL-8 expression, and barrier integritiy of polarized intestinal epithelial cells

Cited by 107 publications

References 65 publications

Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

La adición de Enterococcus faecium aumenta la respuesta inmune intestinal en cerdos en crecimiento

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