Effect of Nifedipine on the Differentiation of Human Dental Pulp Cells Cultured with Mineral Trioxide Aggregate

Woo, Su-Mi; Hwang, Yun-Chan; Lim, Hee-Chang; Choi, Nam-Ki; Kim, Sun-Hun; Kim, Wonjae; Kim, Seon-Mi; Jung, Ji‐Yeon

doi:10.1016/j.joen.2012.12.037

Cited by 38 publications

(39 citation statements)

References 21 publications

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“…In immunofluorescence analyses, we observed that the signals in MTA- and α-TCP-treated cells were stronger compared to the cells of the control group (Figure 4). It is suggested that the ions released from MTA or CP cement, such as Ca, P, and Si, promote differentiation and mineralization of the cells through ion-mediated reactions [40,42,43]. EDS analysis of the current study revealed that MTA and α-TCP contain these elements, which might affect the expression of the mineralization phenotypes in vitro (Figure 2C and D).…”

Section: Discussionmentioning

confidence: 60%

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Physical properties and biological/odontogenic effects of an experimentally developed fast-setting α-tricalcium phosphate-based pulp capping material

et al. 2014

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Section: Discussionmentioning

confidence: 60%

“…MTA is considered to facilitate odontoblastic differentiation of hDPCs [37-40]. In addition, several studies have shown that CP cement has a similar mineralization ability compared to MTA [35,36,41].…”

Section: Discussionmentioning

confidence: 99%

Physical properties and biological/odontogenic effects of an experimentally developed fast-setting α-tricalcium phosphate-based pulp capping material

et al. 2014

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“…12,13 With this References in mind, the chemical changes made in the cement composition were designed to yield dental materials with good handling characteristic and adequate working time, associated with low cytotoxicity. 21 The present study demonstrated that CER and the experimental MTA formulation not only presented better physical and chemical properties than the conventional white MTA, 12,13 but also exhibited greater toxicity when applied to fibroblast-like MDPL-20 cells than the reference material, at the initial period. The cytotoxicity of the tested cements at the final period of analysis was similar.…”

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confidence: 54%

Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells

et al. 2016

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The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice , in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analysis. Thirty-two round-shaped samples were fabricated with the following cements: white MTA, white and gray CER and experimental white MTA. The samples were immersed in serum-free culture medium for 24 hours or 7 days (n = 16). The extracts (culture medium + components released from the cements) were applied for 24 hours to previously cultured cells (40.000 cells/cm 2 ) in the wells of 24-well plates. Cells seeded in complete culture medium were used as a negative control. Cell viability was assessed using the MTT assay. Two samples of each cement were used for cell morphology analysis by Scanning Electron Microscopy (SEM). The extracts obtained at the 7-day period presented higher cytotoxicity compared with the 24-hour period (p < 0.05). The gray CER obtained at 24 hours presented the highest cytotoxic effect, whereas the experimental white MTA presented the lowest, similar to the control (p > 0.05). However, at the 7-day period, the experimental white MTA presented no significant difference in comparison with the other cements (p > 0.05). At the 7-day period, CER cement presented cytotoxic effects on fibroblast-like cells, similar to different MTA formulations. However, the immersion period in the culture medium influenced the cytotoxicity of the cements, which was greater for CER cement at 24 hours.

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“…Recently, it was also demonstrated that Endocem facilitates the odontogenic differentiation of human dental pulp cells 22) as well as MG63 cells 20) . It was also demonstrated that calcium ions released from MTA-derived cements may affect the odontoblastic/osteoblastic differentiation of human dental pulp cells 44) . EDS analysis performed in this study revealed that EndocemZr contains an amount of Ca similar to ProRoot and Endocem (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…6). There have been several studies showing that MTA-like materials promote the odontogenic/osteogenic differentiation of dental pulp cells [42][43][44][45] . Recently, it was also demonstrated that Endocem facilitates the odontogenic differentiation of human dental pulp cells 22) as well as MG63 cells 20) .…”

Section: Discussionmentioning

confidence: 99%

Odontogenic effects of a fast-setting calcium-silicate cement containing zirconium oxide

et al. 2015

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A fast-setting calcium-silicate cement (Endocem) was introduced in the field of dentistry for use in vital pulp therapy. Similar to mineral trioxide aggregate (MTA), it contains bismuth oxide to provide radiopacity. Recently, another product, EndocemZr, which contains zirconium oxide (ZrO2) as a radiopacifier, was developed by the same company. In this study, the biological/odontogenic effects of EndocemZr were investigated in human primary dental pulp cells (hpDPCs) in vitro and on capped rat teeth in vivo. The biocompatibility of EndocemZr was similar to that of ProRoot and Endocem on the basis of cell viability tests and cell morphological analysis. The mineralization nodule formation, expression of odontogenic-related markers, and reparative dentin formation of EndocemZr group was similar to those of other material groups. Our results suggest that EndocemZr has the potential to be used as an effective material for vital pulp therapy, similar to ProRoot and Endocem.

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Effect of Nifedipine on the Differentiation of Human Dental Pulp Cells Cultured with Mineral Trioxide Aggregate

Cited by 38 publications

References 21 publications

Physical properties and biological/odontogenic effects of an experimentally developed fast-setting α-tricalcium phosphate-based pulp capping material

Physical properties and biological/odontogenic effects of an experimentally developed fast-setting α-tricalcium phosphate-based pulp capping material

Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells

Odontogenic effects of a fast-setting calcium-silicate cement containing zirconium oxide

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