Effect of NPK Media Concentrations on In Vitro Potato Tuberization of Cultivars Nicola and Russet Burbank

Abstract

Our objective was to test whether the NPK levels in commonly used Murashige and Skoog (MS) media are optimal for microtuberiztion for the two cultivars tested. Two N (N 1 =841 and N 2 =1418 mg L −1) ; three P (P 1 =39, P 2 =76.9 and P 3 =115 mg L −1; and two K (K 1 =784 and K 2 =1518 mg L −1 ) media concentrations were evaluated for microtuberization. Higher levels of N 2 P 3 K 2 were more effective for microtuberization than the lower levels of N 1 P 1 K 1 in standard MS media. N 2 P 3 K 2 was the best concentration for all yield components studied, tuber number, tuber weight, and stem and root weight. For the two cultivars, our results suggest that higher NPK levels than t h o s e i n M S m e d i a w o u l d e n h a n c e i n v i t r o microtuberization, and that optimum NPK levels could vary for cultivars.Resumen Nuestro objetivo fue probar si los niveles de NPK en el medio comúnmente usado de Murashige y Skoog son óptimos para la microtuberización en las dos variedades probadas. Se evaluaron dos concentraciones de N en el medio (N1=841 y N2=1418 mg L −1 ); tres de P (P1=39, P2=76.9 y P3=115 mg L −1 ); y dos de K (K1=784 y K2=1518 mg L −1 ) para la microtuberización. Los niveles más altos de N2P3K3 fueron más efectivos que los más bajos N1P1K1 para tal fin en el medio estándar MS (Physiologia Plantarum 15:473-497, 1962). N2P3K2 fue la mejor concentración para todos los componentes de rendimiento estudiados, número y peso de tubérculo, y peso de tallo y raíz. Para las dos variedades, nuestros resultados sugieren que niveles más altos de NPK que los del medio MS estimularían la microtuberización in vitro, y que los niveles óptimos de NPK podrían variar para los cultivares. De las combinaciones de medios NPK probadas, N2P3K2 fue la superior de todas las evaluadas.

Field Performance of Cultivars Nicola and Russet Burbank Micro and Minitubers

“…The use of more appropriate tuberization media (Radouani and Lauer 2015) could substantially increase both microtuber number and weight. By increasing N to 2×, P to 3×, and K to 2× that of the standard MS (Murashige and Skoog 1962) media concentration (control), TTN for Nicola and Russet Burbank was 51 and 76 % higher, respectively, than the control, and TTW was 63 and 39 % higher, respectively, than the control.…”

“…The scheme of microtuber production is described in Radouani and Lauer (2015). Plantlets were cultured for growth in a liquid nutrient medium for 5 weeks.…”

A Model Nutrition Control System in Potato Tissue Culture and Its Influence on Plant Elemental Composition

“…This was the first in vitro study to report the effect of varied P alone and the evaluation of P content in potato plants. Most in vitro studies that have assessed the effect of varied P supply on potato plants were performed in combination with changes in other nutrients [ 7 , 25 ]. In this study, P deficiency symptoms such as weaker stems and darker leaves, as well as fewer and smaller roots, were observed in 0.2 mM N supply ( Figure 2 B).…”

“…Several studies have attempted to adjust MS medium nutrient concentrations for the particular purpose of increasing either plantlet growth or microtuber yield. Radouani and Lauer [ 7 ] simultaneously increased N, P, and K contents from the standard 60 mM, 1.25 mM, and 20 mM, respectively, in MS medium, which resulted in an elevated number of microtubers, microtuber weight, and stem and root weights. The purpose of this study was to increase microtuber yield, and the study did not measure the nutrient contents in the plants.…”

Influence of storey of cuttings of test glass plants and nutrient medium upon induction of formation of tubers of potato in vitro of grades of different groups of ripeness

“…Studies [14] show that even the plants parts which are spliting during the cutting are distinguished by the influence on the induction of microtuber formation and on the resistance of the plants against the viral infection [15]. It should be noted that various cultivars have their reaction to the same conditions of cultivation in in vitro conditions [16][17][18][19][20]. In addition, the complex influence of the differend tiers of steems, potato cultivars of different ripening groups and the composition of the nutrient medium is not well-studied, which means that their complex research acquires an urgency in solving the problem of optimizing the process of tuber formation in in vitro culture.…”