Effect of pH on the association behavior in aqueous solutions of pig gastric mucin

Maleki, Atoosa; Lafitte, Géraldine; Kjøniksen, Anna–Lena; Thuresson, Krister; Nyström, Bo

doi:10.1016/j.carres.2007.10.005

Cited by 55 publications

(43 citation statements)

References 42 publications

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“…3a) as previously described for both commercial and non-commercial preparations. 44,45 Mucin displays a negative -potential at neutral pH which declines as the environment becomes more acidic, and the size of the molecule increases until it reaches a peak at the neutrality point (pH ~2) as shown by the longer relaxation time in Figure 3b. At lower pH values there is a decrease in size, attributed to the contraction of the interchain complexes stabilized by protonated residues.…”

Section: 42mentioning

confidence: 99%

Biophysical Analysis of the Molecular Interactions between Polysaccharides and Mucin

et al. 2015

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Mucoadhesive materials adhere persistently to mucosal surfaces. A mucoadhesive delivery system could therefore facilitate the controlled release of drugs and optimize their bioavailability in mucosal tissues. Polysaccharides are the most versatile class of natural polymers for transmucosal drug delivery. We used microviscosimetry to explore the mucoadhesion of a library of polysaccharide families with diverse structural characteristics as a first step towards the rational design of mucoadhesive polysaccharide-based nanoformulations. Here we show that the magnitude of deviation between the viscosity of mixed polysaccharide-mucin solutions and the corresponding individual stock solutions can indicate underlying molecular interactions. We found that nonlinear monotonic curves predicted a correlation between the magnitude of interaction and the ability of polysaccharide coils to contract in the presence of salt (i.e. chain flexibility). Charge-neutral polysaccharides such as dextran and Streptococcus thermophilus exopolysaccharide did not interact with mucin. Synchrotron small-angle X-ray scattering (SAXS) data supported the previously described structural features of mucin. Furthermore, high-q scattering data (i.e. sensitive to smaller scales) revealed that when mucin is in dilute solution (presumably in an extended conformation) in the presence of low-Mw alginate, its structure resembles that observed at higher concentrations in the absence of alginate. This effect was less pronounced in the case of high-Mw alginate but the latter influenced the bulk properties of mucin-alginate mixtures (e.g. hydrodynamic radius and relative viscosity) more prominently than its low-Mw counterpart.

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Section: 42mentioning

confidence: 99%

Biophysical Analysis of the Molecular Interactions between Polysaccharides and Mucin

et al. 2015

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“…For a review of the structure and properties of mucin and mucus see Bansil et al 2013 [1] and Bansil and Turner 2006 [2]. Understanding the rheology of mucin is especially relevant to its protective and lubricating function, and many rheology studies have been reported on both commercially available gastric mucin [3] and purified porcine gastric mucin, PGM [4,5] which is analogous to human mucin, MUC5AC. Previous work from our group and others has established that purified gastric mucin and mucus forms a viscoelastic gel under acidic conditions at pH below 4 [4,5,6,7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Understanding the rheology of mucin is especially relevant to its protective and lubricating function, and many rheology studies have been reported on both commercially available gastric mucin [3] and purified porcine gastric mucin, PGM [4,5] which is analogous to human mucin, MUC5AC. Previous work from our group and others has established that purified gastric mucin and mucus forms a viscoelastic gel under acidic conditions at pH below 4 [4,5,6,7,8]. Using oscillatory shear rheology Celli et al [5] showed that PGM undergoes a sol-gel transition at pH 4 forming a viscoelastic gel below pH 4 with frequency dependent elastic and viscous moduli, G' and G'' respectively.…”

Section: Introductionmentioning

confidence: 99%

Microrheology of Mucin: Tracking Particles and Helicobacter Pylori Bacteria

Bansil¹,

Hardcastle²,

Constantino³

2015

Epitoanyag - JSBCM

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the gastric ulcer and cancer causing bacteria, helicobacter Pylori have uniquely adapted to swim across the viscoelastic mucus gel that lines the stomach epithelial surface and colonize in the harsh acidic environment of the stomach. in this paper we first briefly review results of bacteria tracking and oscillatory shear rheology studies to suggest how the bacteria get across the viscoelastic mucus gel by using a chemical mechanism to raise the pH from acidic to neutral which also triggers a gel to sol transition of mucin. We then present new microrheology studies to show that the bacterium influences the Brownian motion of spherical tracer particles in culture broth solution and in solutions of gastric mucin. the elastic and viscous moduli obtained by tracking particles in the mucin solutions are found to decrease in the presence of bacteria. We also examined the Brownian motion of the bacteria themselves and find that motile bacteria display super-diffusive anomalous Brownian motion while the immotile bacteria exhibit regular diffusive Brownian motion.

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“…The most dominant component of mucus is mucin [19], which is negatively charged at pH 7.4 [20, 21]. Hence, the repulsion between the negatively charged surfaces and the negatively charged mucus were electrostatic in nature.…”

Section: Resultsmentioning

confidence: 99%

Discovery of low mucus adhesion surfaces

Gu¹,

Yıldız²,

Carrier³

et al. 2013

Acta Biomaterialia

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Mucus secretion from the body is ubiquitous and finding materials that resist mucus adhesion is a major technological challenge of medical and consumer import. Here, using a high throughput platform (HTP) with photo-induced graft polymerization, we first rapidly synthesized, screened and tested a library of 55 different surfaces from six functional monomer classes to discover porcine intestinal low mucus adhesion surfaces using a 1 hr static mucus adsorption protocol. From this preliminary screen, two chemistries, a zwitterionic ([2-(acryloyloxy)ethyl] trimethylammonium chloride) and a multiple hydroxyl (N-[tris(hydroxymethyl)methyl]acrylamide) surface, exhibited the significantly low mucus adhesion from a Langmuir-type isotherm when exposed to increasing concentrations of mucus for 24 hr. Apolar or hydrophobic interactions were likely the dominant attractive forces during mucus binding since many polar or hydrophilic monomers reduced mucus adhesion. Hansen solubility parameters were used to illustrate the importance of monomer polarity and hydrogen-bonding in reducing mucus adsorption. For a series of PEG monomers with changing molecular weight from 144 g/mol to 1100 g/mol, we observed an excellent linear correlation (R2 = 0.998) between relative amount adsorbed and the distance from a water point in a specialized HSP plot, emphasizing the role of surface-water interactions for PEG modified surfaces.

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Effect of pH on the association behavior in aqueous solutions of pig gastric mucin

Cited by 55 publications

References 42 publications

Biophysical Analysis of the Molecular Interactions between Polysaccharides and Mucin

Biophysical Analysis of the Molecular Interactions between Polysaccharides and Mucin

Microrheology of Mucin: Tracking Particles and Helicobacter Pylori Bacteria

Discovery of low mucus adhesion surfaces

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