Effect of photoactivated riboflavin on the biodegradation-resistance of root-dentin collagen

Priyadarshini, Balasankar Meera; Lu, Thong Beng; Fawzy, A.

doi:10.1016/j.jphotobiol.2017.10.012

Cited by 8 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Priyadarshini et al demonstrated that the hydroxyproline content in the supernatant was significantly decreased in riboflavin crosslinked samples than in noncrosslinked samples. 37 Here, the samples were also incubated in collagenase solution for 48 h. 37 In addition, Laggner et al showed that after 120 min of collagenase digestion, an average of 50.5% and 80% of the crosslinked stroma in the 10-and 20-min riboflavin-collagen crosslinked groups remained undigested. 38 In contrast, the corneal stroma in the control group and the 5 min riboflavin-collagen group was completely digested after approximately 100 min.…”

Section: Discussionmentioning

confidence: 99%

Transglutaminase enables highly hydrolytically and proteolytically stable crosslinking of collagen on titanium surfaces and promotes osteogenic differentiation of human mesenchymal stem cells

Palkowitz,

Rüger,

Ziegler

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

Collagen with its bioactive ligand motives would be predestined as coating on bone implant surfaces like titanium hip stems to facilitate receptor‐mediated cell adhesion and thereby improve early osseointegration. Unfortunately, collagen as coating exhibits very low proteolytic resistance in vivo. To overcome this limitation, different crosslinking methods of collagen (transglutaminase, GTA, EDC/NHS, riboflavin, and lysyl oxidase) with silanized titanium alloy (Ti6Al4V) were investigated in terms of degradation resistance, hydrolysis stability, tensile strength, and metabolic cell activity. The in vitro osteogenic differentiation ability of human mesenchymal stem cells (hMSCs) induced by the surface modification was evaluated by immunofluorescence of early osteogenic markers, Alizarin red staining, and energy dispersive X‐ray spectroscopy. The expression of the adhesion‐related protein vinculin was analyzed on the different functionalized surfaces. The results revealed that the enzymatic crosslinker transglutaminase offered high degradation resistance, tensile strength, and hydrolysis stability compared to the other crosslinking reagents tested. Remarkably, the adhesion sequences within the collagen were accessible to the hMSCs despite the transglutaminase crosslinking procedure. In conclusion, the organochemical functionalization of Ti6Al4V surfaces with collagen using transglutaminase holds great potential to facilitate an enhanced interaction with attached bone cells and thereby could potentially improve and accelerate osseointegration of a titanium‐based bone implant in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Transglutaminase enables highly hydrolytically and proteolytically stable crosslinking of collagen on titanium surfaces and promotes osteogenic differentiation of human mesenchymal stem cells

Palkowitz,

Rüger,

Ziegler

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Bovine coccygeal discs have been proposed as a prime physical, biomechanical and biological model for the study of human lumbar discs [19,20]. Bovine tail discs have a large size (area around 430 mm 2 , volume around 3376 mm 3 ) and similar aspect ratio, diffusion distance and resting pressure (0.2-0.3 MPa) to human lumbar discs [19]. Bovine caudal discs have also been found to be compositionally similar to human lumbar discs, with comparable hydration, collagen and proteoglycan profiles and similar rate of proteoglycan synthesis [21].…”

Section: Methodsmentioning

confidence: 99%

“…However, age-dependent degenerative changes lead to a loss of cross-links, and consequently, of biomechanical stability. Therefore, efforts have been made in ophthalmology, dentistry and orthopedics to artificially enhance the biomechanical properties of collagen by efficiently inducing exogenous cross-linking [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Infrared attenuated total reflection spectroscopic surface analysis of bovine‐tail intervertebral discs after UV‐light‐activated riboflavin‐induced collagen cross‐linking

Vasilikos

Haas

Teixeira³

et al. 2020

Journal of Biophotonics

View full text Add to dashboard Cite

The tensile strength of the intervertebral disc (IVD) is mainly maintained by collagen crosslinks. Loss of collagen crosslinking combined with other agerelated degenerative processes contributes to tissue weakening, biomechanical failure, disc herniation and pain. Exogenous collagen cross-linking has been identified as an effective therapeutic approach for restoring IVD tensile strength. The current state-of-the-art method to assess the extent of collagen cross-linking in tissues requires destructive procedures and high-performance liquid chromatography. In this study, we investigated the utility of infrared attenuated total reflection (IR-ATR) spectroscopy as a nondestructive analytical strategy to rapidly evaluate the extent of UV-light-activated riboflavin (B2)induced collagen cross-linking in bovine IVD samples. Thirty-five fresh bovine-tail IVD samples were equally divided into five treatment groups: (a) untreated, (b) cell culture medium Dulbecco's Modified Eagle's Medium only, (c) B2 only, (d) UV-light only and (e) UV-light-B2. A total of 674 measurements have been acquired, and were analyzed via partial least squares discriminant analysis. This classification scheme unambiguously identified individual classes with a sensitivity >91% and specificity >92%. The obtained results demonstrate that IR-ATR spectroscopy reliably differentiates between different

show abstract

“…O uso de 0,1% riboflavina 5'-fosfatado, como agente de cross-linking, pode inativar 55% das MMPs totais, enquanto que, no grupo sem tratamento, a atividade das MMPs aumentou em 84%. A atividade de uma solução de 1% de riblovavina/UV-A por 1 min em dentina radicular desmineralizada com EDTA 17% provocou um aumento na resistência à degradação colagenolítica (37).…”

Section: Riboflavinaunclassified

Efeito dos agentes de Cross-Linking na durabilidade da interface adesiva

Barboza¹,

Maroun

2019

Rev. Naval de Odontologia

View full text Add to dashboard Cite

As metaloproteinases (MMPs) são enzimas colagenolítcas endógenas, capazes de degradar as fibrilas de colágeno presentes na dentina, gerando falhas da interface adesiva. Foram propostos agentes de cross-linking para diminuir essa degradação. O objetivo desta revisão de literatura foi analisar a ação de diferentes agentes de cross-linking sobre as MMPs. A seleção dos artigos foi realizada por meio de uma busca na base de dados PubMed/MEDLINE. A amostra final foi composta por 40 estudos publicados entre 2018 e 2010. Os estudos atuais apresentaram os agentes de cross-linking (cabordiimida, glutaraldeído, proantocianidina, riboflavina/ UV-A e quitosana) com vantagens como inespecificidade em relação aos tipos de MMPs, aumento da resistência da fibra colágena e possibilidade de bloquear o sítio de clivagem da enzima. Observou-se que a cabordiimida, riboflavina/UV-A, o glutaraldeído, a proantocianidina e a quitosana apresentaram resultados positivos na diminuição da degradação da interface adesiva. A carbodiimida e riboflavina/UV-A não são citotóxicas, diferentemente do glutaraldeído. A proantocianidina, quando incorporada no adesivo, apesar de interferir na polimerização dos monômeros adesivos, pode ser efetiva quando utilizada incorporada ao condicionamento ácido. A quitosana é capaz de reforçar as fibrilas de colágeno. Assim, foi possível conhecer mais sobre a ação dos agentes de cross-linking disponíveis. No entanto, há necessidade de mais pesquisas sobre esses agentes.

show abstract

Effect of photoactivated riboflavin on the biodegradation-resistance of root-dentin collagen

Cited by 8 publications

References 30 publications

Transglutaminase enables highly hydrolytically and proteolytically stable crosslinking of collagen on titanium surfaces and promotes osteogenic differentiation of human mesenchymal stem cells

Transglutaminase enables highly hydrolytically and proteolytically stable crosslinking of collagen on titanium surfaces and promotes osteogenic differentiation of human mesenchymal stem cells

Infrared attenuated total reflection spectroscopic surface analysis of bovine‐tail intervertebral discs after UV‐light‐activated riboflavin‐induced collagen cross‐linking

Efeito dos agentes de Cross-Linking na durabilidade da interface adesiva

Contact Info

Product

Resources

About