Effect of pinolenic acid on oxidative stress injury in HepG2 cells induced by H₂O₂

Abstract

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Bioactive polyphenols separated from hemp seed shells ameliorate H2O2‐induced oxidative stress injury in human umbilical vein endothelial cells

“…A series of antioxidant enzymes make up an endogenous antioxidant defense system by converting peroxides into less toxic substances. Intracellular SOD is an important component of the antioxidant defense system, which assists in maintaining the redox balance of organisms by catalyzing the decomposition of superoxide anion and scavenging excess intracellular free radicals (Zhao et al., 2021). As shown in Figure 4g, the SOD activity in the model group reduced significantly by 30.34 ± 2.96% compared with the control group ( p < 0.01).…”

Chemical Composition of Tobacco Seed Oils and Their Antioxidant, Anti-Inflammatory, and Whitening Activities

“…An H 2 O 2- induced oxidative stress model in vitro is generally used to explore the antioxidative effect [ 18 ]. With the stimulation of H 2 O 2 , the cells produce and secrete excessive ROS to destroy the cell antioxidant defense systems [ 19 ]. Therefore, an H 2 O 2 -induced oxidative stress model of HepG2 cells was used in this study to further evaluate the inhibitory effects of these two tobacco seed oils on ROS production.…”

The Beneficial Effects of Pine Nuts and Its Major Fatty Acid, Pinolenic Acid, on Inflammation and Metabolic Perturbations in Inflammatory Disorders

“…Additionally, in comparison to the controls, the pine high-fat diet (PHFD) group had the greatest level of SOD2 mRNA compared to PNO and soybean oil (SBO)-fed groups and soybean high-fat diet (SHFD) groups [ 34 ], which might shield from damage due to ROS. In H 2 O 2 -induced HepG2 cells, 1, 5, and 10 μM of PNLA, dose-dependently reduced intracellular ROS accumulation by 29.1, 59.1, and 65.5% and intracellular MDA content by 15.96%, 20.5%, and 22.9%, respectively [ 35 ]. These data suggest that PNLA reduces ROS-induced lipid peroxidation, thereby decreasing oxidative damage [ 3 ].…”

“…Nuclear factor erythroid 2-related factor 2 (NRF2) was translocated to the nucleus by PNLA in order to protect cells from oxidative stress, and the expression of the antioxidant enzymes heme oxygenase-1 (HO-1) and quinone dehydrogenase-1 that are downstream of NRF2 was also increased [ 35 ]. The NRF2 transcription factor is a key regulator in cellular antioxidant defense responses [ 36 ].…”

“…The NRF2 transcription factor is a key regulator in cellular antioxidant defense responses [ 36 ]. The Kelch-like ECH-associated protein 1 (KEAP1) expression was decreased; according to Zhao et al, these changes also activate the production of antioxidant enzymes leading to a decrease in MDA production, which protects against damage by ROS [ 35 ].…”