Effect of Plant Growth Regulators on Callus Induction and Plantlet Regeneration of Bitter Apple (Citrullus colocynthis) from Stem Explant

Satyavani, K.; Ramanathan, T.; Selvaraj, Gurudeeban

doi:10.3923/ajbkr.2011.246.253

Cited by 31 publications

(13 citation statements)

References 2 publications

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“…Some related research indicated that 2, 4-D played a more important role in callus formation from leaf and stem explants compared to BA (Satyavani, Ramanathan, & Gurudeeban, 2011).But in this experiment, Calli were successfully generated in all treatments regardless of 2, 4-D concentration and different types of explants (Table 1).These results showed that leaf and stem explants were suitable for callus induction. Similar results were reported in callus induction of Corydalis saxicola and Orthosiphon stamineus, in which it were described that callus could be induced successfully from leaf, petiole, stem (Cheng, Yu, Hu, Chen, & Sun, 2006).…”

Section: Effect Of 2 4-d and The Types Of Explants On Callus Inductionmentioning

confidence: 58%

Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema Indicum var. Aromaticum a Scented Chrysanthemum

Jin¹,

Yang²,

Gao³

et al. 2017

JPS

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Dendranthema indicum var. aromaticum is an important aroma plant in genus Dendrathema, and the establishment of callus cultures and cell suspension cultures is the basement of further protoplast fusion studies, which make it possible to breed new fragrant chrysanthemum. In this study, the effects of different plant growth regulating substances in different concentrations on callus induction were investigated with stem segments, leaves, petioles as explants. The results showed that the optimal explants were lower stem segments according to the percentage of callus formation, callus hardness, growth potential and shoot differentiation. The optimal induction mediums were MS supplemented with 1.0 mg ． l -1 2.4 D and 0.2 mg ． l -1 6-BA. The cell suspension culture system was established by using the subculture calli. The results showed that the suitable inoculum size was 2g and the suitable cell suspension culture medium was MS supplemented with 0.2 mg ． l -1 6-BA and 0.5 mg ． l -1 2,4-D. The time course of cell growth showed that the greatest cell fresh weight appeared on day 14 and the highest cell viability on day 3.

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Section: Effect Of 2 4-d and The Types Of Explants On Callus Inductionmentioning

confidence: 58%

Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema Indicum var. Aromaticum a Scented Chrysanthemum

Jin¹,

Yang²,

Gao³

et al. 2017

JPS

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“…Results of induction of organogenic calli or shoot induction, similar to those reported here were described on cultures using axillary buds of nodal explants in cucurbitaceous Momordica charantia L. (0.5 mg • L -1 BA and 2.0 mg • L -1 NAA) (Agarwal and Kamal, 2007), in hypocotyl and leaf explants of Cucumis anguria L. (0.5 mg • L -1 BA and 1.5 mg • L -1 of 2,4-D, 1.5 mg • L -1 IAA) (Ju et al, 2014) during propagation in Momordica balsamina using 1.0 mg • L -1 BA (Thakur et al, 2011) and callus induction and plantlet regeneration of Citrullus colocynthis (Cucurbitaceae) with 0.5 mg • L -1 IAA, 0.5 mg • L -1 2,4-D, and 1 mg • L -1 BA (Satyavani et al, 2011).…”

Section: Callus Induction and Shoot Regenerationmentioning

confidence: 99%

Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

Arciniega-Carreón

Oliver-Salvador

Ramírez-Sotelo

et al. 2017

horts

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Ibervillea sonorae is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L−1 α-naphthalene-acetic acid (NAA), 0.5 mg·L−1 N6-benzyladenine (BA), and 1.0 mg·L−1 indole-3-acetic acid (IAA) successfully regenerated shoots in I. sonorae explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L−1 indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L−1 IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.

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“…Savitha et al (2010aSavitha et al ( , 2010b, Shasthree et al (2012Shasthree et al ( , 2014 and Ramakrishna and Shasthree (2016) noted that seedling-derived leaves formed callus more than stems (65% vs 45% of explants) while stems formed shoots more than leaves (75% vs 51% of explants). Satyavani et al (2011a) induced shoots indirectly from callus, which was induced from stem explants and a maximum of 20 shoots/explant could be induced. Taha and Mutasher (2014) also found that seedling-derived leaves and stems were effective (induced in 83 (leaves) -85% (stems) of explants) explants for callus induction.…”

Section: Tissue Culture and Genetic Transformationmentioning

confidence: 99%

“…The cryopreservation of C. colocynthis seeds has already provided one such possibility for the long-term preservation of germplasm (Alsadon et al, 2014). Using callus that they had induced from the leaves of C. colocynthis (Satyavani et al, 2011a), Satyavani et al (2011b) produced State, India Similar protocol to Savitha et al 2010aSavitha et al , 2010bShasthree et al 2012Shasthree et al , 2014 Similar protocol to Savitha et al 2010aSavitha et al , 2010bShasthree et al 2012 State, India Similar protocol to Savitha et al 2010aSavitha et al , 2010bShasthree et al 2012Shasthree et al , 2014 Similar protocol to Savitha et al 2010aSavitha et al , 2010bShasthree et al 2012Shasthree et al , 2014 MS+1 mg/L 2,4-D+1.5 mg/L BA (SEIM)…”

Section: Future Perspectivesmentioning

confidence: 99%

Citrullus colocynthis (L.) Schrad. (colocynth): Biotechnological perspectives

Silva¹,

Hussain

2017

Emir. J. Food Agric

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Effect of Plant Growth Regulators on Callus Induction and Plantlet Regeneration of Bitter Apple (Citrullus colocynthis) from Stem Explant

Cited by 31 publications

References 2 publications

Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema Indicum var. Aromaticum a Scented Chrysanthemum

Establishment of Callus Induction and Cell Suspension Cultures of Dendrathema Indicum var. Aromaticum a Scented Chrysanthemum

Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

Citrullus colocynthis (L.) Schrad. (colocynth): Biotechnological perspectives

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