Effect of Porcine Clostridium perfringens on Intestinal Barrier, Immunity, and Quantitative Analysis of Intestinal Bacterial Communities in Mice

Jiang, Zipeng; Su, Weixu; Wen, Chaoyue; Li, Wentao; Gong, Tao; Du, Shuai; Wang, Xinxia; Lu, Zeqing; Jin, Mingliang; Wang, Yizhen

doi:10.3389/fvets.2022.881878

Cited by 10 publications

(8 citation statements)

References 64 publications

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“…Our data indicated that C. perfringens infection shortened the colon length at 1 dpi ( Figure 2C ), suggesting potential colonic inflammation. Interestingly, mice infected with porcine C. perfringens displayed a shortened colon length in an infectious dose-dependent manner ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Natural intraepithelial lymphocyte populations rise during necrotic enteritis in chickens

Majeed,

Hamad,

Shah

et al. 2024

Front. Immunol.

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Intraepithelial lymphocytes (IEL) reside in the epithelium at the interface between the contents of the intestinal lumen and the sterile environment of the lamina propria. Because of this strategic location, IEL play a crucial role in various immunological processes, ranging from pathogen control to tissue stability. In mice and humans, IEL exhibit high diversity, categorized into induced IEL (conventional CD4 and CD8αβ T cells) and natural IEL (TCRαβCD8αα, TCRγδ, and TCRneg IEL). In chickens, however, the subpopulations of IEL and their functions in enteric diseases remain unclear. Thus, we conducted this study to investigate the role of IEL populations during necrotic enteritis (NE) in chickens. At 14 days of age, sixty-three Specific-pathogen-free (SPF) birds were randomly assigned to three treatments: Control (sham challenge), Eimeria maxima challenge (EM), and Eimeria maxima + Clostridium Perfringens (C. Perfringens) co-challenge (EM/CP). The EM and EM/CP birds were infected with Eimeria maxima at day 14 of age, and EM/CP birds were additionally orally inoculated with C. perfringens at days 18 and 19 of age. Birds were weighed at days 18, 20, and 26 of age to assess body weight gain (BWG). At 20 days of age (1 day-post C. perfringens infection; dpi), and 26 days of age (7 dpi), 7 birds per treatment were euthanized, and jejunum was harvested for gross lesion scores, IEL isolation, and gene expression. The EM/CP birds exhibited subclinical NE disease, lower BWG and shorter colon length. The Most changes in the IEL populations were observed at 1 dpi. The EM/CP group showed substantial increases in the total number of natural IEL subsets, including TCRαβ+CD4-CD8-, TCRαβ+CD8αα+, TCRγδ+, TCRneg and innate CD8α (iCD8α) cells by at least two-fold. However, by 7 dpi, only the number of TCRαβ+CD4-CD8- and TCRαβ+CD8αα+ IEL maintained their increase in the EM/CP group. The EM/CP group had significantly higher expression of proinflammatory cytokines (IL-1β and IFN-γ) and Osteopontin (OPN) in the jejunum at 1 dpi. These findings suggest that natural IEL with innate and innate-like functions might play a critical role in the host response during subclinical NE, potentially conferring protection against C. perfringens infection.

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Section: Discussionmentioning

confidence: 99%

Natural intraepithelial lymphocyte populations rise during necrotic enteritis in chickens

Majeed,

Hamad,

Shah

et al. 2024

Front. Immunol.

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“…As our previous study described, we used the Clostridium perfringens to construct a mouse model to test the effect of isolated strains in vivo [ 35 ]. We measured isolated strains’ function by analyzing the growth performance, immune status, and the population of beneficial and harmful bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…We exerted Nanodrop 2000 (Thermo Fisher Scientific Inc., Waltham, MA, USA) to quantify the purified insert-containing plasmids. Then target DNA copies were calculated [ 35 ]:

…”

Section: Methodsmentioning

confidence: 99%

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

Jiang

Yang

et al. 2022

Microorganisms

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Various countries and organizations call for banning the use of antibiotic growth promoters (AGPs) as prophylaxis and for growth promotion in the livestock industry. Hence, seeking a substitute for antibiotics is strongly required by the livestock industry to maintain the productivity level and profits. Probiotics could represent one viable solution because of their beneficial effects on host health and maintaining the intestinal microbiota balance. In the present study, we aimed to isolate bacterial strains with probiotics properties from JinHua pig (a Chinese native pig breed) gastrointestinal tract that have antagonistic activity against to common disease-causing bacteria on farms. The four most potent strains were isolated (PP31, BA11, BA40, BV5) by the agar well diffusion method and further characterized by acid, bile salt, trypsin tolerance, whole genome sequencing (WGS), and suppressing Clostridium perfringens adhesion to IPEC-J2 cells. According to these results, BA40 had the highest number and variety of probiotic secondary metabolic secretion genes and capacity to exclude the attachment of Clostridium perfringens to IPEC-J2 cells as same as PB6. The animal experiment in vivo illustrated that BA40 and PB6 could reduce the phenomenon induced by Clostridium perfringens challenge of body weight loss, colon length decrease, pro-inflammatory cytokine increase, and Clostridium perfringens and Escherichia coli increase. The present study provides evidence that BA40 could represent a novel probiotic candidate as PB6, which exhibited some probiotic features and mitigated the burden of Clostridium perfringens associated gut disease.

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“…1 For animal husbandry, necrotizing enterocolitis linked with C. perfringens is responsible for an estimated economic loss between USD$2 and $6 billion annually. 2 What is worse, the cost for treating necrotizing enterocolitis clinically caused by C. perfringens in preterm infants is conservatively estimated at around £13.5 million in the UK per year. 3 Despite growing scientific concern over antibiotic resistance, a compound of penicillin combined with either clindamycin or a carbapenem is the recommended treatment of choice for this infectious disease.…”

Section: ■ Introductionmentioning

confidence: 99%

“…For animal husbandry, necrotizing enterocolitis linked with C. perfringens is responsible for an estimated economic loss between USD$2 and $6 billion annually . What is worse, the cost for treating necrotizing enterocolitis clinically caused by C.…”

Section: Introductionmentioning

confidence: 99%

G Protein-Coupled Receptor 120 Mediates Host Defense against Clostridium perfringens Infection through Regulating NOD-like Receptor Family Pyrin Domain-Containing 3 Inflammasome Activation

Liu

Lei

et al. 2023

J. Agric. Food Chem.

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Clostridium perfringens is a major cause of infectious foodborne disease, frequently associated with the consumption of raw and undercooked food. Despite intensive studies on clarifying C. perfringens pathogenesis, the molecular mechanisms of host− pathogen interactions remain poorly understood. In soft tissue and mucosal infection models, Gpr120 -/mice, G protein-coupled receptor 120 (GPR120), are more susceptible to C. perfringens infection. Gpr120 deficiency leads to a low survival rate (30 and 10%, p < 0.01), more bacterial loads in the muscle (2.26 × 10 8 ± 2.08 × 10 8 CFUs/g, p < 0.01), duodenum (2.80 × 10 7 ± 1.61 × 10 7 CFUs/g, p < 0.01), cecum (2.50 × 10 8 ± 2.05 × 10 8 CFUs/g, p < 0.01), and MLN (1.23 × 10 6 ± 8.06 × 10 5 CFUs/g, p < 0.01), less IL-18 production in the muscle (8.54 × 10 3 ± 1.20 × 10 3 pg/g, p < 0.01), duodenum (3.34 × 10 3 ± 2.46 × 10 2 pg/g, p < 0.01), and cecum (3.81 × 10 3 ± 5.29 × 10 2 pg/g, p < 0.01), and severe organ injury. Obviously, GPR120 facilitates IL-18 production and pathogen control via potassium efflux-dependent NOD-like receptor family pyrin domain-containing 3 (NLRP3) signaling. Mechanistically, GPR120 interaction with NLRP3 potentiates the NLRP3 inflammasome assembly. Thus, this study uncovers a novel role of GPR120 in host protection and reveals that GPR120 may be a potential therapeutic target for limiting pathogen infection.

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Effect of Porcine Clostridium perfringens on Intestinal Barrier, Immunity, and Quantitative Analysis of Intestinal Bacterial Communities in Mice

Cited by 10 publications

References 64 publications

Natural intraepithelial lymphocyte populations rise during necrotic enteritis in chickens

Natural intraepithelial lymphocyte populations rise during necrotic enteritis in chickens

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

G Protein-Coupled Receptor 120 Mediates Host Defense against Clostridium perfringens Infection through Regulating NOD-like Receptor Family Pyrin Domain-Containing 3 Inflammasome Activation

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