Effect of porcine follicular fluid proteins and peptides on oocyte maturation and their subsequent effect on in vitro fertilization

Ducolomb, Yvonne; González‐Márquez, Humberto; Fierro, Reyna; Jiménez, Irma; Casas, Eduardo; Flores, Diana; Bonilla, Edmundo; Salazar, Zayil; Betancourt, Miguel

doi:10.1016/j.theriogenology.2013.01.024

Cited by 36 publications

(34 citation statements)

References 54 publications

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“…Immunohistochemical studies showed that the levels of transferrin and its receptor in mature follicles were significantly higher than in immature follicles [41], suggesting that endogenous transferrin may play a role in follicular maturation [44]. A previous study demonstrated that transferrin levels were upregulated in FF from the patients with PCOS [35].…”

Section: Discussionmentioning

confidence: 95%

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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Purpose To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. Methods Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes. Protein expression profiles in each FF sample were analyzed respectively using twodimensional gel electrophoresis. Differentially expressed proteins were identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and validated by western blotting. Differentially expressed proteins were further analyzed using Ingenuity Pathway Analysis (IPA) software.Results Two proteins were downregulated and 11 proteins were upregulated (change ≥1.5-fold, P<0.05) in the COH group. We identified one down-egulated and seven upregulated proteins using MALDI-TOF MS. Four differentially expressed proteins, including transferrin, complement component C3 (C3), haptoglobin and alpha-1-antitrypsin (AAT), were further validated by rate nephelometry and western blotting analyses. The IPA analysis revealed a significant network involved in the humoral immune and inflammatory responses. Conclusions The eight differentially expressed proteins were related to immune and inflammatory responses in the ovary. Our results provide new insights into the influence of COH on follicular (spp) development and IVF outcomes.

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Section: Discussionmentioning

confidence: 95%

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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“…It seems counterintuitive to include follicular fluid to encourage oocyte maturation, as it contains oocyte maturation inhibitors, such as hypoxanthine, which arrest oocyte meiosis (30)(31)(32). Nevertheless, the beneficial effects of using follicular fluid during IVM in many studies (33)(34)(35)(36) provided clues that its content of growth factors could be beneficial for oocyte maturation. Here, we provide evidence that three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), each known to be present in follicular fluid (37)(38)(39)(40), when used in combination can provide much improved oocyte competence, most probably by influencing the timing of MAPK1/3 activation in the cumulus cells enveloping the oocyte.…”

Section: Significancementioning

confidence: 99%

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Yuan

Spate

Redel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

117

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Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. Here we show that a chemically defined maturation medium supplemented with three cytokines (FGF2, LIF, and IGF1) in combination, so-called "FLI medium," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovaries and provides a twofold increase in the efficiency of blastocyst production after in vitro fertilization. Transfer of such blastocysts to recipient females doubles mean litter size to about nine piglets per litter. Maturation of oocytes in FLI medium, therefore, effectively provides a fourfold increase in piglets born per oocyte collected. As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte within. These events likely underpin the improvement in oocyte quality achieved by using the FLI medium.cumulus cell | embryo development | genetic modification | in vitro fertilization | MAPK signaling

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“…Follicular fluid as a maturation medium is sufficient to promote nuclear maturation; however, it presents limitations to support appropriate embryo development (Avery et al, 2003;Coleman et al, 2007). Due to the inhibitory effect on germinal vesicle breakdown and on meiotic progression, FF leads to a delay on the nuclear maturation (Ducolomb et al, 2013). In this sense, the identification of the optimal time for fertilization of oocytes matured in FF might improve output and quality of blastocyst.…”

Section: Introductionmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

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-This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 -100% FF) slowed meiotic progression and CG migration (control -78.13% vs. treated -52.58% and control -52.7% vs. treated -11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control -90.13% vs. treated -82.64% and control -35.73% vs. treated -11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control -29.91 vs. treated -35.49, at 24 h of maturation) and total cell numbers (control -109.53 vs. treated -120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.

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Effect of porcine follicular fluid proteins and peptides on oocyte maturation and their subsequent effect on in vitro fertilization

Cited by 36 publications

References 54 publications

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

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