Effect of pore sizes of silk scaffolds for cartilage tissue engineering

Han, Kap-Soo; Song, Jeong Eun; Tripathy, Nirmalya; Kim, Hyeongseok; Moon, Bo Mi; Park, Chan Hum; Khang, Gilson

doi:10.1007/s13233-015-3156-4

Cited by 56 publications

(41 citation statements)

References 27 publications

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“…As reported by Vikingsson et al [51] different pore sizes and wall thickness within the micro-architecture can directly affect the mechanical properties and degradation profile of scaffolds. In a previous study, Han et al [8] observed that the compressive strength of salt-leached/ freeze-dried SF scaffolds produced with aqueous SF solutions of 4-5 wt%, decreased from 1.6 to 1.09 MPa as the pore size increased (90-180 lm to 355-425 lm). This was an expected result, since a lower macro-porosity induce the formation of denser structures that can withstand higher loads.…”

Section: Discussionmentioning

confidence: 96%

“…The clinical use of scaffolds in cartilage regeneration is represented only by a few examples of commercially available products, including the Chondro-Gide Ò (Geistlich Pharma AG, Wolhusen, Switzerland) collagen scaffold that presented promising results for mid-long-term cartilage treatment [6]. The inclusion of interconnected porous-like structures showed to benefit cell adhesion and guided a rapid extracellular matrix (ECM) formation and infiltration within the scaffolds, which represents great advancements on cartilage tissue regeneration, especially when envisioning the use of cell-free strategies [7,8]. On the other hand, different polymeric-based hydrogels have been investigated as injectable systems for arthroscopic insertion approaches [9].…”

Section: Introductionmentioning

confidence: 99%

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Combinatory approach for developing silk fibroin scaffolds for cartilage regeneration

et al. 2018

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In cartilage tissue engineering (TE), several processing technologies have been combined to create scaffolds for efficient tissue repair. In our study, we propose novel silk fibroin (SF) scaffolds derived from enzymatically crosslinked SF hydrogels processed by salt-leaching and freeze-drying technologies, for articular cartilage applications. Though these scaffolds, we were able to combine the elastic properties of hydrogel-based systems, with the stability, resilience and controlled porosity of scaffolds processed via salt-leaching and freeze-drying technologies. SF protein has been extensively explored for TE applications, as a result of its mechanical strength, elasticity, biocompatibility, and biodegradability. Thus, the structural, mechanical and biological performance of the proposed scaffolds potentiates their use as three-dimensional matrices for cartilage regeneration.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Combinatory approach for developing silk fibroin scaffolds for cartilage regeneration

et al. 2018

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“…Silk is biocompatible, biodegradable and has good mechanical properties that because of these features is used in various industries, and also commonly used in the medicine as surgical sutures. Because of high biocompatibility of silk, it can be used as a scaffold for increasing the growth of many stem cells . Moreover, degradation of silk is 6 months in the body and within 2 years is completely decomposed, so it can be used as a good scaffold in tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

“…Because of high biocompatibility of silk, it can be used as a scaffold for increasing the growth of many stem cells. 16 Moreover, degradation of silk is 6 months in the body and within 2 years is completely decomposed, 17 so it can be used as a good scaffold in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Silk nanofibrous electrospun scaffold enhances differentiation of embryonic stem like cells derived from testis in to mature neuron

Bojnordi

Ebrahimi‐Barough

Vojoudi

et al. 2018

J Biomedical Materials Res

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The scaffolds accompanied with stem cells have great potential for applications in neural tissue engineering. Fabrication of nanofibrous scaffold similar to extracellular matrix is one of the applicable methods in neural tissue regeneration. The aim of this study was the fabrication of a silk nanofibrous scaffold as a microenvironment for neural guiding differentiation of embryonic stem like cells (ES Like cells) derived from testis toward neuron-like cells. ES Like derived from culturing of testicular cells in vitro, were seeded on silk scaffolds and induced to neuronal phenotype using 4-/4± RA technique following culturing the cells in the neurobasal medium supplemented with 20 ng/mL bFGF,10 ng/mL EGF, B27, and N2 for 8-12 days. The neural differentiation was confirmed via the evaluation of specific neural markers; Nestin, NF68, MAP2 and β tubulin using immunocytochemistry and real-time polymerase chain reaction. Our results showed that silk scaffold support the attachment and proliferation of ES Like cells. The expression of Nestin, NF68, Map2, and ß tubulin markers were higher in cells grown on silk scaffold in compare to monolayer group. This study suggests electrospun silk nanofibrous scaffold as an appropriate substrate for neural induction of stem cells that is applicable for repairmen of damaged neural tissues. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2662-2669, 2018.

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“…Zhang et al (2010) [12], telah pula mengamati diferensiasi osteogenik dari Bone Morphgenic Protein-7 (BMP-7), dimana diferensiasi osteogenik lebih baik terjadi pada pori berukuran antara 100-300 µm dibandingkan dengan 50-100 µm di dalam scaffold fibroin sutra. Han et al (2015) [13] menambahkan pula bahwa ukuran pori scaffold fibroin sutra 90-250 µm memberi lingkungan terbaik untuk adhesi dan proliferasi kondrosit. Hal tersebut sejalan dengan hasil yang diperoleh pada penelitian ini yaitu ukuran pori sampel Porositas dari scaffold KS-KMS ini telah memenuhi persyaratan dari porositas dasar untuk rekayasa jaringan yaitu sebesar 60% [16].…”

Section: Gambar 1 Spektrum Cmc (Kms) (A) Kitosan (B) Dan Komposit unclassified

Preparasi dan Karakterisasi Kitosan-Karboksi Metil Selulosa Iradiasi untuk Scaffold

Abbas

Warastuti

2018

J. Ilmiah Aplikasi Isotop dan Radiasi

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ABSTRAKScaffold umumnya digunakan sebagai matrik pelepasan obat, penelitian perilaku sel dan material dalam bidang rekayasa jaringan. Scaffold berdimensi tiga (3D) biasanya berupa material berpori, biokompatibel, biodegradable dan berfungsi untuk memberikan lingkungan mikro yang cocok, yaitu dukungan mekanik, fisik, dan rangsangan biokimia untuk pertumbuhan sel optimal. Pada penelitian ini scaffold dibuat dari kitosan dan karboksimetil selulosa (KMS) dengan teknik liofilisasi. Selanjutnya, scaffold diiradiasi dengan sinar gamma dan dikarakterisasi dengan Fourier-Transform-Infrared, Scanning Electron Microscope (SEM), serapan air dan porositas. Hasil yang diperoleh menunjukkan bahwa pada spektrum FTIR diperoleh kedua bahan tersebut tanpa terjadi reaksi, ukuran pori berkisar 130-467 µm, serapan air 803-1722%, dan porositas berkisar 70-74%. Iradiasi mempengaruhi ukuran pori, porositas, dan serapan air. Berdasarkan besaran porinya, komposit ini layak menjadi scaffold.Kata Kunci : komposit, scaffold, kitosan, karboksi meteil selulosa, karakterisasi. ABSTRACTScaffolds are generally used for drug delivery, investigation of cell behavior and material studies in the field of tissue engineering. Three dimensional (3D) scaffolds are typically porous, biocompatible and biodegradable materials that serve to provide suitable microenvironments, that is, mechanical support, physical, and biochemical stimuli for optimal cell growth and function. In this study, the scaffold was prepared from chitosan and carboxy methyl cellulose (CMC) with lyophilization techniques. Furthermore, the scaffold was irradiated with gamma rays and characterized by using Fourier-Transform-Infrared (FTIR) and Scanning Electron Microscope (SEM). It is also detemined water absorption and porosity. The results showed that base on the FTIR spectrum both materials were obtained without reaction, pore size around 130-467, water absorption 803-1722% and porosity around 70-74%. Based on these results, this composite deserves to be a scaffold.

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Effect of pore sizes of silk scaffolds for cartilage tissue engineering

Cited by 56 publications

References 27 publications

Combinatory approach for developing silk fibroin scaffolds for cartilage regeneration

Combinatory approach for developing silk fibroin scaffolds for cartilage regeneration

Silk nanofibrous electrospun scaffold enhances differentiation of embryonic stem like cells derived from testis in to mature neuron

Preparasi dan Karakterisasi Kitosan-Karboksi Metil Selulosa Iradiasi untuk Scaffold

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