Objectives:
The research was carried out on broilers to determine the efficacy of probiotics (
Bacillus subtilis
and
Saccharomyces boulardii
combined) supplementation on growth performances, nutrient retention (metabolizable energy, dry matter, and crude protein), and cecal microbiology (
Bifidobacteria
spp.,
Clostridium
spp., and coliforms).
Materials and Methods:
A total of 160 broiler chicks (day-old) were selected and differentiated randomly into 4 groups (T
0
, T
1
, T
2
, and T
3
) (40 × 4) comprising 40 birds in every single group. The control group (T
0
) was fed commercial broiler feed only and the other three groups, referred to as treatment groups (T
1
, T
2
, and T
3
), were treated with 1 gm ciprofloxacin, 1 gm probiotic, and 1 gm probiotic plus 0.5 gm enzyme, respectively, in per liter of fresh dietary water 8 h daily for 7 days in each phase. Experimental trials were divided into 2 phases, the starter phase from day 0 to 21 and the finisher phase from day 22 to 35.
Results:
Bodyweight gain and nutrient retention in experimental broiler birds in treatment groups were significantly (
p
< 0.05) higher than the control group. Overall body weight gain and nutrient retention of broiler chicks in treatment groups T
2
and T
3
were better than T
1
. From day 22 to 35, cecal
Clostridium
and coliform bacterial load counts were significantly lower
p
< 0.01,
p
< 0.05, and
p
< 0.01, respectively, in T
1
, T
2
, and T
3
treatments than T
0
. Overall,
Clostridium
and coliform bacterial counts in the birds of treatment group T
2
were significantly lower (
p
< 0.05) than T
0
.
Conclusion:
The probiotics, in addition to enzyme supplementation, had suitable influence effects on growth performance of broilers, birds retention of nutrient, and microfloral count in birds’ cecum.