Objective: the present study aimed to evaluate the wettability of different sealers in contact with dentin treated with irrigants used for smear layer removal, after the use of chlorhexidine as chemical auxiliary substance. Material and Methods: thirty single-rooted teeth were used. The crowns were removed and the roots sectioned vertically, resulting in two segments, which were then flattened. Thus, 120 samples measuring 5 mm were obtained. They were immersed in 2% chlorhexidine gel (CHX) for 10 minutes and washed with distilled water (AD) before being subjected to the final irrigant for smear layer removal. The following irrigation protocols were evaluated: control group-CHX+AD+AD+AD; group E-CHX+AD+17%EDTA+AD; group Q-CHX+AD+QMix+AD; group Et-CHX+AD+18%HEBP+AD. The samples were dried and the contact angle was analyzed using a goniometer. The sealers evaluated were: AH Plus (AH), Pulp Canal Sealer EWT (PCS) and MTA Fillapex (MTAF). In each drop of sealer, 60 measurements were made and the flow was observed for 1 minute. The dynamic wettability of the sealers was calculated. Data were statistically analyzed. Results: in all sealers, the presence of smear layer disfavored the wettability. EDTA and QMix favored the wettability of AH and PCS. For the MTAF, QMix showed the best performance, followed by EDTA. HEBP showed statistically lower results, similar to the control. Conclusion: Solutions used for smear layer removal, after the use of chlorhexidine during preparation, influenced the wettability of AH Plus, Pulp Canal Sealer EWT and MTA Fillapex sealers.