Effect of Quercetin on Lipids Metabolism Through Modulating the Gut Microbial and AMPK/PPAR Signaling Pathway in Broilers

Abstract

The present study was conducted to investigate effects and mechanism of quercetin on lipids metabolism in broilers. 480 AA broilers were randomly allotted to four treatments (0, 0.2, 0.4, and 0.6 g/kg quercetin) for 42 days. Compared with the control, 0.6 g/kg quercetin significantly decreased percentage of abdominal fat (P < 0.05); 0.2, 0.4, and 0.6 g/kg quercetin significantly decreased relative abundance of Lachnospiraceae and Desulfovibrionaceae (P < 0.05, P < 0.05, P < 0.01; P < 0.01, P < 0.01, P < 0.01); 0.2 g/kg quercetin significantly increased mRNA expression of PI3K, AMPKα1, AMPKα2, AMPKβ2, LKB1 (P < 0.01, P < 0.01, P < 0.05, P < 0.01, P < 0.05), and significantly reduced mRNA expression of SREBP1 and PPARγ (P < 0.01, P < 0.05); 0.4 g/kg quercetin significantly increased mRNA expression of LKB1 and PKB (P < 0.05, P < 0.01) and significantly reduced mRNA expression of ACC, HMGR, PPARγ, and SREBP1 (P < 0.05, P < 0.01, P < 0.01, P < 0.01); 0.6 g/kg quercetin significantly increased mRNA expression of AMPKγ, LKB1, CPT1, PPARα, PKB (P < 0.01, P < 0.01, P < 0.01, P < 0.05, P < 0.05), and significantly reduced the mRNA expression of PI3K, ACC, HMGR, PPARγ, SREBP1 (P < 0.05, P < 0.05, P < 0.01, P < 0.01, P < 0.01); 0.2 g/kg quercetin significantly increased protein expression of AMPK (P < 0.01); 0.6 g/kg quercetin significantly increased protein expression of LKB1 (P < 0.01), 0.2 and 0.6 g/kg quercetin significantly increased protein expression of PI3K, PKB, CPT1 (P < 0.05, P < 0.01, P < 0.05, P < 0.01, P < 0.01, P < 0.01), and significantly reduced protein expression of ACC and SREBP1 (P < 0.01, P < 0.01, P < 0.01, P < 0.01). In conclusion, quercetin improved lipid metabolism by modulating gut microbial and AMPK/PPAR signaling pathway in broilers.

Quercetin extracted from Sophora japonica flower improves growth performance, nutrient digestibility, cecal microbiota, organ indexes, and breast quality in broiler chicks

“…The lipid deposition regulation properties of quercetin are achieved by modulating the lipid synthesized in hepatocytes [ 38 ]. Quercetin could reduce lipid deposition in broiler chicks by promoting PPARα-regulated lipid decomposition [ 38 ], reducing the expression levels of sterol regulatory element-binding protein 1, peroxisome proliferator-activated receptor gamma, and 3-hydroxy-3-methylglutaryl-CoA reductase in the liver [ 39 ], and activating P13K/PKB, AMRK, and/or cAMP signal pathway [ 39 – 41 ]. It has been reported that quercetin supplementation reduced the increase of adipose tissue weight induced by feeding with a high-fat diet in rats [ 32 , 42 , 43 ].…”

“…Quercetin could reduce lipid deposition in broiler chicks by promoting PPARα-regulated lipid decomposition [38], reducing the expression levels of SREBP1, PPARγ, and HMGR in the liver [39], and activating P13K/PKB, AMRK, and/or cAMP signal pathway [39][40][41]. It has been reported that quercetin supplementation reduced the increase of adipose tissue weight induced by feeding with a high-fat diet in rats [32,42,43].…”

Hypericum lanceolatum Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model

“…It has been reported that H. perforatum L. aqueous extract (which has the same plant taxonomy as H. lanceolatum ) significantly reduced serum levels of triglycerides, LDL-C (low density lipoprotein cholesterol) as well as oxidative stress biomarkers induced by hyperlipidemia in rats ( Zou et al, 2005 ; Ghosian Moghaddam et al, 2016 ). In addition, supplementation with chlorogenic acid and quercetin has been shown to modulate the expression of some lipogenesis- and lipolysis-related genes, respectively, in mice and chicken after a HFD ( Wang et al, 2019 ; Wang et al, 2021 ). The authors also suggested that the prevention of obesity-related disturbances might be closely associated with the modulation of the gut microbiota ( Wang et al, 2019 ; Wang et al, 2021 ).…”

A System-Level Mechanism of Anmyungambi Decoction for Obesity: A Network Pharmacological Approach

“…Also, limonin in PC inhibits upregulated phosphorylation of PKA, resulting in modulating the cAMP signaling pathway [ 51 ]. Moreover, quercetin suppressed the protein expression levels of PI3K [ 52 , 53 , 54 ] Our findings suggest that AMGB stimulates lipolysis in adipocytes mediating the cAMP signaling pathway and related proteins.…”