Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [ 3 H]noradrenaline from a slice preparation

Borbély, Zoltán; Csomó, Benedek Krisztián; Kittel, Ágnes; Gerber, Gábor; Varga, Gábor; Vízi, E. S.

doi:10.1016/j.brainresbull.2017.04.007

Cited by 17 publications

(5 citation statements)

References 46 publications

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“…Spinal cord morphology is of great importance for establishing the type of spinal cord injury, especially bilateral or unilateral contusion and hemisection models [32][33][34][35][36]. More precise parameters are needed to generate, with high repeatability, a unilateral contusion model that enables further study of limb function and a higher survival rate [12,37,38].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the anatomical morphology of cervical vertebrae between humans and macaques: related to a spinal cord injury model

Liu

Zhou

et al. 2021

Exp. Anim.

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Non-human primates are most suitable for generating cervical experimental models, and it is necessary to study the anatomy of the cervical spine in non-human primates when generating the models. The purpose of this study was to provide the anatomical parameters of the cervical spine and spinal cord in long-tailed macaques (Macaca fascicularis) as a basis for cervical spine-related experimental studies.Cervical spine specimens from 8 male adult subjects were scanned by micro-computed tomography, and an additional 10 live male subjects were scanned by magnetic resonance imaging. The measurements and parameters from them were compared to those of 12 male adult human subjects. Additionally, 10 live male subjects were scanned by magnetic resonance imaging, and the width and depth of the spinal cord and spinal canal and the thickness of the anterior and posterior cerebrospinal fluid were measured and compared to the relevant parameters of 10 male adult human subjects. The tendency of cervical parameters to change with segmental changes was similar between species. The vertebral body, spinal canal, and spinal cord were significantly flatter in the human subjects than in the long-tailed macaques.The cerebrospinal fluid space in the long-tailed macaques was smaller than that in the human subjects.The anatomical features of the cervical vertebrae of long-tailed macaques provide a reference for establishing a preclinical model of cervical spinal cord injury.

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Section: Discussionmentioning

confidence: 99%

Comparison of the anatomical morphology of cervical vertebrae between humans and macaques: related to a spinal cord injury model

Liu

Zhou

et al. 2021

Exp. Anim.

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“…Nevertheless, it is clear that the pathophysiology of the development and progress of PHN includes both central and peripheral disorders of the nervous system. Neuronal transmission is achieved by a wide range of neurotransmitters and regulatory peptides such as acetylcholine [ 42 ], noradrenaline [ 43 ], ATP [ 44 ], nitric oxide [ 45 ], GABA [ 46 ], cholecystokinin [ 47 ], gastrin [ 48 ], GRP [ 49 ], VIP [ 50 ], galanin [ 51 ], bombesin [ 52 ] and BPC 157 [ 53 ]. Among these, GABA seems to be very important in pain control, because of the descending GABA-ergic neuronal nerve fibres inhibiting peripheral nociceptive signals [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

γ-Aminobutyric Acid and Derivatives Reduce the Incidence of Acute Pain after Herpes Zoster - A Systematic Review and Meta-analysis

Sadaeng

Korbonits

Mátrai

et al. 2020

CPD

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: Herpes zoster (HZ) causes considerable pain and distress, and γ-Aminobutyric acid (GABA) and its derivatives are assumed to control this, but the available data are inconsistent. This metaanalysis and systematic review aimed to assess the effectiveness of GABA derivatives in the prevention of acute herpetic pain. The meta-analysis was conducted following the PRISMA guidelines using PICO format, registered in PROSPERO number CRD42018095758. PubMed, Web of Science, Ovid, Scopus, and EMBASE databases were searched. Records were included if they were randomized controlled trials of patients undergoing HZ infection, investigating the effect of GABA derivatives versus placebo in the treatment of HZ pain. Eligible trials were evaluated for the risk of bias. Then data were extracted and analysed. The number of patients with observed presence of pain after treatment was used to calculate odds ratio in a random effect model with the DerSimonian-Laird estimator. The I 2 statistic was analysed for heterogeneity. The potential risk of bias was measured using Egger’s regression test. The metaanalysis included three randomized controlled trials with a total of 297 patients. The incidence of acute HZ pain events for GABA group was significantly lower compared to placebo group,18/148 vs 44/159, respectively (OR = 0.36; 95% CI = 0.14 to 0.93; Z = 2.11; P = 0.035), Egger’s test yielded P = 0.308. In conclusion, the present meta-analysis demonstrates that GABA derivatives reduce the incidence of acute herpetic pain. However, additional, well-designed randomized clinical trials are needed to determine their dose- and time-dependency regarding this symptom.

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“…The striatum or frontal cortex was dissected and cut transversely into 400-µm slices using a McIlwain tissue slicer. The slice preparations used in this study were previously described [60]. In our experiments, we did not compensate for the changes in osmolarity produced by the removal of glucose because we did not observe any differences in the release rates when glucose was not replaced [61].…”

Section: Preparation Of Rat Striatal and Cortical Slicesmentioning

confidence: 99%

Roles Played by the Na+/Ca2+ Exchanger and Hypothermia in the Prevention of Ischemia-Induced Carrier-Mediated Efflux of Catecholamines into the Extracellular Space: Implications for Stroke Therapy

et al. 2019

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The release of [ 3 H]dopamine ([ 3 H]DA) and [ 3 H]noradrenaline ([ 3 H]NA) in acutely perfused rat striatal and cortical slice preparations was measured at 37 °C and 17 °C under ischemic conditions. The ischemia was simulated by the removal of oxygen and glucose from the Krebs solution. At 37 °C, resting release rates in response to ischemia were increased; in contrast, at 17 °C, resting release rates were significantly reduced, or resting release was completely prevented. The removal of extracellular Ca 2+ further increased the release rates of [ 3 H]DA and [ 3 H]NA induced by ischemic conditions. This finding indicated that the Na + /Ca 2+ exchanger (NCX), working in reverse in the absence of extracellular Ca 2+ , fails to trigger the influx of Ca 2+ in exchange for Na + and fails to counteract ischemia by further increasing the intracellular Na + concentration ([Na + ] i ). KB-R7943, an inhibitor of NCX, significantly reduced the cytoplasmic resting release rate of catecholamines under ischemic conditions and under conditions where Ca 2+ was removed. Hypothermia inhibited the excessive release of [ 3 H] DA in response to ischemia, even in the absence of Ca 2+ . These findings further indicate that the NCX plays an important role in maintaining a high [Na + ] i , a condition that may lead to the reversal of monoamine transporter functions; this effect consequently leads to the excessive cytoplasmic tonic release of monoamines and the reversal of the NCX. Using HPLC combined with scintillation spectrometry, hypothermia, which enhances the stimulation-evoked release of DA, was found to inhibit the efflux of toxic DA metabolites, such as 3,4-dihydroxyphenylacetaldehyde (DOPAL). In slices prepared from human cortical brain tissue removed during elective neurosurgery, the uptake and release values for [ 3 H]NA did not differ from those measured at 37 °C in slices that were previously maintained under hypoxic conditions at 8 °C for 20 h. This result indicates that hypothermia preserves the functions of the transport and release mechanisms, even under hypoxic conditions. Oxidative stress (H 2 O 2 ), a mediator of ischemic brain injury enhanced the striatal resting release of [ 3 H]DA and its toxic metabolites (DOPAL, quinone). The study supports our earlier findings that during ischemia transmitters are released from the cytoplasm. In addition, the major findings of this study that hypothermia of brain slice preparations prevents the extracellular calcium concentration ([Ca 2+ ] o )-independent non-vesicular transmitter release induced by ischemic insults, inhibiting Na + /Cl − -dependent membrane transport of monoamines and their toxic metabolites into the extracellular space, where they can exert toxic effects.

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Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [ 3 H]noradrenaline from a slice preparation

Cited by 17 publications

References 46 publications

Comparison of the anatomical morphology of cervical vertebrae between humans and macaques: related to a spinal cord injury model

Comparison of the anatomical morphology of cervical vertebrae between humans and macaques: related to a spinal cord injury model

γ-Aminobutyric Acid and Derivatives Reduce the Incidence of Acute Pain after Herpes Zoster - A Systematic Review and Meta-analysis

Roles Played by the Na+/Ca2+ Exchanger and Hypothermia in the Prevention of Ischemia-Induced Carrier-Mediated Efflux of Catecholamines into the Extracellular Space: Implications for Stroke Therapy

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