Effect of Resveratrol on biofilm formation and virulence factor gene expression of <i>Porphyromonas gingivalis</i> in periodontal disease

Kugaji, Manohar; Kumbar, Vijay; Peram, Malleswara Rao; Patil, Sanjivini; Bhat, Kishore; Diwan, Prakash V.

doi:10.1111/apm.12930

Cited by 59 publications

(48 citation statements)

References 38 publications

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“…which may act concomitantly to provide differential pathogenic abilities to the P. gingivalis organism. Recently, studies on periodontitis have been focused on the inhibition of the virulence potential of P. gingivalis by different mechanisms to provide health benefits in these subjects [26,27].The variable pathogenic ability due to fimbrial occurrences could also lead us to organize primary and secondary prevention systems and suggests the use of fimbrial proteins, such as fimbrillins in immunoprophylaxis or immunotherapeutic strategies, to eliminate this pathogen from the subgingival microbiota.…”

Section: Discussionmentioning

confidence: 99%

Variation in the Occurrence of fimA Genotypes of Porphyromonas gingivalis in Periodontal Health and Disease

Kugaji¹,

Muddapur

Bhat³

et al. 2020

IJERPH

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Porphyromonas gingivalis is regarded as a “keystone pathogen” in periodontitis. The fimbria assists in the initial attachment, biofilm organization, and bacterial adhesion leading to the invasion and colonization of host epithelial cells. The present study aimed to investigate the occurrence of fimA genotypes in patients with chronic periodontitis and healthy individuals in the Indian population, and to study their association with the number of P. gingivalis cells obtained in subgingival plaque samples of these subjects. The study comprised 95 samples from the chronic periodontitis (CP) group and 35 samples from the healthy (H) group, which were detected positive for P. gingivalis in our previous study. Fimbrial genotyping was done by PCR and PCR-restriction fragment length polymorphism (RFLP). The fimA type II was more prevalent in the CP group (55.89%), followed by type IV (30.52%), whereas in the H group, type I was the most prevalent fimbria (51.42%). The quantity of P. gingivalis cells increased with the presence of fimA types II and III. Our results suggest a strong relationship between fimA types II and IV and periodontitis, and between type I and the healthy condition. The colonization of organisms was increased with the occurrence of type II in deep periodontal sites, which could play an important role in the progression of the disease.

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Section: Discussionmentioning

confidence: 99%

Variation in the Occurrence of fimA Genotypes of Porphyromonas gingivalis in Periodontal Health and Disease

Kugaji¹,

Muddapur

Bhat³

et al. 2020

IJERPH

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“…Some polyphenols and flavonoids present in wine and tea are known to inhibit gingipain activity and interfere with biofilm formation by P. gingivalis in gingival cells models [ 124 ]. Resveratrol has shown significant results by targeting inflammatory and adhesive markers, and also attenuated the virulence of P. gingivalis by reducing the expression of virulence factor genes such as fimbriae (Type II and IV) and proteinases (kgp and rgpA) [ 125 ].…”

Section: Microbiome Modulation By Diet/wine Polyphenols and Alzheimentioning

confidence: 99%

Relationship between Wine Consumption, Diet and Microbiome Modulation in Alzheimer’s Disease

et al. 2020

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Alzheimer’s disease (AD) is a progressive neurodegenerative disorder leading to the most common form of dementia in elderly people. Modifiable dietary and lifestyle factors could either accelerate or ameliorate the aging process and the risk of developing AD and other age-related morbidities. Emerging evidence also reports a potential link between oral and gut microbiota alterations and AD. Dietary polyphenols, in particular wine polyphenols, are a major diver of oral and gut microbiota composition and function. Consequently, wine polyphenols health effects, mediated as a function of the individual’s oral and gut microbiome are considered one of the recent greatest challenges in the field of neurodegenerative diseases as a promising strategy to prevent or slow down AD progression. This review highlights current knowledge on the link of oral and intestinal microbiome and the interaction between wine polyphenols and microbiota in the context of AD. Furthermore, the extent to which mechanisms bacteria and polyphenols and its microbial metabolites exert their action on communication pathways between the brain and the microbiota, as well as the impact of the molecular mediators to these interactions on AD patients, are described.

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“…After revival, these bacterial broths were used as direct colony suspensions, to prepare secondary bacterial aliquots by mixing into BHI broth. The turbidity of each secondary aliquot was adjusted visually to 0.5 McFarland standards and confirmed for all the isolates, by spectrophotometer (Orion™, Aqua-Mate 8000 UV-Vis, Thermo-Fisher Scientific, US) at an optical density (OD600:0.6-0.7), comprising 1x10 7 colony forming units (CFU)/ml [18].…”

Section: Preparation Of Microbial Suspensionmentioning

confidence: 99%

“…The lowest concentration of antimicrobial agent showing no bacterial growth was considered as MIC of the agent for that particular bacteria. All the procedures were repeated in triplicates to minimize errors [18].…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) Of Amentioning

confidence: 99%

Antimicrobial efficacy of a new tri-antibiotic combination against resistant endodontic pathogens: An in-vitro study

Dahake

Baliga

2020

BDS

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Background: Removal of all the pathogenic bacteria from the root canal system is of prime importance for the success of endodontic therapy. Objective: The study aimed to determine the antimicrobial efficacy of three antibiotics and their new combination against selected endodontic pathogens. Methods: In this in-vitro study, we used bacterial strains associated with the refractory endodontic condition and determined MIC and MBC of Clindamycin (C), Metronidazole (M), Doxycycline (D) as well as their combination CMD. We cultured Candida Albicans, Pseudomonas Aeruginosa, Escherichia Coli, Enterococcus Faecalis, Streptococcus Mutans, Bacillus Subtilis subsp. spizizenii, Actinomyces Actinomycetemcomitans on selective culture media. We analyzed the data using paired 't' test, one-way ANOVA, and Tuckey's HSD post hoc test. Results: Clindamycin inhibited the growth of C. Albicans (90%) and S. Mutans (90%) significantly and P. Aeruginosa, E. Coli, E. Faecalis, B. Subtilis, and A. Actinomycetemcomitans were resistant to it. Metronidazole did not inhibit any of the bacteria. Doxycycline inhibited C. Albicans (90%), P. Aeruginosa (90%), and S. Mutans (90%) significantly while E. Coli, E. Faecalis, B. Subtilis, and A. Actinomycetemcomitans were resistant to it. The combination of CMD inhibited all the microbes significantly. However, at bactericidal concentrations of CMD, E. Faecalis (p = 0.024), B. Subtilis (p = 0.021) and A. Actinomycetemcomitans (p = 0.041) were eliminated significantly, while C. Albicans (p = 0.164), P. Aeruginosa (p = 0.489), E. Coli (p = 0.106) and S. Mutans (p = 0.121) showed resistance. Conclusion: Combination CMD can be used against resistant endodontic pathogens to achieve predictable endodontic results.KEYWORDSAntimicrobial agents; Clindamycin; Doxycycline; Metronidazole; Root canal therapy.

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Effect of Resveratrol on biofilm formation and virulence factor gene expression of Porphyromonas gingivalis in periodontal disease

Cited by 59 publications

References 38 publications

Variation in the Occurrence of fimA Genotypes of Porphyromonas gingivalis in Periodontal Health and Disease

Variation in the Occurrence of fimA Genotypes of Porphyromonas gingivalis in Periodontal Health and Disease

Relationship between Wine Consumption, Diet and Microbiome Modulation in Alzheimer’s Disease

Antimicrobial efficacy of a new tri-antibiotic combination against resistant endodontic pathogens: An in-vitro study

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