Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (Centella asiatica (L.) Urban) cells

Abstract

In this study, we report the expression level of CaSQS, CabAS and CaCYS, the genes involved in phytosterol and triterpene metabolic pathway of centella (Centella asiatica (L.) Urban), in cells elicited with salicylic acid (50–200 µM). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis indicated CaSQS, CabAS, and CaCYS genes expressed in both the wild-type and cultured cells (with and without elicitation). In elicited cells, expressions of CaSQS, CabAS, and CaCYS genes showed strong dependence on salicylic acid concentration and elicitation day. The highest expression of CabAS gene was found in the cells elicited with 100 µM salicylic acid on day 10 of inoculation. Salicylic acid treatment (50–200 µM) decreased expression level of CaCYS and CaSQS genes in elicited cells compared with the control.

Accumulation of madecassoside - a major component of centelloside - in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid

“…The results of the present study are consistent with previous studies on the stimulatory effect of SA on asiaticoside biosynthesis (17) and the expression of genes (CaSQS, CabAS and CaCYS) associated with the isoprenoid pathway in centella cells (23). CabAS gene coding for b-amyrin synthase, a key enzyme in biosynthesis of triterpene saponin (madecassoside is a member of this secondary compound group), was more strongly expressed in the cells elicited with 100 µM SA on day 10 after inoculation than in the non-elicited control (23).…”

“…CabAS gene coding for b-amyrin synthase, a key enzyme in biosynthesis of triterpene saponin (madecassoside is a member of this secondary compound group), was more strongly expressed in the cells elicited with 100 µM SA on day 10 after inoculation than in the non-elicited control (23). Study of Wen et al (19) in grape berry (Vitis vinifera L. cv.…”

Salicylic acid‐enhanced biosynthesis of pharmacologically important lignans and neo lignans in cell suspension culture of Linum ussitatsimum L

“…In the literature, the SA has been reported to have encouraging effects on phenolic contents . The enhanced accumulation of TPC might be attributed to the stimulation of PAL activities and metabolic pathways . Yielding, a cascade of high branch phenols are produced .…”

“…Salicylic acid (SA) is a potent plant signaling mol e cule involved in growth responses and providing resistance against pathogens . SA induces defense mechanism via production of compounds involved in defense .…”

“…SA induces defense mechanism via production of compounds involved in defense . When applied exogenously, it mimics the pathogen interaction and prompt the expression of genes and enzymes involved in biosynthesis and catalyzing of phenylpropanoid metabolites . SA as an abiotic elicitor has been widely used to enhance the BAIs in plants cell culture systems .…”