Effect of seasonal acclimatization on the expresion of the carp transcription factor Pit‐1

Kausel, Gudrun; Vera, María; Figueroa, Jaime; Hernández, Jerónimo; Martin, R. H.; Molina, Alfredo; Chavez, Viviana Marcela; Müller, Marc; Martial, Joseph; Krauskopf, Manuel

doi:10.1080/15216549800203242

Cited by 7 publications

(21 citation statements)

References 5 publications

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“…To assess whether the remarkable rise in PRL mRNA and protein, which occurs in summer-acclimatized carp, was associated with a concomitant increase of Pit-1 expression in lactotrophs, we performed in situ hybridization assays using a probe derived from a carp Pit-1 genomic clone containing the 3Ј region of the third exon [Kausel et al, 1998]. Although Pit-1 mRNA levels were significantly higher in the PPD and PI from summer-acclimatized carp compared with winter-adapted fish, singularly, in both seasons only a basal Pit-1 mRNA content was observed in the RPD.…”

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confidence: 99%

Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp

Kausel

Vera²,

Martin

et al. 1999

J. Cell. Biochem.

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A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer-acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer-acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.

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confidence: 99%

Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp

Kausel

Vera²,

Martin

et al. 1999

J. Cell. Biochem.

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“…The habitat of the carp (Cyprinus carpio) undergoes seasonal changes that compel the fish to develop adaptive strategies for sensing the cyclical fluctuation of the physical parameters (e.g. temperature, photoperiod) and to transduce them into molecular signals through mechanisms that involve the neuroendocrine system (Figueroa et al, 1994;Kausel et al, 1998;López et al, 2001). One of the most outstanding cellular features of this adaptive process is the reorganization of the nucleolus architecture.…”

Section: Introductionmentioning

confidence: 99%

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

Vera¹,

Molina²,

Pinto

et al. 2003

Biol. Res.

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The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression of the tandem organized ribosomal genes are located in the non-transcribed intergenic spacer (IGS), we analyzed the primary structure of the carp rDNA gene IGS. The gene organization is similar to that described from other vertebrate species, including numerous repetitive sequences, the transcription start site, and some potential cis-elements such as ribosomal enhancers, proximal terminator and transcriptional terminators. Ribosomal DNA is a remarkable case of gene duplication and has been used as a model to test the concerted evolution theory. We performed sequence comparison analyses of 18S rRNA coding sequences from carp with different species, data with which an unrooted phylogram was constructed.

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“…Classical marker genes for endocrine disrupting and heavy metal ion contamination are, respectively, vg and mt expression in liver of male fish. Recently, the pituitary was identified as a central regulatory organ, at the interface between the central nervous system and the endocrine system, to respond to environmental stimuli both during seasonal acclimatization and upon pollution (Figueroa et al, 1994(Figueroa et al, , 2002(Figueroa et al, , 2005Kausel et al, 1998Kausel et al, , 2010Valenzuela et al, 2012).…”

Section: Discussionmentioning

confidence: 96%

Differential response of two somatolactin genes to zinc or estrogen in pituitary of Cyprinus carpio

Valenzuela

Perez

Navarro

et al. 2015

General and Comparative Endocrinology

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Effect of seasonal acclimatization on the expresion of the carp transcription factor Pit‐1

Cited by 7 publications

References 5 publications

Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp

Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

Differential response of two somatolactin genes to zinc or estrogen in pituitary of Cyprinus carpio

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