Effect of Silk in Silk/PLGA Hybrid Films on Attachment and Proliferation of Human Aortic Endothelial Cells

Abstract

The vascular endothelial cells are the inner layers of blood vessels. It regulates the function of blood vessels and proliferation of vascular smooth muscle cells. Poly(lactide-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with a well-controlled degradation rate and an acceptable mechanical strength. It can be easily fabricated into many shapes. Silk consists of 18 amino acids. It found important for attaching cells cultured in vitro, and maintaining cell functions. In this study, we fabricated silk/PLGA biomaterial hybrid films of 0, 10, 20, 40 and 80 wt% silk. We performed MTT, SEM, ELISA, and immunocytochemistry analyses. We confirmed the adhesion and the proliferation of HAECs on silk/PLGA according to the content of silk, and 40 wt% silk/PLGA hybrid films have superior adhesion and proliferation properties. These results demonstrate that silk/PLGA hybrid films provide suitable surfaces for HAECs, and there is the effect of silk on cell growth and proliferation.

Fabrication and characterization of hydrocolloid dressing with silk fibroin nanoparticles for wound healing

“…Silk fibroin (SF) is a natural polymer composed of 18 amino acids and is particularly rich in glycine, alanine, tyrosine, and serine compared to the amino acids that compose collagen in skin [1][2][3]. Therefore, silk has the advantage of being more absorbent and waterproof compared to other polymers.…”

Hydrophilization of synthetic biodegradable polymer scaffolds for improved cell/tissue compatibility

“…Coated hydrophilic polymers may promote adsorption of ECM molecules as an intermediator between the polymer surface and ECM molecules, and thus can improve cell affinity [101,102]. In addition, numerous ECM molecules, such as collagen [96,[103][104][105], fibronectin [106][107][108][109][110][111], vitronectin [106,112], laminin [104,[113][114][115][116], fibrin [117,118], silk fibroin [119] and short peptide sequences (RGD [arginine-glycine-aspartate], etc) [120] have been coated directly onto the scaffold to improve cell/tissue compatibility.…”