Effect of smoking on the proliferation capacity and osteogenic potential of human dental pulp stem cells (DPSCs)

Kamel, Amany Hany Mohamed; Kamal, Samia; AbuBakr, Nermeen

doi:10.17219/dmp/113179

Cited by 8 publications

(10 citation statements)

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“…hDPSCs is capable to be strongly proliferated and differentiated ( 6 ). In addition, their clinical application can be attributed to their simple and convenient isolation and culture, lack of ethical controversy and low immuno-genicity, which therefore make them as the therapeutic target for the application as autogenous transplantation for pulp and periodontal tissue regeneration ( 7 , 8 ). However, the regulation mechanism of hDPSCs differentiation is still unclear and needed more researches.…”

Section: Discussionmentioning

confidence: 99%

“…Human dental pulp stem cells (hDPSCs), a type of fibroblast and derived from mesenchymal tissue, are regarded to be ideal seed cells for the therapy of pulp regeneration owing to their multidirectional differentiation potential ( 7 , 8 ). hDPSCs could differentiate into osteoblasts, nerve cells, adipocytes, and odontoblasts with great ability to proliferate and differentiate ( 3 , 6 , 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

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MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Huang

2021

IJSC

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Background and Objectives MiR-148a-3p has been reported to regulate the differentiation of marrow stromal cell osteoblast. In this study, whether miR-148a-3p regulated the odontoblastic differentiation of human dental pulp stem cells (hDPSCs) or not was explored. Methods and Results The hDPSCs were isolated and identified via flow cytometry. Targets of miR-148a-3p were identified via bioinformatics and dual-luciferase reporter assay. After the cell was cultured in the odontogenic differentiation medium or infected, cell viability, invasion, and odontoblastic differentiation were detected via MTT, transwell, and Alizarin Red S staining, respectively. The miR-148a-3p, Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions were determined by RT-qPCR and Western blot. The hDPSCs odontoblastic differentiation downregulated the miR-148a-3p expression and upregulated Wnt1 expression. Wnt1 was determined as the target for miR-148a-3p. MiR-148a-3p mimic and siWnt1 suppressed the cell viability, invasion, and odontoblastic differentiation of hDPSCs and inhibited the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. In contrast, miR-148a-3p inhibitor and overexpressed Wnt1 promoted the cell viability, invasion, and odontoblastic differentiation of hDPSCs, and upregulated the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. Also, miR-148a-3p mimic and inhibitor reversed the effects of Wnt1 overexpression and siWnt1. Conclusions MiR-148a-3p modulated the invasion and odontoblastic differentiation of hDPSCs through the Wnt1/β-catenin pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Huang

2021

IJSC

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“…Las células madre mesenquimales (CMM) son el fundamento de la ingeniería tisular y medicina regenerativa de los últimos años, debido a sus capacidades de auto renovación, de diferenciación en múltiples linajes, producción de citocinas y factores de crecimiento que aumentan la dinámica celular lo que genera una capacidad regenerativa y reparativa deseable para su utilización clínica. [1][2][3] Además de estas capacidades, se describe en la literatura el potencial de migración celular, lo que les permite ir al sitio de la lesión de manera guiada y directa para promover la cicatrización, esto sumado a la liberación de factores quimiotácticos y pro-angiogénicos que aumentan la capacidad de cicatrización tisular. 3,4 En la cavidad oral se describen variadas zonas de donde se pueden aislar CMM, tales como: el hueso alveolar, ligamento periodontal, pulpa dental, folículo dentario, papila apical, dientes deciduos exfoliados, mucosa oral y encía.…”

Section: Introductionunclassified

Effect of diabetes on stem cells of the oral cavity. A brief review

Cifuentes-Suazo¹,

Stange-Dempster²,

Muñoz-Diaz³

et al. 2022

RevFacOdont

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Tissue engineering is one of the pillars of regeneration techniques in medicine, based on the use of mesenchymal stem cells (MSCs). MSCs have self-renewal and differentiation capabilities in multiple lines, due to which they are multipotent mature cells. MSCs can be spread over several tiles, a good amount of MSCs is in the oral cavity, including parts such as the alveolar bone, periodontal ligament, dental pulp, dental follicle, oral mucosa and gums. Despite being a major advance in medical sciences, the use of MSCs may be limited by several factors that affect the biological capabilities of these cells. Considering the oral cavity as one of the main sources of obtaining a high amount MSCs, one of the factors that has great repercussions on oral health and diabetes, the clearest example is the two-way relationship that has periodontitis today. periodontitis is considered the sixth complication of diabetes. Given the above, it is necessary to identify the effects thatcould also have on the MSCs obtained in the oral cavity, effects that could hinder their use in tissue engineering. The purpose of this review is to describe the effect of diabetes on MSCs in the oral cavity.

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“…Existen diversos tipos de células madres (CM), se ha descrito que todas poseen capacidad de autorrenovación y capacidad de diferenciación en los tejidos adultos. Las CM mesenquimales (CMM) han tomado un rol importante en la ingeniería tisular y medicina regenerativa, debido a su capacidad de sintetizar citocinas y factores de crecimiento que aumentan la dinámica celular local, lo cual les dan un alto potencial de regeneración y reparación de tejidos 1,2 . Además de su efecto inmunomodulador, las CMM liberan factores proangiogénicos y quimiotácticos, los cuales juegan un papel esencial en la cicatrización de heridas, remodelación ósea y regeneración tisular 2 .…”

Section: Introductionunclassified

“…Las CM mesenquimales (CMM) han tomado un rol importante en la ingeniería tisular y medicina regenerativa, debido a su capacidad de sintetizar citocinas y factores de crecimiento que aumentan la dinámica celular local, lo cual les dan un alto potencial de regeneración y reparación de tejidos 1,2 . Además de su efecto inmunomodulador, las CMM liberan factores proangiogénicos y quimiotácticos, los cuales juegan un papel esencial en la cicatrización de heridas, remodelación ósea y regeneración tisular 2 . Además de tener la capacidad de autorenovación, las CMM tienen la capacidad de migración celular.…”

Section: Introductionunclassified

Effect of tobacco on oral cavity stem cells. A brief review

Cifuentes-Suazo¹,

Stange-Dempster²,

Cartes-Velásquez³

2021

RevFacOdont

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In recent years, mesenchymal stem cells (MSCs) have become the cornerstone of tissue engineering and regenerative medicine. MSCs can be easily obtained from adult tissues, it is in this area that obtaining and using oral MSCs have become a good source of experimentation. It is not unknown that MSCs have great regenerative power, high proliferative and differentiation capacity and high osteogenic potential. However, they can be affected by multiple external factors. The objective of this article is to describe the effect of tobacco on oral MSCs. Cigarettes and its components are a risk factor for various diseases at the level of the oral cavity, they have many adverse effects on the cellular biology of the mouth, therefore, it is not strange to think about the effect that these components can have on MSCs. of oral origin. The latest studies have shown that nicotine and condensed cigarette smoke have negative biological effects on MSCs, particularly oral ones. Despite the existing literature, it is not entirely clear what are the mechanisms involved in the negative effects of tobacco on oral MSCs and how they could be reversed.

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Effect of smoking on the proliferation capacity and osteogenic potential of human dental pulp stem cells (DPSCs)

Cited by 8 publications

References 0 publications

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Effect of diabetes on stem cells of the oral cavity. A brief review

Effect of tobacco on oral cavity stem cells. A brief review

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