Effect of sodium succinate on histomorphological changes of autodermic grafts in rats

Galenko-Yaroshevsky, P. A.; Mogilnaya, G. M.; Karas, A.F.; Vas'kov, K. S.; Gumenyuk, S. E.; Popkov, V. L.

doi:10.1007/bf02447255

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“…The use of bacteriophages against pathogenic microorganisms attracts the attention of surgeons around the world. Along with that, experts in free skin grafting suggest that the infection control should be combined with support of redox reactions and angiogenesis in the skin around the wound in order to improve the engraftment of an autodermotransplant [ 4 ]. It was proposed that succinic acid [ 5 , 6 ], and lidocaine (an anesthetic) [ 7 ] could support the activity of redox reactions and angiogenesis when used as components of hydrogel wound dressings.…”

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confidence: 99%

Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro

Бесчастнов¹,

Ryabkov²,

Leontiev³

et al. 2021

Sovrem Tehnol Med

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Using bacteriophages to overcome the increasing resistance of microorganisms to antibiotics is a novel research venue of clinical importance. Among other challenges, this technique is expected to create and maintain an adequate local concentration of bacteriophages at the site of application. In addition, the possibility of combining the phage preparation with antioxidants and anesthetics may provide new options for stimulating the reparative process.The aim of the study was to assess the viability and lytic activity of bacteriophages incorporated into a hydrogel-based wound dressing that contains polyvinyl alcohol, phosphate buffer, with optional additions of succinic acid and lidocaine. Materials and Methods.A technique for incorporating bacteriophages into the complex hydrogel wound dressing ex tempore has been proposed. The bacteriolytic activity of phages inside the hydrogel was determined using standard microbiological techniques. Specifically, we used nutrient media with lawn cultures of Staphylococcus aureus added with the following antibacterial combinations: bacteriophages + succinic acid, bacteriophages + lidocaine, and bacteriophages + succinic acid + lidocaine. The lytic activity of bacteriophages was assessed within 1 to 7 days after the formation of the hydrogel.Results. In all samples containing bacteriophages, the presence of viable and lytically active phages was noted within 1 to 7 days, as evidenced by the "negative colonies" on the culture lawns. On days 1 to 3, no secondary growth was recorded in the phage-containing samples. In hydrogel samples containing phages, succinic acid, and lidocaine, secondary bacterial colonies were detected starting from day 4 indicating some reduction in the lytic activity. Conclusion.The results suggest that bacteriophages immobilized in the hydrogel maintain their viability and lytic activity, and this activity persists when the phages are combined with succinic acid and lidocaine.

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