Effect of some chemical and physical elicitors on some secondary compound induction of Ricinus communis through callus induction

Abstract

The results of the present study showed that AgNO 3 and ABA has a negative effect on fresh and dry weight, where both of weight significantly decreased when treated with ABA and AgNO 3 compare to control treatment, except callus treated with AgNO 3 at 4mg/l, the rate of fresh and dry weight significantly increased reached to 434,77mg respectively compared to control. Physical treatment also had a significant effect on fresh and dry weight for callus, the highest fresh and dry weight significantly reached to 491,93 mg respectively when callus exposure for 10 minutes to UV ray. The exposure to light for different period had negative effect on the rates of fresh and dry weight, as the control superior significantly for all lighting treatment. The concentration of all the secondary compounds extracted from callus increased significantly compared to their concentration in the seed extract. The addition of chemical treatments to callus lead to the difference in the concentration of secondary compounds. The quercetin compound reached its highest and significantly to 95.17μg/ml at the concentration of 4mg/l of AgNO 3. while kaempferol suffer significantly decrease at all concentration of AgNO 3 , and reached to highest value significantly at control 114.76μg/ml, added concentration at 6 mg/l of AgNO 3 had a positive effect in increasing the concentration of Ricinin significantly to 119.90μg/ml.

Biotic and Abiotic Elicitors Induce Biosynthesis and Accumulation of Some Withanolide Compounds in the Callus Culture of Withania somnifera L.

“…However, plant tissues respond differently to the applied photoperiod (Siddique & Islam, 2018). According to Twaij & Taha (2017), sequential light periods led to an enhanced fresh and dry weight of callus tissue compared to constant light or dark conditions in Ricinus communis culture. An increase in different phytochemicals was recorded in various cultures of plants (Victório et al, 2015).…”

“…Conclusively, many previous studies have demonstrated the efficiency of using light as a physical abiotic factor in stimulating the biosynthesis of primary and secondary plant compounds through increased activity of the phenylalanine ammonia-lyase (PAL) enzyme (Abbasi et al, 2007), enhancement of chlorophyll biosynthesis and regulation of gene expression responsible for the synthesis of terpenes (Kawoosa et al, 2010), improvement of the accumulation of phenolic and flavonoids contents (Adil et al, 2019a) and formation of anthocyanins and carotene pigments (Li & Kubota, 2009). According to Twaij & Taha (2017), the incubation of callus culture of Ricinus communis under continuous light conditions was more effective for the stimulation of quercetin, kaempferol and ricinin compounds. In Eclipta alba callus culture, the long light periods also induce the biosynthesis of several secondary metabolites (Khurshid et al, 2020).…”

“…In this study, we monitored the effect of various monochromatic lights and plant growth regulators (PGRs. Furthermore, after exposing the callus tissue to light for 24h, the content of kaempferol, qurecetin and ricinin in Ricinus communis culture were significantly increased (Twaij & Taha, 2017).…”

“…Moreover, Kalidhasan et al (2013) showed that cultivated Withania plant increased anthocyanin content by 42% after 30 d of exposure to UV rays. In addition, UV rays increased the fresh and dry weights of Solanum nigrum and Hypericum triquetrifolium callus cultures, also, the production of several active chemicals was significantly stimulated (Twaij, 2011). Moreover, exposing the callus cultures to UV rays for 20 min considerably raised the content of salanin in Melia azedarach culture (Ahmed, 2014) and citrullol and cucurbitacin B in the Citrullus colocynthis culture (Mutasher, 2015).…”

Photoperiod and UV light influence secondary metabolites of Hyoscyamus niger callus induced from leaves

“…Earlier research findings have observed variations in the impact of light on the promotion of callus growth. Specifically, in the plants Citrullus colocynthisin (17) and Ricinus communis (18), it was found that regular light cycles consisting of 16 hours of light followed by 8 hours of darkness resulted in a greater increase in the mass of callus tissue (both fresh and dry weight) compared to continuous exposure to either light or darkness.…”