Effect of Sorbitol on Grapevine's Growth in Vitro

Dorochenko, Natalia Petrovna; Puzirnova, Valentina

doi:10.30679/2219-5335-2020-4-64-190-209

Cited by 4 publications

(1 citation statement)

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“…It is connected, first of all with complexity of similar experiments as at change of concentration of macrosalts in substrate it is necessary to consider features of their absorption by plants, antagonism and their synergism in various ratios and concentration. At the same time most often most of researchers at microcloning of grapes use as basic mineral basis copy-book of the Murasige environment and Skuga (MS) [1][2][3][4][5][6][7][8][9][10]. Less often works in which authors use insignificant modifications of mineral basis of this environment [11] meet, or study against the background of MS efficiency of other standard environments [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Improvement of the copy-book of nutrient medium for input of meristems of grapes in the culture of in vitro

Rebrov

2020

E3S Web Conf.

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Results of researches on development of substrate for input of meristems of grapes are provided to the culture of in vitro for the purpose of increase in their regenerative ability at improvement from chronic diseases. In the advanced environment in comparison with analogs the general content of macrosalts was reduced and their ratio taking into account consumption and features of their absorption from nutrient solution is optimized by grape plant. During tests of new substrate its efficiency for grapes grades from various environmental-geographical groups was established. Higher and stable percent of survival and regeneration of extremely small meristems, against the background of various modifications of copy-book of traditionally applied substrate of Murasige and Skuga is established.

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Section: Introductionmentioning

confidence: 99%

Improvement of the copy-book of nutrient medium for input of meristems of grapes in the culture of in vitro

Rebrov

2020

E3S Web Conf.

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Biotechnological methods of preservation of the grape gene pool in the in vitro collection

Doroshenko

Puzirnova

2020

BIO Web Conf.

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The article shows the results of the improvement of meristem survival, its growth characteristics, shoot formation with the combined usage of apical meristems and the preparations such as Ribavirin, salicylic acid, Melafen, the antibiotic Cefotaxime. The research results demonstrate a decrease in the intensity of growth with the presence of sucrose, fructose, and sorbitol in the culture medium. The clarification of the parameters of the preparations application made it possible to increase the duration of nonstop storage of plants up to 8–10 months.

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Influence of concentration and ratio of macro elements in culture medium on the regeneration of grape meristemsin vitro

Rebrov

2020

BIO Web Conf.

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The results of studies on the development of culture medium for initiation grape meristems into in vitro culture in order to increase their regenerative capacity during recovery from chronic diseases are presented. In the improved medium, in comparison with analogs, the total content of macro salts was reduced and their ratio was optimized, taking into account their consumption and the characteristics of their absorption from the nutrient solution by the grape plant. In the course of testing the new culture medium, its effectiveness has been established for grape varieties from various ecological and geographical groups. A higher and more stable percentage of survival and regeneration of extremely small meristems have been established against the background of various modifications of the recipe for the traditionally used culture medium of Murashige and Skoog.

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Effect of Sorbitol on Grapevine's Growth in Vitro

Cited by 4 publications

References 0 publications

Improvement of the copy-book of nutrient medium for input of meristems of grapes in the culture of in vitro

Improvement of the copy-book of nutrient medium for input of meristems of grapes in the culture of in vitro

Biotechnological methods of preservation of the grape gene pool in the in vitro collection

Influence of concentration and ratio of macro elements in culture medium on the regeneration of grape meristemsin vitro

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