Effect of starch-based biomaterials on the in vitro proliferation and viability of osteoblast-like cells

Marques, A. P.; Cardoso‐Cruz, Helder; Coutinho, O. P.; Reis, Rui L.

doi:10.1007/s10856-005-3580-7

Cited by 39 publications

(40 citation statements)

References 46 publications

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“…These thermoplastic polyesters have reasonable biocompatibility, biodegradability, mechanical strength and easy processability. Blends of PLLA and starch (SPLA) have also been proposed in our group to be used in the biomedical field, and were found from cell adhesion and proliferation tests, to interact positively with cells [7].…”

Section: Introductionmentioning

confidence: 99%

Dynamic mechanical behavior of starch-based scaffolds in dry and physiologically simulated conditions: Effect of porosity and pore size

et al. 2008

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The three-dimensional scaffolds of a blend of starch and poly(L-lactic) acid, SPLA70, were produced using compression molding of polymer/salt mixture followed by leaching of salt. One series of scaffolds were prepared with varying polymer-to-salt ratio while keeping the salt size constant, and the other series of scaffolds were prepared with varying salt sizes while keeping the polymer-to-salt ratio constant. The X-ray microcomputed tomography and scanning electron microscopy assay were used to analyze the porous morphologies, porosity and distribution of porosity of the porous scaffolds. Salt-free and integrated SPLA70 scaffolds with porosities ranging from 74% to 82% and pore sizes of 125-250 to 500-1000 microm can be fabricated using the present fabrication technique. The water uptake of the SPLA70 scaffolds increases with increasing porosities and also with increasing pore size. In dry state, the storage modulus decreases with increasing porosity and also with increasing pore size. The normalized modulus values are related to normalized density of the scaffolds by a power-law function with an exponent between 2 and 3. For the immersed scaffolds under physiological conditions, the storage modulus was less dependent on porosity and pore size. However, the loss factor increased significantly compared with dry state measurements. The present study clearly shows that the mechanical performance of porous polymeric constructs in dry and in immersed state is completely different, and for comparison with biomechanical performance of tissues, the tests should ideally be performed in immersed state.

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Section: Introductionmentioning

confidence: 99%

Dynamic mechanical behavior of starch-based scaffolds in dry and physiologically simulated conditions: Effect of porosity and pore size

et al. 2008

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“…PLDLA (70:30) has already been tested with regards to cytotoxicity, and the MTT, agar and filter diffusion assays have shown good cytocompatibility for this copolymer 27 . In addition, the mortality of human osteoblasts in extracts containing PLLA degradation products is ~30%, indicating low cytotoxicity 28 . These results support the low cytotoxicity reported here.…”

Section: Discussionmentioning

confidence: 99%

Lithograph-moulded poly-L-co-D,L lactide porous membranes for osteoblastic culture

et al. 2013

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Pore size, shape, wall morphology, porosity, and interconnectivity are important characteristics of the scaffolds. Lithography is a manufacturing technique that allows the production of tridimensional scaffolds with a controllable and reproducible inner architecture. The aim of this study was to use lithography to create a poly-L-co-D,L lactide (PLDLA) scaffold with symmetrical pore size and distribution, and to evaluate its biocompatibility with osteoblasts in vitro. Lithographic moulds were used to produce porous PLDLA membranes by a casting procedure. Osteoblasts were removed from calvarial bones and seeded onto porous and smooth PLDLA membranes after which cell viability and adhesion assays, cytochemical analysis and scanning electron microscopy were used to characterize the cells. Cell viability and adhesion assays, cytochemical analysis, and scanning electron microscopy were carried out. Cell viability was similar on porous and smooth PLDLA membranes but higher than on a polystyrene substrate (positive control). Although osteoblasts adhered to the surface of all the materials tested, cell adhesion to lithographed PLDLA was greater than to smooth PLDLA membranes. In conclusion, osteoblasts interacted well with PLDLA membranes, as shown by the viability and adhesion assays and by the enhanced collagen production.

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“…19,20 The yellow stain of the reagent MTT is converted inside the mitochondria into a dark blue stain, formazan (translated by the largest absorption of MTT), which demonstrates that cellular mitochondrial activity is present. Although toxic substances do not act in a specific cellular level, they can affect their functions, 21 thus early determination of mitochondrial activity in terms of cellular sensitivity, in our opinion, was enough to evaluate cellular viability with respect to subsequent proliferation. Absence of cytotoxicity does not check on the material biocompatibility; yet the determination of cytotoxic potential is an important issue in standard tests such as ISO.…”

Section: Cytotoxicity Analyses Of Blendsmentioning

confidence: 90%

Cell culture on PCL/PLGA blends

Lucchesi

Barbanti

Joazeiro

et al. 2009

J of Applied Polymer Sci

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Bioresorbable polymers have been studied as support for cell culture in the tissue engineering area. Osteoblastic cells were cultivated on poly(e-caprolactone), poly(lactic acid-co-glycolic acid), and (70/30), (50/50), and (30/70) blends. Cytotoxicity and cell adhesion assays and scanning electronic microscopy studies were described. The cells presented significant growth on the blends, showing no cytotoxic response. Results indicated that these blends are promising as devices for bone tissue applications. V

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Effect of starch-based biomaterials on the in vitro proliferation and viability of osteoblast-like cells

Cited by 39 publications

References 46 publications

Dynamic mechanical behavior of starch-based scaffolds in dry and physiologically simulated conditions: Effect of porosity and pore size

Dynamic mechanical behavior of starch-based scaffolds in dry and physiologically simulated conditions: Effect of porosity and pore size

Lithograph-moulded poly-L-co-D,L lactide porous membranes for osteoblastic culture

Cell culture on PCL/PLGA blends

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