Effect of Steam Explosion Pretreatment Catalysed by Organic Acid and Alkali on Chemical and Structural Properties and Enzymatic Hydrolysis of Sugarcane Bagasse

Silva, Thiago Alves Lopes; Zamora, Hernán Darí­o Zamora; Varão, Leandro Henrique Ribeiro; Prado, Natália Soares; Baffi, Milla Alves; Pasquini, Daniel

doi:10.1007/s12649-017-9989-7

Cited by 61 publications

(24 citation statements)

References 57 publications

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“…This band may be associated with the hydroxyl groups of cellulose, hemicellulose and lignin. These findings coincide with previously reported results for sugarcane bagasse submitted to a pretreatment process with steam explosion and sodium hydroxide [18][19][20].…”

Section: Biological Pretreatmentsupporting

confidence: 93%

Biological Pretreatment of Mexican Caribbean Macroalgae Consortiums Using Bm-2 Strain (Trametes hirsuta) and Its Enzymatic Broth to Improve Biomethane Potential

et al. 2018

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The macroalgae consortium biomass in the Mexican Caribbean represents an emerging and promising biofuel feedstock. Its biological pretreatment and potential for energetic conversion to biomethane were investigated, since some macroalgae have hard cell walls that present an obstacle to efficient methane production when those substrates are used. It has been revealed by anaerobic digestion assays that pretreatment with a Bm-2 strain (Trametes hirsuta) isolated from decaying wood in Yucatan, Mexico was 104 L CH 4 •kg VS −1 ; In fact, the fungal pretreatment produced a 20% increase in methane yield, with important amounts of alkali metals Ca, K, Mg, Na of 78 g/L, ash 35.5% and lignin 15.6%. It is unlikely that high concentrations of ash and alkali metals will produce an ideal feedstock for combustion or pyrolysis, but they can be recommended for a biological process.

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Section: Biological Pretreatmentsupporting

confidence: 93%

Biological Pretreatment of Mexican Caribbean Macroalgae Consortiums Using Bm-2 Strain (Trametes hirsuta) and Its Enzymatic Broth to Improve Biomethane Potential

et al. 2018

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“…A decrease at 1600 cm −1 was also observed. Different groups can be associated with this band, e.g., amides, carboxyl groups, C=C aromatics, lignin, and amino acids [64,65].…”

Section: Physical-chemical Characterizationmentioning

confidence: 99%

Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2

et al. 2019

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Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of Trametes hirsuta (Bm-2) was evaluated for bioethanol production from Brosimum alicastrum in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of T. hirsuta Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding.Microorganisms 2019, 7, 483 2 of 16 use bioethanol as their main source of fuel. Ethanol blends vary depending on the country, containing from as low as 5% (E5) to 100% bioethanol (E100) [4][5][6][7].The growing global demand for bioethanol requires the use of alternative sources of raw materials to complement sugar cane and cornstarch, which are the main raw materials used to produce it. Starch crops are widely used for bioethanol production because of their worldwide availability, easy conversion, and high ethanol yield. These raw materials include cereals (60%-80% starch), tubers and roots (60%-90%), legumes (25%-50%), and green and immature fruits (up to 70% starch) [2].Conventionally, ethanol production from starch consists of several stages. Starch is subjected to a gelatinization process followed by a liquefaction step where starch is converted to dextrins and smaller molecules by the action of bacterial thermostable amylases at high temperatures (95-105 • C) and pH values between 6 and 6.5. This step is followed by saccharification. The liquefied starch is cooled, pH is adjusted to 4-4.5, temperatures to 60-65 • C, and a fungal glucoamylase is added to hydrolyze the oligosaccharides to glucose. The liquefaction and saccharification stages represent about 40%-50% of the total energy used during starch-based ethanol production [8][9][10].Owing to this technical complexity and the economic implications of this approach, other biological alternatives have been investigated, such as simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP). The latter is a promising strategy for effective ethanol production, since it employs only one type of microorganism that is capable of both producing the enzymes to hydrolyze the biomass and converting sugars into ethanol [11,12]. This strategy has the potential of lowering the cost and enhancing the efficie...

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“…13 Thus, the acids loading, 90 mM and 120 mM, were applied according to the studies, in which different dilute organic acids were used for pretreatments. [17][18][19] Prior to the hydrolysis, 22.9 mg protein per g biomass of Celluclast 1.5 L (169.6 mg protein per mL), 18.3 mg protein per g biomass of Novozyme 188 (187.9 mg protein per mL) were added to the slurry for enzymatic hydrolysis. Aer the hydrolysis, the samples were boiled for 10 min to stop the enzymatic hydrolysis and then centrifuged for 10 min at 10 000g.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

“…14,30,31 Different detoxication methods, including lime, evaporation, peroxidase, adsorption using ion exchange resins, activated charcoal, and biological, have been used previously to reduce the process inhibitors associated with pretreatment and neutralization. 19 However, process conditioning, e.g., selecting suitable pretreatment and neutralizing reagents and their proper concentrations, is a relatively simple method to avoid inhibition with salts in successful biofuel production from lignocellulosic materials. A variety of acids have been investigated for pretreatment, such as sulfuric, oxalic, citric, acetic, and tartaric acids.…”

Section: Process Conditioningmentioning

confidence: 99%

“…A variety of acids have been investigated for pretreatment, such as sulfuric, oxalic, citric, acetic, and tartaric acids. [17][18][19] The pretreated solids are usually separated from the liquid for the following hydrolysis using the buffer solutions. However, the separation and addition of buffer solution would increase the processing cost.…”

Section: Process Conditioningmentioning

confidence: 99%

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Effect of salts formed by neutralization for the enzymatic hydrolysis of cellulose and acetone–butanol–ethanol fermentation

et al. 2019

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Effect of Steam Explosion Pretreatment Catalysed by Organic Acid and Alkali on Chemical and Structural Properties and Enzymatic Hydrolysis of Sugarcane Bagasse

Cited by 61 publications

References 57 publications

Biological Pretreatment of Mexican Caribbean Macroalgae Consortiums Using Bm-2 Strain (Trametes hirsuta) and Its Enzymatic Broth to Improve Biomethane Potential

Biological Pretreatment of Mexican Caribbean Macroalgae Consortiums Using Bm-2 Strain (Trametes hirsuta) and Its Enzymatic Broth to Improve Biomethane Potential

Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2

Effect of salts formed by neutralization for the enzymatic hydrolysis of cellulose and acetone–butanol–ethanol fermentation

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